################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AlwI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HincII HindII HindIII HpaI KpnI MamI MflI MluI MroI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcsI 924 AAATTT [GA]^AATT[CT] AflIII 698 ACATGT A^C[GA][CT]GT AocI 435 CCTCAGG CC^T[ACGT]AGG ApoI 924 AAATTT [GA]^AATT[CT] AvaI 67 CCCGGG C^[CT]CG[GA]G BalI 404 TGGCCA TGG^CCA BanI 310 GGCGCC G^G[CT][GA]CC BbeI 310 GGCGCC GGCGC^C BbsI 616 GTCTTC GAAGAC(2/6) BbvCI 21 CCTCAGC CCTCAGC(-5/-2) BclI 161 TGATCA T^GATCA BfaI 157 CTAG C^TAG BglI 238 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 616 GTCTTC GAAGAC(2/6) BsgI 734 CTGCAC GTGCAG(16/14) BsiEI 11 CGGTCG CG[GA][CT]^CG BsmFI 444 GTCCC GGGAC(10/14) BsoBI 67 CCCGGG C^[CT]CG[GA]G BspLU11I 698 ACATGT A^CATGT BsrBI 634 CCGCTC CCGCTC(-3/-3) BsrDI 489 CATTGC GCAATG(2/0) BsrFI 834 GCCGGC [GA]^CCGG[CT] BstXI 488 CCATTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 435 CCTCAGG CC^T[ACGT]AGG Cfr10I 834 GCCGGC [GA]^CCGG[CT] Eco81I 435 CCTCAGG CC^T[ACGT]AGG EcoT22I 908 ATGCAT ATGCA^T FbaI 161 TGATCA T^GATCA HgaI 831 GACGC GACGC(5/10) KasI 310 GGCGCC G^GCGCC KspI 196 CCGCGG CCGC^GG MaeI 157 CTAG C^TAG MfeI 1032 CAATTG C^AATTG MluNI 404 TGGCCA TGG^CCA MscI 404 TGGCCA TGG^CCA MseI 671 TTAA T^TAA MunI 1032 CAATTG C^AATTG NaeI 834 GCCGGC GCC^GGC NarI 310 GGCGCC GG^CGCC NcoI 494 CCATGG C^CATGG NgoMIV 834 GCCGGC G^CCGGC NsiI 908 ATGCAT ATGCA^T NspI 698 ACATGT [GA]CATG^[CT] PflMI 864 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 665 AGGTCCT [GA]G^G[AT]CC[CT] SacII 196 CCGCGG CCGC^GG SapI 255 GCTCTTC GCTCTTC(1/4) SfoI 310 GGCGCC GGC^GCC SmaI 67 CCCGGG CCC^GGG TaqI 1008 TCGA T^CGA Tru9I 671 TTAA T^TAA TthHB8I 1008 TCGA T^CGA Van91I 864 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 296 CCACCTGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 67 CCCGGG C^CCGGG XmaI 67 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 310 GGCGCC G[GA]^CG[CT]C 831 GACGCC AluI 5 AGCT AG^CT 40 AGCT Alw44I 412 GTGCAC G^TGCAC 606 GTGCAC ApaLI 412 GTGCAC G^TGCAC 606 GTGCAC AvaII 393 GGACC G^G[AT]CC 666 GGTCC BanII 49 GAGCCC G[GA]GC[CT]^C 64 GAGCCC BsaAI 400 TACGTG [CT]AC^GT[GA] 598 TACGTG BsaHI 310 GGCGCC G[GA]^CG[CT]C 831 GACGCC BseRI 426 CTCCTC GAGGAG(10/8) 429 CTCCTC BspMI 251 ACCTGC ACCTGC(4/8) 626 ACCTGC BssHII 175 GCGCGC G^CGCGC 537 GCGCGC DpnII 126 GATC ^GATC 162 GATC DraII 521 AGGGCCT [GA]G^G[ACGT]CC[CT] 665 AGGTCCT EcoNI 21 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 431 CCTCCCTCAGG EcoO109I 521 AGGGCCT [GA]G^G[ACGT]CC[CT] 665 AGGTCCT EcoT14I 494 CCATGG C^C[AT][AT]GG 662 CCAAGG Hin1I 310 GGCGCC G[GA]^CG[CT]C 831 GACGCC MaeII 401 ACGT A^CGT 599 ACGT MaeIII 103 GTCAC ^GT[ACGT]AC 959 GTCAC MboI 126 GATC ^GATC 162 GATC NdeII 126 GATC ^GATC 162 GATC Sau3AI 126 GATC ^GATC 162 GATC StuI 593 AGGCCT AGG^CCT 935 AGGCCT StyI 494 CCATGG C^C[AT][AT]GG 662 CCAAGG Tsp45I 103 GTCAC ^GT[GC]AC 959 GTCAC TspRI 568 CAGTG CA[GC]TG[ACGT][ACGT]^ 865 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AspHI BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TfiI TseI Tsp509I