(Circular) (Six-base) MAPSORT of: pFN21AE3973.seq Check: 8438 from: 1 to: 6007 pFN21AE3973 6007 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2155 2155 Size: 6007 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 2045 4070 5454 5722 1632 Size: 363 50 2025 1384 268 1917 Fragments arranged by size: 2025 1917 1384 363 268 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 2932 4906 5883 1203 Size: 1729 1974 977 1327 Fragments arranged by size: 1974 1729 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 2862 1166 Size: 1696 4311 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 2450 3750 5322 2450 Size: 1300 1572 3135 Fragments arranged by size: 3135 1572 1300 ApaLI G'TGCA_C Cuts at: 3200 4064 5220 3200 Size: 864 1156 3987 Fragments arranged by size: 3987 1156 864 ApoI r'AATT_y Cuts at: 1493 2165 3316 3542 3694 5803 1493 Size: 672 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 672 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2816 3332 1870 Size: 73 873 516 4545 Fragments arranged by size: 4545 873 516 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3328 4783 618 Size: 325 217 627 1541 1455 1842 Fragments arranged by size: 1842 1541 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3326 1391 Size: 1935 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5036 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4198 4232 1903 Size: 34 50 34 2177 34 3678 Fragments arranged by size: 3678 2177 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6002 6002 Size: 6007 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 3204 4068 5224 1790 Size: 1414 864 1156 2573 Fragments arranged by size: 2573 1414 1156 864 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3738 4696 442 Size: 416 53 83 399 24 2321 958 1753 Fragments arranged by size: 2321 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4667 1239 Size: 3428 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3221 4670 882 Size: 617 1722 1449 2219 Fragments arranged by size: 2219 1722 1449 617 BsaAI yAC'GTr Cuts at: 493 1204 3205 3798 493 Size: 711 2001 593 2702 Fragments arranged by size: 2702 2001 711 593 BsaBI GATnn'nnATC Cuts at: 2123 6001 2123 Size: 3878 2129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3814 4196 4831 275 Size: 53 83 186 936 2281 382 635 1451 Fragments arranged by size: 2281 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2547 2577 3200 3230 1178 Size: 30 165 30 1144 30 623 30 3955 Fragments arranged by size: 3955 1144 623 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2761 1186 Size: 1575 4432 BseYI C'CCAG_C Cuts at: 5210 5706 5210 Size: 496 5511 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 2813 2840 1163 Size: 573 906 171 27 4330 Fragments arranged by size: 4330 906 573 171 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2753 3204 4068 4153 5224 2753 Size: 451 864 85 1071 3536 Fragments arranged by size: 3536 1071 864 451 85 BsmI GAATG_Cn' Cuts at: 2572 3518 3611 2572 Size: 946 93 4968 Fragments arranged by size: 4968 946 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2753 3204 3326 4068 4153 5224 1391 Size: 399 963 451 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 963 742 451 399 122 85 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2155 3362 844 Size: 1311 1207 3489 Fragments arranged by size: 3489 1311 1207 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4651 4838 1700 Size: 110 2841 187 2869 Fragments arranged by size: 2869 2841 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 3004 4067 5079 3004 Size: 1063 1012 3932 Fragments arranged by size: 3932 1063 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6007 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 3293 3293 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2646 3337 3704 4089 4106 5558 5644 5656 Size: 674 691 367 385 17 1452 86 12 Cuts at: 5656 6002 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 691 674 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5455 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 2958 3311 2958 Size: 353 5654 DraIII CAC_nnn'GTG Cuts at: 2740 2740 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2474 3454 4343 Size: 54 1425 50 275 18 644 980 889 Cuts at: 4343 5972 8 Size: 1629 43 Fragments arranged by size: 1629 1425 980 889 644 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2906 3190 3937 1353 Size: 1553 284 747 3423 Fragments arranged by size: 3423 1553 747 284 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2045 4070 4667 5454 5722 1239 Size: 393 363 50 2025 597 787 268 1524 Fragments arranged by size: 2025 1524 787 597 393 363 268 50 EcoNI CCTnn'n_nnAGG Cuts at: 2316 2316 Size: 6007 EcoO109I rG'GnC_Cy Cuts at: 2344 2344 Size: 6007 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3257 3257 Size: 6007 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2086 5154 1678 Size: 408 3068 2531 Fragments arranged by size: 3068 2531 408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373 Size: 32 135 32 3076 32 42 32 2626 Fragments arranged by size: 3076 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 2496 3250 3351 3597 669 Size: 988 281 370 188 754 101 246 3079 Fragments arranged by size: 3079 988 754 370 281 246 188 101 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5121 5305 1305 Size: 3816 184 2007 Fragments arranged by size: 3816 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2476 10 Size: 54 1425 343 644 3541 Fragments arranged by size: 3541 1425 644 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2862 3965 4324 4483 518 Size: 648 1696 1103 359 159 2042 Fragments arranged by size: 2042 1696 1103 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2862 3423 4100 5248 5493 1606 Size: 1256 561 677 1148 245 2120 Fragments arranged by size: 2120 1256 1148 677 561 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5455 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2420 2936 3365 4910 1132 Size: 97 1191 516 429 1545 2229 Fragments arranged by size: 2229 1545 1191 516 429 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 2932 4906 2932 Size: 1974 4033 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PmlI CAC'GTG Cuts at: 3205 3205 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 3359 830 Size: 2529 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SexAI A'CCwGG_T Cuts at: 3252 3252 Size: 6007 SfcI C'TryA_G Cuts at: 826 1045 3355 4490 5171 5362 826 Size: 219 2310 1135 681 191 1471 Fragments arranged by size: 2310 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4111 5012 5274 820 Size: 197 926 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 2355 2920 3931 5 Size: 47 2303 565 1011 2081 Fragments arranged by size: 2303 2081 1011 565 47 StuI AGG'CCT Cuts at: 1517 2322 1517 Size: 805 5202 StyI C'CwwG_G Cuts at: 513 1065 3446 513 Size: 552 2381 3074 Fragments arranged by size: 3074 2381 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3245 4034 4051 4204 4389 4834 325 Size: 1255 1665 789 17 153 185 445 1498 Fragments arranged by size: 1665 1498 1255 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2063 2589 4253 Size: 274 80 33 51 1443 86 526 1664 Cuts at: 4253 5965 96 Size: 1712 138 Fragments arranged by size: 1712 1664 1443 526 274 138 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2308 2340 3200 3232 5967 5999 2308 Size: 32 860 32 2735 32 2316 Fragments arranged by size: 2735 2316 860 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI