################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI Eco47III Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MamI MboI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 131 GGTACC G^GTACC AccI 283 GTATAC GT^[AC][GT]AC AccII 640 CGCG CG^CG AcyI 362 GGCGTC G[GA]^CG[CT]C AhdI 587 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 328 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 131 GGTACC G^GTACC AspEI 587 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 495 CTCGGG C^[CT]CG[GA]G AvaII 299 GGACC G^G[AT]CC BalI 344 TGGCCA TGG^CCA BanI 131 GGTACC G^G[CT][GA]CC BanII 163 GGGCTC G[GA]GC[CT]^C BbsI 16 GTCTTC GAAGAC(2/6) BciVI 844 GGATAC GTATCC(6/5) BfaI 382 CTAG C^TAG BpuAI 16 GTCTTC GAAGAC(2/6) BsaHI 362 GGCGTC G[GA]^CG[CT]C BsaWI 128 TCCGGT [AT]^CCGG[AT] BsiEI 209 CGGCCG CG[GA][CT]^CG BsoBI 495 CTCGGG C^[CT]CG[GA]G Bsp1407I 230 TGTACA T^GTACA BsrFI 239 ACCGGC [GA]^CCGG[CT] BsrGI 230 TGTACA T^GTACA Bst1107I 283 GTATAC GTA^TAC BstEII 177 GGTCACC G^GT[ACGT]ACC BstPI 177 GGTCACC G^GT[ACGT]ACC BstUI 640 CGCG CG^CG BstZ17I 283 GTATAC GTA^TAC Cac8I 549 GCAGGC GC[ACGT]^[ACGT]GC CfoI 538 GCGC GCG^C Cfr10I 239 ACCGGC [GA]^CCGG[CT] DraII 298 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 159 CCACGG C^C[GA][CT]GG EagI 209 CGGCCG C^GGCCG Eam1105I 587 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 504 CTCTTC CTCTTC(1/4) EclXI 209 CGGCCG C^GGCCG Eco52I 209 CGGCCG C^GGCCG EcoO109I 298 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 177 GGTCACC G^GT[ACGT]ACC HaeII 537 GGCGCT [GA]GCGC^[CT] HhaI 538 GCGC GCG^C Hin1I 362 GGCGTC G[GA]^CG[CT]C HinP1I 538 GCGC G^CGC KpnI 131 GGTACC GGTAC^C Ksp632I 504 CTCTTC CTCTTC(1/4) MaeI 382 CTAG C^TAG MluNI 344 TGGCCA TGG^CCA MscI 344 TGGCCA TGG^CCA MslI 636 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 640 CGCG CG^CG PpuMI 298 GGGACCT [GA]G^G[AT]CC[CT] PstI 688 CTGCAG CTGCA^G PvuII 433 CAGCTG CAG^CTG SbfI 687 CCTGCAGG CCTGCA^GG SexAI 224 ACCTGGT A^CC[AT]GGT SfcI 688 CTGCAG C^T[GA][CT]AG Sse8387I 687 CCTGCAGG CCTGCA^GG SspBI 230 TGTACA T^GTACA TaqI 29 TCGA T^CGA TthHB8I 29 TCGA T^CGA XcmI 831 CCACAACCACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 434 AGCT AG^CT 779 AGCT BcnI 212 CCGGG CC^[GC]GG 408 CCGGG BsaAI 271 TACGTG [CT]AC^GT[GA] 625 TACGTG BsmAI 205 GTCTC GTCTC(1/5) 330 GTCTC BsmFI 298 GGGAC GGGAC(10/14) 498 GGGAC Cfr13I 299 GGACC G^G[ACGT]CC 371 GGCCC DdeI 367 CTGAG C^T[ACGT]AG 729 CTCAG EcoNI 684 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 816 CCTGGGCGAGG HgaI 75 GACGC GACGC(5/10) 363 GCGTC HincII 412 GTCAAC GT[CT]^[GA]AC 810 GTTGAC HindII 412 GTCAAC GT[CT]^[GA]AC 810 GTTGAC HinfI 121 GACTC G^A[ACGT]TC 557 GACTC MspA1I 4 CAGCGG C[AC]G^C[GT]G 433 CAGCTG NciI 212 CCGGG CC^[GC]GG 408 CCGGG PleI 121 GACTC GAGTC(4/5) 557 GACTC Sau96I 299 GGACC G^G[ACGT]CC 371 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI BbvI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NlaIII NlaIV RsaI ScrFI TseI Tsp45I TspRI