(Linear) (Six-base) MAPSORT of: KIEE9554.seq Check: 8373 from: 1 to: 1047 KIEE9554.orf With 182 enzymes: SgfI * March 22, 2012 18:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 734 834 1047 Size: 734 100 213 Fragments arranged by size: 734 213 100 AflIII A'CryG_T Cuts at: 0 698 1047 Size: 698 349 AleI CACnn'nnGTG Cuts at: 0 109 301 983 1047 Size: 109 192 682 64 Fragments arranged by size: 682 192 109 64 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 472 1047 Size: 472 575 ApaLI G'TGCA_C Cuts at: 0 412 606 1047 Size: 412 194 441 Fragments arranged by size: 441 412 194 ApoI r'AATT_y Cuts at: 0 924 1047 Size: 924 123 AvaI C'yCGr_G Cuts at: 0 67 1047 Size: 67 980 BanI G'GyrC_C Cuts at: 0 310 1047 Size: 310 737 BanII G_rGCy'C Cuts at: 0 53 68 1047 Size: 53 15 979 Fragments arranged by size: 979 53 15 BbeI G_GCGC'C Cuts at: 0 314 1047 Size: 314 733 BbsI GAAGACnn'nnnn_ Cuts at: 0 609 1047 Size: 609 438 BbvCI CC'TCA_GC Cuts at: 0 22 1047 Size: 22 1025 BclI T'GATC_A Cuts at: 0 161 1047 Size: 161 886 BfrBI ATG'CAT Cuts at: 0 910 1047 Size: 910 137 BglI GCCn_nnn'nGGC Cuts at: 0 244 1047 Size: 244 803 Bme1580I G_kGCm'C Cuts at: 0 416 610 1047 Size: 416 194 437 Fragments arranged by size: 437 416 194 Bpu10I CC'TnA_GC Cuts at: 0 22 229 1047 Size: 22 207 818 Fragments arranged by size: 818 207 22 BsaAI yAC'GTr Cuts at: 0 402 600 1047 Size: 402 198 447 Fragments arranged by size: 447 402 198 BsaHI Gr'CG_yC Cuts at: 0 311 832 1047 Size: 311 521 215 Fragments arranged by size: 521 311 215 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 417 420 1047 Size: 417 3 627 Fragments arranged by size: 627 417 3 BseYI C'CCAG_C Cuts at: 0 135 144 306 459 560 1047 Size: 135 9 162 153 101 487 Fragments arranged by size: 487 162 153 135 101 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 719 1047 Size: 719 328 BsiEI CG_ry'CG Cuts at: 0 14 1047 Size: 14 1033 BsiHKAI G_wGCw'C Cuts at: 0 205 416 610 1047 Size: 205 211 194 437 Fragments arranged by size: 437 211 205 194 Bsp1286I G_dGCh'C Cuts at: 0 53 68 205 416 610 1047 Size: 53 15 137 211 194 437 Fragments arranged by size: 437 211 194 137 53 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 260 635 1047 Size: 260 375 412 Fragments arranged by size: 412 375 260 BsrBI CCG'CTC Cuts at: 0 636 1047 Size: 636 411 BsrDI GCAATG_nn' Cuts at: 0 488 1047 Size: 488 559 BsrFI r'CCGG_y Cuts at: 0 834 1047 Size: 834 213 BssHII G'CGCG_C Cuts at: 0 175 537 1047 Size: 175 362 510 Fragments arranged by size: 510 362 175 BstXI CCAn_nnnn'nTGG Cuts at: 0 495 1047 Size: 495 552 Bsu36I CC'TnA_GG Cuts at: 0 436 1047 Size: 436 611 BtgI C'CryG_G Cuts at: 0 196 389 494 506 1047 Size: 196 193 105 12 541 Fragments arranged by size: 541 196 193 105 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 219 397 1047 Size: 219 178 650 Fragments arranged by size: 650 219 178 BtsI GCAGTG_nn' Cuts at: 0 574 864 1047 Size: 574 290 183 Fragments arranged by size: 574 290 183 EaeI y'GGCC_r Cuts at: 0 43 377 404 1047 Size: 43 334 27 643 Fragments arranged by size: 643 334 43 27 EarI CTCTTCn'nnn_ Cuts at: 0 262 532 853 1047 Size: 262 270 321 194 Fragments arranged by size: 321 270 262 194 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 734 834 1047 Size: 734 100 213 Fragments arranged by size: 734 213 100 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 435 1047 Size: 25 410 612 Fragments arranged by size: 612 410 25 EcoO109I rG'GnC_Cy Cuts at: 0 522 666 1047 Size: 522 144 381 Fragments arranged by size: 522 381 144 HaeII r_GCGC'y Cuts at: 0 314 426 484 1047 Size: 314 112 58 563 Fragments arranged by size: 563 314 112 58 KasI G'GCGC_C Cuts at: 0 310 1047 Size: 310 737 MfeI C'AATT_G Cuts at: 0 1032 1047 Size: 1032 15 MscI TGG'CCA Cuts at: 0 406 1047 Size: 406 641 MslI CAynn'nnrTG Cuts at: 0 88 109 301 358 493 983 1047 Size: 88 21 192 57 135 490 64 Fragments arranged by size: 490 192 135 88 64 57 21 MspA1I CmG'CkG Cuts at: 0 75 198 828 1047 Size: 75 123 630 219 Fragments arranged by size: 630 219 123 75 NaeI GCC'GGC Cuts at: 0 836 1047 Size: 836 211 NarI GG'CG_CC Cuts at: 0 311 1047 Size: 311 736 NcoI C'CATG_G Cuts at: 0 494 1047 Size: 494 553 NgoMIV G'CCGG_C Cuts at: 0 834 1047 Size: 834 213 NsiI A_TGCA'T Cuts at: 0 912 1047 Size: 912 135 NspI r_CATG'y Cuts at: 0 702 1047 Size: 702 345 PciI A'CATG_T Cuts at: 0 698 1047 Size: 698 349 PflMI CCAn_nnn'nTGG Cuts at: 0 870 1047 Size: 870 177 PpuMI rG'GwC_Cy Cuts at: 0 666 1047 Size: 666 381 SacII CC_GC'GG Cuts at: 0 199 1047 Size: 199 848 SapI GCTCTTCn'nnn_ Cuts at: 0 262 1047 Size: 262 785 SfoI GGC'GCC Cuts at: 0 312 1047 Size: 312 735 SmaI CCC'GGG Cuts at: 0 69 1047 Size: 69 978 SrfI GCCC'GGGC Cuts at: 0 69 1047 Size: 69 978 StuI AGG'CCT Cuts at: 0 595 937 1047 Size: 595 342 110 Fragments arranged by size: 595 342 110 StyI C'CwwG_G Cuts at: 0 494 662 1047 Size: 494 168 385 Fragments arranged by size: 494 385 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 291 1047 Size: 291 756 TatI w'GTAC_w Cuts at: 0 1036 1047 Size: 1036 11 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 303 1047 Size: 303 744 XmaI C'CCGG_G Cuts at: 0 67 1047 Size: 67 980 Enzymes that do cut: AcuI AflIII AleI AlfI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BclI BfrBI BglI Bme1580I Bpu10I BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstXI Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I HaeII KasI MfeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PpuMI SacII SapI SfoI SmaI SrfI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BglII BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MluI MmeI NdeI NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI