(Circular) (Six-base) MAPSORT of: pFN21AE5347.seq Check: 3663 from: 1 to: 5569 pFN21AE5347 5569 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2289 2912 1937 Size: 352 623 4594 Fragments arranged by size: 4594 623 352 Acc65I G'GTAC_C Cuts at: 2136 2890 2136 Size: 754 4815 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2792 3632 5016 5284 1632 Size: 1160 840 1384 268 1917 Fragments arranged by size: 1917 1384 1160 840 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 2597 4298 2597 Size: 1701 3868 AleI CACnn'nnGTG Cuts at: 1166 2645 1166 Size: 1479 4090 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AlwNI CAG_nnn'CTG Cuts at: 2338 3312 4884 2338 Size: 974 1572 3023 Fragments arranged by size: 3023 1572 974 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2878 3104 3256 5365 1493 Size: 1385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2500 2894 1870 Size: 73 557 394 4545 Fragments arranged by size: 4545 557 394 73 BamHI G'GATC_C Cuts at: 2899 3266 2899 Size: 367 5202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2136 2890 4345 618 Size: 325 217 627 349 754 1455 1842 Fragments arranged by size: 1842 1455 754 627 349 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2888 1391 Size: 781 716 4072 Fragments arranged by size: 4072 781 716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2014 928 Size: 971 115 4483 Fragments arranged by size: 4483 971 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3760 3794 1903 Size: 34 50 34 1739 34 3678 Fragments arranged by size: 3678 1739 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2843 3461 4671 2843 Size: 618 1210 3741 Fragments arranged by size: 3741 1210 618 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4180 136 Size: 107 122 71 631 605 2508 1525 Fragments arranged by size: 2508 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2986 2986 Size: 5569 Bme1580I G_kGCm'C Cuts at: 1790 2581 2704 3630 4786 1790 Size: 791 123 926 1156 2573 Fragments arranged by size: 2573 1156 926 791 123 BmgBI CAC'GTC Cuts at: 1318 2038 1318 Size: 720 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3300 4258 442 Size: 416 53 83 399 24 1883 958 1753 Fragments arranged by size: 1883 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2352 2352 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2428 2462 2689 4229 1239 Size: 1189 34 227 1540 2579 Fragments arranged by size: 2579 1540 1189 227 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3694 4559 4857 3694 Size: 865 298 4406 Fragments arranged by size: 4406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 2632 3360 493 Size: 711 1074 354 728 2702 Fragments arranged by size: 2702 1074 728 711 354 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2368 3376 3758 4393 Size: 53 83 186 936 835 1008 382 635 Cuts at: 4393 275 Size: 1451 Fragments arranged by size: 1451 1008 936 835 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 4002 4400 4674 4821 5232 2133 Size: 1869 398 274 147 411 2470 Fragments arranged by size: 2470 1869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 2843 2849 2873 Size: 30 165 30 1032 30 378 6 24 Cuts at: 2873 2879 1178 Size: 6 3868 Fragments arranged by size: 3868 1032 378 165 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2792 2795 2851 2854 1186 Size: 1606 3 56 3 3901 Fragments arranged by size: 3901 1606 56 3 3 BseYI C'CCAG_C Cuts at: 2177 2299 2798 4772 5268 2177 Size: 122 499 1974 496 2478 Fragments arranged by size: 2478 1974 499 496 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 2473 2573 1163 Size: 573 720 17 100 4159 Fragments arranged by size: 4159 720 573 100 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2217 3019 3780 4808 1540 Size: 275 186 216 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 275 216 186 BsiHKAI G_wGCw'C Cuts at: 3630 3715 4786 3630 Size: 85 1071 4413 Fragments arranged by size: 4413 1071 85 BsmI GAATG_Cn' Cuts at: 3080 3173 3080 Size: 93 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2581 2704 2888 3630 3715 4786 Size: 399 382 409 123 184 742 85 1071 Cuts at: 4786 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 409 399 382 184 123 85 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2924 844 Size: 2080 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3456 1394 Size: 429 1633 3507 Fragments arranged by size: 3507 1633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4064 4238 65 Size: 1183 2816 174 1396 Fragments arranged by size: 2816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2244 4213 4400 1700 Size: 110 434 1969 187 2869 Fragments arranged by size: 2869 1969 434 187 110 BsrGI T'GTAC_A Cuts at: 96 2235 96 Size: 2139 3430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 3629 4641 3629 Size: 1012 4557 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 2182 1184 Size: 998 4571 BstYI r'GATC_y Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564 Size: 927 367 385 17 1452 86 12 346 Cuts at: 5564 1972 Size: 1977 Fragments arranged by size: 1977 1452 927 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2290 2290 Size: 5569 BtgI C'CryG_G Cuts at: 513 1065 2164 513 Size: 552 1099 3918 Fragments arranged by size: 3918 1099 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2688 487 Size: 683 56 1462 3368 Fragments arranged by size: 3368 1462 683 56 BtsI GCAGTG_nn' Cuts at: 3085 3879 3899 3085 Size: 794 20 4755 Fragments arranged by size: 4755 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2214 2349 Size: 54 1425 50 275 18 363 21 135 Cuts at: 2349 3016 3905 5534 8 Size: 667 889 1629 43 Fragments arranged by size: 1629 1425 889 667 363 275 135 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2214 3016 1537 Size: 275 402 802 4090 Fragments arranged by size: 4090 802 402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2515 3499 1353 Size: 1162 984 3423 Fragments arranged by size: 3423 1162 984 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2428 2462 2689 2792 3632 4229 5016 Size: 393 796 34 227 103 840 597 787 Cuts at: 5016 5284 1239 Size: 268 1524 Fragments arranged by size: 1524 840 796 787 597 393 268 227 103 34 EcoNI CCTnn'n_nnAGG Cuts at: 2693 2825 2693 Size: 132 5437 EcoO109I rG'GnC_Cy Cuts at: 2304 2304 Size: 5569 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2546 4716 1678 Size: 868 2170 2531 Fragments arranged by size: 2531 2170 868 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2353 2385 2583 2615 4210 Size: 32 135 32 781 32 198 32 1595 Cuts at: 4210 4242 4284 4316 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1595 781 198 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 2817 2913 3159 669 Size: 988 281 481 398 96 246 3079 Fragments arranged by size: 3079 988 481 398 281 246 96 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KpnI G_GTAC'C Cuts at: 2140 2894 2140 Size: 754 4815 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2549 4683 4867 1305 Size: 1244 2134 184 2007 Fragments arranged by size: 2134 2007 1244 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 10 Size: 54 1425 343 519 3228 Fragments arranged by size: 3228 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 3527 3886 4045 518 Size: 648 1479 882 359 159 2042 Fragments arranged by size: 2042 1479 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2440 2985 3662 4810 5055 1606 Size: 405 429 545 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 429 405 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5017 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2927 4472 1132 Size: 97 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 97 PasI CC'CwG_GG Cuts at: 1348 2821 1348 Size: 1473 4096 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5569 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2304 2304 Size: 5569 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2081 2113 2081 Size: 32 5537 PstI C_TGCA'G Cuts at: 830 2697 2921 830 Size: 1867 224 3478 Fragments arranged by size: 3478 1867 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2440 2440 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2697 2921 2697 Size: 224 5345 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SexAI A'CCwGG_T Cuts at: 2229 2229 Size: 5569 SfcI C'TryA_G Cuts at: 826 1045 2693 2917 4052 4733 4924 826 Size: 219 1648 224 1135 681 191 1471 Fragments arranged by size: 1648 1471 1135 681 224 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3673 4574 4836 820 Size: 197 926 1730 901 262 1553 Fragments arranged by size: 1730 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 3008 513 Size: 552 1943 3074 Fragments arranged by size: 3074 1943 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 2416 3596 3613 3766 3951 4396 Size: 1255 597 239 1180 17 153 185 445 Cuts at: 4396 325 Size: 1498 Fragments arranged by size: 1498 1255 1180 597 445 239 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 2355 2523 Size: 274 80 33 51 1443 258 120 168 Cuts at: 2523 3815 5527 96 Size: 1292 1712 138 Fragments arranged by size: 1712 1443 1292 274 258 168 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 2849 2881 5529 5561 2435 Size: 32 382 32 2648 32 2443 Fragments arranged by size: 2648 2443 382 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2843 2843 Size: 5569 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI