(Circular) (Six-base) MAPSORT of: pFN21AE1823.seq Check: 6011 from: 1 to: 5713 pFN21AE1823 5713 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2043 3056 1937 Size: 106 1013 4594 Fragments arranged by size: 4594 1013 106 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2322 3776 5160 5428 1632 Size: 690 1454 1384 268 1917 Fragments arranged by size: 1917 1454 1384 690 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 2100 4612 5589 1203 Size: 897 2512 977 1327 Fragments arranged by size: 2512 1327 977 897 AgeI A'CCGG_T Cuts at: 2921 4544 2921 Size: 1623 4090 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5713 AlwNI CAG_nnn'CTG Cuts at: 3456 5028 3456 Size: 1572 4141 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 2251 2560 3022 3248 3400 5509 1493 Size: 758 309 462 226 152 2109 1697 Fragments arranged by size: 2109 1697 758 462 309 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 2653 3038 1870 Size: 73 710 385 4545 Fragments arranged by size: 4545 710 385 73 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2890 3034 4489 618 Size: 325 217 627 1103 144 1455 1842 Fragments arranged by size: 1842 1455 1103 627 325 217 144 BanII G_rGCy'C Cuts at: 1391 2053 3032 1391 Size: 662 979 4072 Fragments arranged by size: 4072 979 662 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2130 2872 3605 4815 2130 Size: 742 733 1210 3028 Fragments arranged by size: 3028 1210 742 733 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4324 136 Size: 107 122 71 631 605 2652 1525 Fragments arranged by size: 2652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2857 5708 2857 Size: 2851 2862 BlpI GC'TnA_GC Cuts at: 3130 3130 Size: 5713 Bme1580I G_kGCm'C Cuts at: 1790 3774 4930 1790 Size: 1984 1156 2573 Fragments arranged by size: 2573 1984 1156 BmgBI CAC'GTC Cuts at: 1318 2475 1318 Size: 1157 4556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3444 4402 442 Size: 416 53 83 399 24 2027 958 1753 Fragments arranged by size: 2027 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2787 4373 1239 Size: 1548 1586 2579 Fragments arranged by size: 2579 1586 1548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3838 4703 5001 3838 Size: 865 298 4550 Fragments arranged by size: 4550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2921 4146 4544 4818 4965 5376 2921 Size: 1225 398 274 147 411 3258 Fragments arranged by size: 3258 1225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2760 2790 1178 Size: 30 165 30 1357 30 4101 Fragments arranged by size: 4101 1357 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5713 BseYI C'CCAG_C Cuts at: 2169 4916 5412 2169 Size: 2747 496 2470 Fragments arranged by size: 2747 2470 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 1163 Size: 573 768 4372 Fragments arranged by size: 4372 768 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3163 3924 4952 1540 Size: 275 186 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2053 3774 3859 4930 2053 Size: 1721 85 1071 2836 Fragments arranged by size: 2836 1721 1071 85 BsmI GAATG_Cn' Cuts at: 2299 3224 3317 2299 Size: 925 93 4695 Fragments arranged by size: 4695 925 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 3032 3774 3859 4930 1391 Size: 399 263 979 742 85 1071 2174 Fragments arranged by size: 2174 1071 979 742 399 263 85 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 3602 5332 3602 Size: 1730 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3068 844 Size: 2224 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3600 1394 Size: 429 1777 3507 Fragments arranged by size: 3507 1777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2921 4357 4544 1700 Size: 110 1111 1436 187 2869 Fragments arranged by size: 2869 1436 1111 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5713 BstBI TT'CG_AA Cuts at: 4517 4517 Size: 5713 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstYI r'GATC_y Cuts at: 1972 2505 2857 3043 3410 3795 3812 5264 5350 Size: 533 352 186 367 385 17 1452 86 Cuts at: 5350 5362 5708 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 533 385 367 352 346 186 86 17 12 BstZ17I GTA'TAC Cuts at: 2044 2044 Size: 5713 BtgI C'CryG_G Cuts at: 513 1065 2004 2551 2605 2943 513 Size: 552 939 547 54 338 3283 Fragments arranged by size: 3283 939 552 547 338 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 2496 3229 4023 4043 2496 Size: 733 794 20 4166 Fragments arranged by size: 4166 794 733 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2797 2832 529 Size: 35 2233 35 3410 Fragments arranged by size: 3410 2233 35 35 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3160 4049 5678 Size: 54 1425 50 275 18 1330 889 1629 Cuts at: 5678 8 Size: 43 Fragments arranged by size: 1629 1425 1330 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3160 1537 Size: 275 1348 4090 Fragments arranged by size: 4090 1348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 2055 2848 3643 1353 Size: 684 18 793 795 3423 Fragments arranged by size: 3423 795 793 684 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2051 2051 Size: 5713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2322 2787 3776 4373 5160 5428 1239 Size: 393 690 465 989 597 787 268 1524 Fragments arranged by size: 1524 989 787 690 597 465 393 268 EcoO109I rG'GnC_Cy Cuts at: 2166 2166 Size: 5713 EcoRI G'AATT_C Cuts at: 2251 3022 3400 2251 Size: 771 378 4564 Fragments arranged by size: 4564 771 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2084 2084 Size: 5713 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2842 2874 4354 Size: 32 135 32 515 32 723 32 1480 Cuts at: 4354 4386 4428 4460 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1480 723 515 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2927 2974 3057 3303 669 Size: 988 281 989 47 83 246 3079 Fragments arranged by size: 3079 989 988 281 246 83 47 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3005 4827 5011 1305 Size: 1700 1822 184 2007 Fragments arranged by size: 2007 1822 1700 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3891 Fragments arranged by size: 3891 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3671 4030 4189 518 Size: 648 2505 359 159 2042 Fragments arranged by size: 2505 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2667 3129 3806 4954 5199 1606 Size: 796 265 462 677 1148 245 2120 Fragments arranged by size: 2120 1148 796 677 462 265 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2551 2943 513 Size: 552 939 547 392 3283 Fragments arranged by size: 3283 939 552 547 392 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 2104 3071 4616 1132 Size: 97 875 967 1545 2229 Fragments arranged by size: 2229 1545 967 875 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5713 PciI A'CATG_T Cuts at: 2100 4612 2100 Size: 2512 3201 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5713 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2166 2166 Size: 5713 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 2603 3065 830 Size: 1773 462 3478 Fragments arranged by size: 3478 1773 462 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 PvuII CAG'CTG Cuts at: 2402 2402 Size: 5713 SacI G_AGCT'C Cuts at: 2053 2053 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SanDI GG'GwC_CC Cuts at: 2166 2166 Size: 5713 SapI GCTCTTCn'nnn_ Cuts at: 2037 2037 Size: 5713 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 2599 2721 2902 3061 4196 4877 5068 Size: 219 1554 122 181 159 1135 681 191 Cuts at: 5068 826 Size: 1471 Fragments arranged by size: 1554 1471 1135 681 219 191 181 159 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4718 4980 820 Size: 197 926 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5713 StyI C'CwwG_G Cuts at: 513 1065 2004 2551 2943 3152 513 Size: 552 939 547 392 209 3074 Fragments arranged by size: 3074 939 552 547 392 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2908 3740 3757 3910 4095 4540 325 Size: 1255 1328 832 17 153 185 445 1498 Fragments arranged by size: 1498 1328 1255 832 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3959 5671 96 Size: 274 80 33 51 1443 1982 1712 138 Fragments arranged by size: 1982 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 2107 3049 2107 Size: 942 4771 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2352 2941 2352 Size: 589 5124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 3842 3842 Size: 5713 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI