(Linear) (Six-base) MAPSORT of: KIEE5347.seq Check: 3398 from: 1 to: 864 KIEE5347.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AccI GT'mk_AC Cuts at: 0 284 864 Size: 284 580 Acc65I G'GTAC_C Cuts at: 0 131 864 Size: 131 733 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 787 864 Size: 787 77 AhdI GACnn_n'nnGTC Cuts at: 0 592 864 Size: 592 272 AleI CACnn'nnGTG Cuts at: 0 640 864 Size: 640 224 AlwNI CAG_nnn'CTG Cuts at: 0 333 864 Size: 333 531 AvaI C'yCGr_G Cuts at: 0 495 864 Size: 495 369 BanI G'GyrC_C Cuts at: 0 131 864 Size: 131 733 BanII G_rGCy'C Cuts at: 0 167 864 Size: 167 697 BbsI GAAGACnn'nnnn_ Cuts at: 0 9 864 Size: 9 855 BciVI GTATCCnnnnn_n' Cuts at: 0 838 864 Size: 838 26 Bme1580I G_kGCm'C Cuts at: 0 576 699 864 Size: 576 123 165 Fragments arranged by size: 576 165 123 BmgBI CAC'GTC Cuts at: 0 33 864 Size: 33 831 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 347 864 Size: 347 517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 423 457 684 864 Size: 423 34 227 180 Fragments arranged by size: 423 227 180 34 BsaAI yAC'GTr Cuts at: 0 273 627 864 Size: 273 354 237 Fragments arranged by size: 354 273 237 BsaHI Gr'CG_yC Cuts at: 0 363 864 Size: 363 501 BsaWI w'CCGG_w Cuts at: 0 128 864 Size: 128 736 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 430 460 838 844 864 Size: 430 30 378 6 20 Fragments arranged by size: 430 378 30 20 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 787 790 846 849 864 Size: 787 3 56 3 15 Fragments arranged by size: 787 56 15 3 3 BseYI C'CCAG_C Cuts at: 0 172 294 793 864 Size: 172 122 499 71 Fragments arranged by size: 499 172 122 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 468 568 864 Size: 451 17 100 296 Fragments arranged by size: 451 296 100 17 BsiEI CG_ry'CG Cuts at: 0 212 864 Size: 212 652 Bsp1286I G_dGCh'C Cuts at: 0 167 576 699 864 Size: 167 409 123 165 Fragments arranged by size: 409 167 165 123 BsrFI r'CCGG_y Cuts at: 0 239 864 Size: 239 625 BsrGI T'GTAC_A Cuts at: 0 230 864 Size: 230 634 BstEII G'GTnAC_C Cuts at: 0 177 864 Size: 177 687 BstZ17I GTA'TAC Cuts at: 0 285 864 Size: 285 579 BtgI C'CryG_G Cuts at: 0 159 864 Size: 159 705 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 683 864 Size: 683 181 EaeI y'GGCC_r Cuts at: 0 188 209 344 864 Size: 188 21 135 520 Fragments arranged by size: 520 188 135 21 EagI C'GGCC_G Cuts at: 0 209 864 Size: 209 655 EarI CTCTTCn'nnn_ Cuts at: 0 510 864 Size: 510 354 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 423 457 684 787 864 Size: 423 34 227 103 77 Fragments arranged by size: 423 227 103 77 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 688 820 864 Size: 688 132 44 Fragments arranged by size: 688 132 44 EcoO109I rG'GnC_Cy Cuts at: 0 299 864 Size: 299 565 HaeII r_GCGC'y Cuts at: 0 541 864 Size: 541 323 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 348 380 578 610 864 Size: 348 32 198 32 254 Fragments arranged by size: 348 254 198 32 32 HincII GTy'rAC Cuts at: 0 414 812 864 Size: 414 398 52 Fragments arranged by size: 414 398 52 KpnI G_GTAC'C Cuts at: 0 135 864 Size: 135 729 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 544 864 Size: 544 320 MscI TGG'CCA Cuts at: 0 346 864 Size: 346 518 MslI CAynn'nnrTG Cuts at: 0 640 864 Size: 640 224 MspA1I CmG'CkG Cuts at: 0 6 435 864 Size: 6 429 429 Fragments arranged by size: 429 429 6 PasI CC'CwG_GG Cuts at: 0 816 864 Size: 816 48 PpuMI rG'GwC_Cy Cuts at: 0 299 864 Size: 299 565 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 76 108 864 Size: 76 32 756 Fragments arranged by size: 756 76 32 PstI C_TGCA'G Cuts at: 0 692 864 Size: 692 172 PvuII CAG'CTG Cuts at: 0 435 864 Size: 435 429 SbfI CC_TGCA'GG Cuts at: 0 692 864 Size: 692 172 SexAI A'CCwGG_T Cuts at: 0 224 864 Size: 224 640 SfcI C'TryA_G Cuts at: 0 688 864 Size: 688 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 172 411 864 Size: 172 239 453 Fragments arranged by size: 453 239 172 TatI w'GTAC_w Cuts at: 0 230 350 518 864 Size: 230 120 168 346 Fragments arranged by size: 346 230 168 120 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 430 462 844 864 Size: 430 32 382 20 Fragments arranged by size: 430 382 32 20 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 838 864 Size: 838 26 Enzymes that do cut: AccI Acc65I AcuI AhdI AleI AlwNI AvaI BanI BanII BbsI BciVI Bme1580I BmgBI BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BsrFI BsrGI BstEII BstZ17I BtgI BtgZI EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I PasI PpuMI PsrI PstI PvuII SbfI SexAI SfcI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI