################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI TseI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcsI 160 AAATTT [GA]^AATT[CT] AflIII 927 ACATGT A^C[GA][CT]GT Alw44I 1195 GTGCAC G^TGCAC AlwNI 440 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1195 GTGCAC G^TGCAC ApoI 160 AAATTT [GA]^AATT[CT] AvaI 811 CCCGAG C^[CT]CG[GA]G AvaII 1251 GGTCC G^G[AT]CC BalI 469 TGGCCA TGG^CCA BbrPI 1198 CACGTG CAC^GTG BcnI 1101 CCCGG CC^[GC]GG BsaAI 1198 CACGTG [CT]AC^GT[GA] BsaBI 114 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1222 GAGACC GGTCTC(1/5) BseRI 741 GAGGAG GAGGAG(10/8) BsmAI 1222 GAGAC GTCTC(1/5) BsmI 567 GCATTC GAATGC(1/-1) BsoBI 811 CCCGAG C^[CT]CG[GA]G BspLU11I 927 ACATGT A^CATGT BspMI 141 ACCTGC ACCTGC(4/8) BssSI 999 CTCGTG CACGAG(-5/-1) BstXI 1281 CCATTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 641 GGATCT [GA]^GATC[CT] CfoI 78 GCGC GCG^C DraI 951 TTTAAA TTT^AAA DraII 338 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 730 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 469 TGGCCA [CT]^GGCC[GA] EcoNI 307 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 338 AGGCCCT [GA]G^G[ACGT]CC[CT] Fnu4HI 274 GCCGC GC^[ACGT]GC HaeII 77 GGCGCT [GA]GCGC^[CT] HapII 1102 CCGG C^CGG HhaI 78 GCGC GCG^C HinP1I 78 GCGC G^CGC HpaII 1102 CCGG C^CGG ItaI 274 GCCGC GC^[ACGT]GC MamI 114 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 641 GGATCT [GA]^GATC[CT] MluNI 469 TGGCCA TGG^CCA MscI 469 TGGCCA TGG^CCA MslI 853 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 855 CAGCGG C[AC]G^C[GT]G MspI 1102 CCGG C^CGG NciI 1101 CCCGG CC^[GC]GG PmaCI 1198 CACGTG CAC^GTG PmlI 1198 CACGTG CAC^GTG SexAI 1247 ACCTGGT A^CC[AT]GGT StuI 315 AGGCCT AGG^CCT TfiI 119 GAATC G^A[AT]TC Tsp45I 626 GTGAC ^GT[GC]AC XhoII 641 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AspHI 744 GAGCAC G[AT]GC[AT]^C 1195 GTGCAC BmyI 744 GAGCAC G[AGT]GC[ACT]^C 1195 GTGCAC BsaJI 657 CCCTGG C^C[ACGT][ACGT]GG 1100 CCCCGG BsiHKAI 744 GAGCAC G[AT]GC[AT]^C 1195 GTGCAC BsmFI 1152 GGGAC GGGAC(10/14) 1291 GGGAC Bsp1286I 744 GAGCAC G[AGT]GC[ACT]^C 1195 GTGCAC Cfr13I 339 GGCCC G^G[ACGT]CC 1251 GGTCC EarI 895 CTCTTC CTCTTC(1/4) 1190 GAAGAG Ksp632I 895 CTCTTC CTCTTC(1/4) 1190 GAAGAG NspI 411 GCATGT [GA]CATG^[CT] 927 ACATGT Sau96I 339 GGCCC G^G[ACGT]CC 1251 GGTCC SspI 348 AATATT AAT^ATT 913 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AfaI AluI AlwI BfaI BsgI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI HaeIII HincII HindII HinfI HphI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfaNI Tru9I Tsp509I