(Circular) (Six-base) MAPSORT of: pFN21AE9554.seq Check: 9819 from: 1 to: 5752 pFN21AE9554 5752 bp With 182 enzymes: SgfI * March 28, 2012 12:01 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2739 2839 3815 5199 5467 1632 Size: 1107 100 976 1384 268 1917 Fragments arranged by size: 1917 1384 1107 976 268 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 2703 4651 5628 1203 Size: 1500 1948 977 1327 Fragments arranged by size: 1948 1500 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 2114 2306 2988 1166 Size: 948 192 682 3930 Fragments arranged by size: 3930 948 682 192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2477 1184 Size: 1293 4459 AlwNI CAG_nnn'CTG Cuts at: 3495 5067 3495 Size: 1572 4180 ApaLI G'TGCA_C Cuts at: 2417 2611 3809 4965 2417 Size: 194 1198 1156 3204 Fragments arranged by size: 3204 1198 1156 194 ApoI r'AATT_y Cuts at: 1493 2929 3061 3287 3439 5548 1493 Size: 1436 132 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 226 152 132 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2072 3077 1870 Size: 73 129 1005 4545 Fragments arranged by size: 4545 1005 129 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 3073 4528 618 Size: 325 217 627 528 758 1455 1842 Fragments arranged by size: 1842 1455 758 627 528 325 217 BanII G_rGCy'C Cuts at: 1391 2058 2073 3071 1391 Size: 667 15 998 4072 Fragments arranged by size: 4072 998 667 15 BbeI G_GCGC'C Cuts at: 2319 2319 Size: 5752 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2614 928 Size: 971 715 4066 Fragments arranged by size: 4066 971 715 BbvCI CC'TCA_GC Cuts at: 2027 2027 Size: 5752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 2166 1548 Size: 618 5134 BfrBI ATG'CAT Cuts at: 2915 2915 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2249 4363 136 Size: 107 122 71 631 605 577 2114 1525 Fragments arranged by size: 2114 1525 631 605 577 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2421 2615 3813 4969 1790 Size: 631 194 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 631 194 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 4441 442 Size: 416 53 83 399 24 2066 958 1753 Fragments arranged by size: 2066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4412 1239 Size: 3173 2579 Bpu10I CC'TnA_GC Cuts at: 2027 2234 2027 Size: 207 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2407 2605 3543 493 Size: 711 1203 198 938 2702 Fragments arranged by size: 2702 1203 938 711 198 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2837 3559 3941 Size: 53 83 186 936 783 521 722 382 Cuts at: 3941 4576 275 Size: 635 1451 Fragments arranged by size: 1451 936 783 722 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5527 Fragments arranged by size: 5527 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2422 2425 1186 Size: 1236 3 4513 Fragments arranged by size: 4513 1236 3 BseYI C'CCAG_C Cuts at: 2140 2149 2311 2464 2565 4955 5451 2140 Size: 9 162 153 101 2390 496 2441 Fragments arranged by size: 2441 2390 496 162 153 101 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2724 1163 Size: 573 988 4191 Fragments arranged by size: 4191 988 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 3202 3963 4991 1540 Size: 275 186 18 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2210 2421 2615 3813 3898 4969 2210 Size: 211 194 1198 85 1071 2993 Fragments arranged by size: 2993 1198 1071 211 194 85 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2073 2210 2421 2615 3071 3813 Size: 399 268 15 137 211 194 456 742 Cuts at: 3813 3898 4969 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 268 211 194 137 85 15 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 2640 3107 844 Size: 1421 375 467 3489 Fragments arranged by size: 3489 1421 467 375 BsrBI CCG'CTC Cuts at: 1394 1823 2641 3639 1394 Size: 429 818 998 3507 Fragments arranged by size: 3507 998 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 4247 4421 65 Size: 1183 1245 1754 174 1396 Fragments arranged by size: 1754 1396 1245 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2839 4396 4583 1700 Size: 110 1029 1557 187 2869 Fragments arranged by size: 2869 1557 1029 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 2180 2542 1925 Size: 255 362 5135 Fragments arranged by size: 5135 362 255 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2500 2011 Size: 489 5263 BstYI r'GATC_y Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747 Size: 1110 367 385 17 1452 86 12 346 Cuts at: 5747 1972 Size: 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2441 2441 Size: 5752 BtgI C'CryG_G Cuts at: 513 1065 2004 2201 2394 2499 2511 513 Size: 552 939 197 193 105 12 3754 Fragments arranged by size: 3754 939 552 197 193 105 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2224 2402 487 Size: 683 56 998 178 3837 Fragments arranged by size: 3837 998 683 178 56 BtsI GCAGTG_nn' Cuts at: 2579 2869 3268 4062 4082 2579 Size: 290 399 794 20 4249 Fragments arranged by size: 4249 794 399 290 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2382 2409 Size: 54 1425 50 275 18 218 334 27 Cuts at: 2409 3199 4088 5717 8 Size: 790 889 1629 43 Fragments arranged by size: 1629 1425 889 790 334 275 218 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 2537 2858 3682 1353 Size: 914 270 321 824 3423 Fragments arranged by size: 3423 914 824 321 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2739 2839 3815 4412 5199 5467 1239 Size: 393 1107 100 976 597 787 268 1524 Fragments arranged by size: 1524 1107 976 787 597 393 268 100 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2440 2030 Size: 410 5342 EcoO109I rG'GnC_Cy Cuts at: 2527 2671 2527 Size: 144 5608 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 2319 2431 2489 4899 1678 Size: 641 112 58 2410 2531 Fragments arranged by size: 2531 2410 641 112 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373 Size: 32 135 32 2821 32 42 32 2626 Fragments arranged by size: 2821 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KasI G'GCGC_C Cuts at: 2315 2315 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3037 3351 3037 Size: 314 5438 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4866 5050 1305 Size: 3561 184 2007 Fragments arranged by size: 3561 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 10 Size: 54 1425 343 579 3351 Fragments arranged by size: 3351 1425 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2114 2306 2363 2498 2988 3710 Size: 648 927 21 192 57 135 490 722 Cuts at: 3710 4069 4228 518 Size: 359 159 2042 Fragments arranged by size: 2042 927 722 648 490 359 192 159 135 57 21 MspA1I CmG'CkG Cuts at: 1606 2080 2203 2833 3168 3845 4993 5238 1606 Size: 474 123 630 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 474 335 245 123 NaeI GCC'GGC Cuts at: 2841 2841 Size: 5752 NarI GG'CG_CC Cuts at: 2316 2316 Size: 5752 NcoI C'CATG_G Cuts at: 513 1065 2004 2499 513 Size: 552 939 495 3766 Fragments arranged by size: 3766 939 552 495 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NgoMIV G'CCGG_C Cuts at: 2839 2839 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NsiI A_TGCA'T Cuts at: 2917 2917 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 2707 3110 4655 1132 Size: 97 1478 403 1545 2229 Fragments arranged by size: 2229 1545 1478 403 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 2703 4651 2703 Size: 1948 3804 PflMI CCAn_nnn'nTGG Cuts at: 1108 2875 1108 Size: 1767 3985 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2671 2671 Size: 5752 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 SacII CC_GC'GG Cuts at: 2204 2204 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 5752 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 3100 4235 4916 5107 826 Size: 219 2055 1135 681 191 1471 Fragments arranged by size: 2055 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2317 2317 Size: 5752 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 2074 3079 1872 Size: 202 1005 4545 Fragments arranged by size: 4545 1005 202 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SrfI GCCC'GGGC Cuts at: 2074 2074 Size: 5752 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 2600 2942 1517 Size: 1083 342 4327 Fragments arranged by size: 4327 1083 342 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 2667 3191 513 Size: 552 939 495 168 524 3074 Fragments arranged by size: 3074 939 552 524 495 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2296 3779 3796 3949 4134 4579 325 Size: 1255 716 1483 17 153 185 445 1498 Fragments arranged by size: 1498 1483 1255 716 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3041 3998 5710 Size: 274 80 33 51 1443 1064 957 1712 Cuts at: 5710 96 Size: 138 Fragments arranged by size: 1712 1443 1064 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 2308 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 2072 3077 1870 Size: 202 1005 4545 Fragments arranged by size: 4545 1005 202 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SwaI