################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 114 GTAC GT^AC AflIII 734 ACGTGT A^C[GA][CT]GT Alw44I 166 GTGCAC G^TGCAC AlwNI 681 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 166 GTGCAC G^TGCAC AspHI 166 GTGCAC G[AT]GC[AT]^C BalI 713 TGGCCA TGG^CCA BbsI 15 GTCTTC GAAGAC(2/6) BbvCI 222 GCTGAGG CCTCAGC(-5/-2) BglI 34 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 15 GTCTTC GAAGAC(2/6) BsaAI 733 TACGTG [CT]AC^GT[GA] BsaI 262 GGTCTC GGTCTC(1/5) BsiHKAI 166 GTGCAC G[AT]GC[AT]^C BsmAI 263 GTCTC GTCTC(1/5) BsrI 398 CCAGT ACTGG(1/-1) BstAPI 566 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 753 GGTCACC G^GT[ACGT]ACC BstPI 753 GGTCACC G^GT[ACGT]ACC BstXI 716 CCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 223 CTGAG C^T[ACGT]AG DsaI 710 CCGTGG C^C[GA][CT]GG EcoNI 740 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 753 GGTCACC G^GT[ACGT]ACC EcoT14I 590 CCAAGG C^C[AT][AT]GG MaeII 734 ACGT A^CGT MluNI 713 TGGCCA TGG^CCA MscI 713 TGGCCA TGG^CCA NaeI 442 GCCGGC GCC^GGC NgoMIV 442 GCCGGC G^CCGGC NspI 521 GCATGT [GA]CATG^[CT] PpuMI 67 GGGACCC [GA]G^G[AT]CC[CT] PstI 127 CTGCAG CTGCA^G PvuI 763 CGATCG CGAT^CG RsaI 114 GTAC GT^AC SfcI 127 CTGCAG C^T[GA][CT]AG SfiI 33 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 479 CCCGGG CCC^GGG StuI 5 AGGCCT AGG^CCT StyI 590 CCAAGG C^C[AT][AT]GG XcmI 377 CCATCCGGCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 479 CCCGGG C^CCGGG XmaI 479 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 107 CGCG CG^CG 336 CGCG AcyI 470 GGCGCC G[GA]^CG[CT]C 726 GGCGCC AlwI 96 GATCC GGATC(4/5) 743 GGATC ApaI 41 GGGCCC GGGCC^C 625 GGGCCC AvaI 479 CCCGGG C^[CT]CG[GA]G 618 CTCGGG BanI 470 GGCGCC G^G[CT][GA]CC 726 GGCGCC BbeI 470 GGCGCC GGCGC^C 726 GGCGCC BpmI 678 CTCCAG CTGGAG(16/14) 322 CTGGAG BsaHI 470 GGCGCC G[GA]^CG[CT]C 726 GGCGCC BsgI 687 CTGCAC GTGCAG(16/14) 432 GTGCAG BsoBI 479 CCCGGG C^[CT]CG[GA]G 618 CTCGGG BsrFI 442 GCCGGC [GA]^CCGG[CT] 757 ACCGGC BstUI 107 CGCG CG^CG 336 CGCG Cfr10I 442 GCCGGC [GA]^CCGG[CT] 757 ACCGGC Hin1I 470 GGCGCC G[GA]^CG[CT]C 726 GGCGCC KasI 470 GGCGCC G^GCGCC 726 GGCGCC MboII 16 TCTTC GAAGA(8/7) 698 TCTTC MslI 516 CACCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 717 CACCGCTGTG MvnI 107 CGCG CG^CG 336 CGCG NarI 470 GGCGCC GG^CGCC 726 GGCGCC PvuII 220 CAGCTG CAG^CTG 346 CAGCTG SfoI 470 GGCGCC GGC^GCC 726 GGCGCC Tsp45I 614 GTGAC ^GT[GC]AC 754 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiEI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI Sau3AI Sau96I ScrFI TseI TspRI Van91I