(Circular) (Six-base) MAPSORT of: pFN21AE1752.seq Check: 5529 from: 1 to: 5476 pFN21AE1752 5476 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2662 3539 4923 5191 1632 Size: 701 329 877 1384 268 1917 Fragments arranged by size: 1917 1384 877 701 329 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 2739 4375 5352 1203 Size: 1536 1636 977 1327 Fragments arranged by size: 1636 1536 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 2726 1166 Size: 1560 3916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 2691 3219 4791 2691 Size: 528 1572 3376 Fragments arranged by size: 3376 1572 528 ApaI G_GGCC'C Cuts at: 2050 2634 2050 Size: 584 4892 ApaLI G'TGCA_C Cuts at: 2171 3533 4689 2171 Size: 1362 1156 2958 Fragments arranged by size: 2958 1362 1156 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2484 2623 2801 1870 Size: 73 541 139 178 4545 Fragments arranged by size: 4545 541 178 139 73 BamHI G'GATC_C Cuts at: 2806 3173 2806 Size: 367 5109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2475 2731 2797 4252 618 Size: 325 217 627 688 256 66 1455 1842 Fragments arranged by size: 1842 1455 688 627 325 256 217 66 BanII G_rGCy'C Cuts at: 1391 2050 2247 2634 2795 1391 Size: 659 197 387 161 4072 Fragments arranged by size: 4072 659 387 197 161 BbeI G_GCGC'C Cuts at: 2479 2735 2479 Size: 256 5220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2013 928 Size: 971 114 4391 Fragments arranged by size: 4391 971 114 BbvCI CC'TCA_GC Cuts at: 2228 2228 Size: 5476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2045 4087 Size: 107 122 71 631 605 337 36 2042 Cuts at: 4087 136 Size: 1525 Fragments arranged by size: 2042 1525 631 605 337 122 107 71 36 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 2050 2175 2634 3537 4693 1790 Size: 260 125 459 903 1156 2573 Fragments arranged by size: 2573 1156 903 459 260 125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2397 3207 4165 Size: 416 53 83 399 24 980 810 958 Cuts at: 4165 442 Size: 1753 Fragments arranged by size: 1753 980 958 810 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2668 4136 1239 Size: 1109 320 1468 2579 Fragments arranged by size: 2579 1468 1109 320 Bpu10I CC'TnA_GC Cuts at: 2228 2228 Size: 5476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 4139 882 Size: 617 774 1866 2219 Fragments arranged by size: 2219 1866 774 617 BsaAI yAC'GTr Cuts at: 493 1204 2740 3267 493 Size: 711 1536 527 2702 Fragments arranged by size: 2702 1536 711 527 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2476 2732 3283 3665 Size: 53 83 186 936 943 256 551 382 Cuts at: 3665 4300 275 Size: 635 1451 Fragments arranged by size: 1451 943 936 635 551 382 256 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5251 Fragments arranged by size: 5251 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5476 BseYI C'CCAG_C Cuts at: 2211 2223 2239 2372 2451 2567 4679 5175 2211 Size: 12 16 133 79 116 2112 496 2512 Fragments arranged by size: 2512 2112 496 133 116 79 16 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2458 2677 1163 Size: 573 722 219 3962 Fragments arranged by size: 3962 722 573 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2390 2589 2767 2771 2775 2926 Size: 275 186 389 199 178 4 4 151 Cuts at: 2926 3687 4715 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 389 275 199 186 178 151 4 4 BsiHKAI G_wGCw'C Cuts at: 2175 3537 3622 4693 2175 Size: 1362 85 1071 2958 Fragments arranged by size: 2958 1362 1071 85 BsmI GAATG_Cn' Cuts at: 2987 3080 2987 Size: 93 5383 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2175 2247 2634 2795 3537 3622 Size: 399 260 125 72 387 161 742 85 Cuts at: 3622 4693 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 260 161 125 85 72 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 844 Size: 1987 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3363 1394 Size: 429 1540 3507 Fragments arranged by size: 3507 1540 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3971 4145 65 Size: 1183 2723 174 1396 Fragments arranged by size: 2723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2447 2762 4120 4307 1700 Size: 110 637 315 1358 187 2869 Fragments arranged by size: 2869 1358 637 315 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 2577 794 Size: 1783 3693 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 2758 1184 Size: 1574 3902 BstXI CCAn_nnnn'nTGG Cuts at: 2728 2728 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471 Size: 834 367 385 17 1452 86 12 346 Cuts at: 5471 1972 Size: 1977 Fragments arranged by size: 1977 1452 834 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2715 513 Size: 552 939 711 3274 Fragments arranged by size: 3274 939 711 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2024 2129 2200 2992 3786 3806 2024 Size: 105 71 792 794 20 3694 Fragments arranged by size: 3694 794 792 105 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2387 2445 2586 Size: 54 1425 50 275 18 557 58 141 Cuts at: 2586 2661 2718 2764 2772 2923 3812 5441 8 Size: 75 57 46 8 151 889 1629 43 Fragments arranged by size: 1629 1425 889 557 275 151 141 75 58 57 54 50 46 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2387 2586 2764 2772 2923 1537 Size: 275 575 199 178 8 151 4090 Fragments arranged by size: 4090 575 275 199 178 151 8 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 1353 Size: 2053 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2348 2662 2668 3539 4136 4923 Size: 393 701 15 314 6 871 597 787 Cuts at: 4923 5191 1239 Size: 268 1524 Fragments arranged by size: 1524 871 787 701 597 393 314 268 15 6 EcoNI CCTnn'n_nnAGG Cuts at: 2749 2749 Size: 5476 EcoO109I rG'GnC_Cy Cuts at: 2046 2047 2073 2631 2046 Size: 1 26 558 4891 Fragments arranged by size: 4891 558 26 1 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2413 2479 2735 4623 1678 Size: 735 66 256 1888 2531 Fragments arranged by size: 2531 1888 735 256 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4117 4149 4191 4223 1373 Size: 32 135 32 2545 32 42 32 2626 Fragments arranged by size: 2626 2545 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KasI G'GCGC_C Cuts at: 2475 2731 2475 Size: 256 5220 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2720 10 Size: 54 1425 343 888 2766 Fragments arranged by size: 2766 1425 888 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 2726 3434 3793 3952 518 Size: 648 1359 201 708 359 159 2042 Fragments arranged by size: 2042 1359 708 648 359 201 159 MspA1I CmG'CkG Cuts at: 1606 2215 2227 2353 2726 2892 3569 4717 4962 Size: 609 12 126 373 166 677 1148 245 Cuts at: 4962 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 609 373 245 166 126 12 NaeI GCC'GGC Cuts at: 2449 2449 Size: 5476 NarI GG'CG_CC Cuts at: 2476 2732 2476 Size: 256 5220 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3985 Fragments arranged by size: 3985 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NgoMIV G'CCGG_C Cuts at: 2447 2447 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2530 2834 4379 1132 Size: 97 1301 304 1545 2229 Fragments arranged by size: 2229 1545 1301 304 97 PasI CC'CwG_GG Cuts at: 1348 2041 1348 Size: 693 4783 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 2218 2254 2419 2515 1108 Size: 1098 12 36 165 96 4069 Fragments arranged by size: 4069 1098 165 96 36 12 PfoI T'CCnGG_A Cuts at: 2744 2744 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2073 2073 Size: 5476 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2046 2630 2046 Size: 584 4892 PstI C_TGCA'G Cuts at: 830 2136 2828 830 Size: 1306 692 3478 Fragments arranged by size: 3478 1306 692 PvuI CG_AT'CG Cuts at: 2001 2771 2001 Size: 770 4706 PvuII CAG'CTG Cuts at: 2227 2353 2227 Size: 126 5350 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SanDI GG'GwC_CC Cuts at: 2073 2073 Size: 5476 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2132 2824 3959 4640 4831 826 Size: 219 1087 692 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 692 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2045 2045 Size: 5476 SfoI GGC'GCC Cuts at: 2477 2733 2477 Size: 256 5220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2486 2803 1872 Size: 614 317 4545 Fragments arranged by size: 4545 614 317 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 2012 1517 Size: 495 4981 StyI C'CwwG_G Cuts at: 513 1065 2004 2595 2915 513 Size: 552 939 591 320 3074 Fragments arranged by size: 3074 939 591 552 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 2190 3503 3520 3673 3858 4303 Size: 1255 593 17 1313 17 153 185 445 Cuts at: 4303 325 Size: 1498 Fragments arranged by size: 1498 1313 1255 593 445 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3722 5434 96 Size: 274 80 33 51 1443 1745 1712 138 Fragments arranged by size: 1745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5436 5468 5436 Size: 32 5444 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2484 2801 1870 Size: 614 317 4545 Fragments arranged by size: 4545 614 317 XmnI GAAnn'nnTTC Cuts at: 3605 3605 Size: 5476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SrfI SwaI