(Circular) (Six-base) MAPSORT of: pFN21AB6540.seq Check: 8118 from: 1 to: 5878 pFN21AB6540 5878 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2235 2914 3941 5325 5593 1632 Size: 449 154 679 1027 1384 268 1917 Fragments arranged by size: 1917 1384 1027 679 449 268 154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2756 1184 Size: 1572 4306 AlwNI CAG_nnn'CTG Cuts at: 3020 3621 5193 3020 Size: 601 1572 3705 Fragments arranged by size: 3705 1572 601 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2603 2697 3187 3413 3565 5674 1493 Size: 1110 94 490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1110 490 226 152 94 AseI AT'TA_AT Cuts at: 160 1032 2448 4432 160 Size: 872 1416 1984 1606 Fragments arranged by size: 1984 1606 1416 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2953 3203 1870 Size: 73 196 814 250 4545 Fragments arranged by size: 4545 814 250 196 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 3029 3199 4654 618 Size: 325 217 627 231 1011 170 1455 1842 Fragments arranged by size: 1842 1455 1011 627 325 231 217 170 BanII G_rGCy'C Cuts at: 1391 2201 3197 1391 Size: 810 996 4072 Fragments arranged by size: 4072 996 810 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 5878 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BfrBI ATG'CAT Cuts at: 2393 2393 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 3939 5095 1790 Size: 2149 1156 2573 Fragments arranged by size: 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2152 3609 4567 Size: 416 53 83 399 24 735 1457 958 Cuts at: 4567 442 Size: 1753 Fragments arranged by size: 1753 1457 958 735 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2699 2789 4538 1239 Size: 1460 90 1749 2579 Fragments arranged by size: 2579 1749 1460 90 Bpu10I CC'TnA_GC Cuts at: 2729 2729 Size: 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2185 4003 4868 5166 2185 Size: 1818 865 298 2897 Fragments arranged by size: 2897 1818 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 3685 4067 4702 Size: 53 83 186 936 486 1666 382 635 Cuts at: 4702 275 Size: 1451 Fragments arranged by size: 1666 1451 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2186 1178 Size: 30 165 30 753 30 4870 Fragments arranged by size: 4870 753 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2157 2180 1186 Size: 971 23 4884 Fragments arranged by size: 4884 971 23 BseYI C'CCAG_C Cuts at: 5081 5577 5081 Size: 496 5382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2837 2981 1163 Size: 573 1101 144 4060 Fragments arranged by size: 4060 1101 573 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2201 2624 2940 3939 4024 5095 2201 Size: 423 316 999 85 1071 2984 Fragments arranged by size: 2984 1071 999 423 316 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 BsmBI CGTCTCn'nnnn_ Cuts at: 2183 2183 Size: 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2624 2940 3197 3939 4024 5095 Size: 399 411 423 316 257 742 85 1071 Cuts at: 5095 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 423 411 399 316 257 85 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 3085 3767 5497 3085 Size: 682 1730 3466 Fragments arranged by size: 3466 1730 682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3233 844 Size: 2389 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2567 4373 4547 65 Size: 1183 1319 1806 174 1396 Fragments arranged by size: 1806 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 2635 96 Size: 2539 3339 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2917 2917 Size: 5878 BstYI r'GATC_y Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873 Size: 1236 367 385 17 1452 86 12 346 Cuts at: 5873 1972 Size: 1977 Fragments arranged by size: 1977 1452 1236 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 3481 Fragments arranged by size: 3481 939 906 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2937 487 Size: 683 56 1711 3428 Fragments arranged by size: 3428 1711 683 56 BtsI GCAGTG_nn' Cuts at: 3097 3394 4188 4208 3097 Size: 297 794 20 4767 Fragments arranged by size: 4767 794 297 20 ClaI AT'CG_AT Cuts at: 1554 2760 1554 Size: 1206 4672 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 2554 3182 2554 Size: 628 5250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3325 4214 5843 Size: 54 1425 50 275 18 1495 889 1629 Cuts at: 5843 8 Size: 43 Fragments arranged by size: 1629 1495 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 2354 2592 3158 3808 1353 Size: 836 165 238 566 650 3423 Fragments arranged by size: 3423 836 650 566 238 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3064 4465 4839 4985 387 Size: 1064 138 1475 1401 374 146 1280 Fragments arranged by size: 1475 1401 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2199 2199 Size: 5878 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2235 2699 2789 2914 3941 4538 Size: 393 449 154 464 90 125 1027 597 Cuts at: 4538 5325 5593 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1027 787 597 464 449 393 268 154 125 90 EcoO109I rG'GnC_Cy Cuts at: 2136 2136 Size: 5878 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2022 5025 1678 Size: 344 3003 2531 Fragments arranged by size: 3003 2531 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373 Size: 32 135 32 2947 32 42 32 2626 Fragments arranged by size: 2947 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KasI G'GCGC_C Cuts at: 2018 2018 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 2115 3477 2115 Size: 1362 4516 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 3165 4992 5176 1305 Size: 837 1023 1827 184 2007 Fragments arranged by size: 2007 1827 1023 837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 2915 3090 3836 4195 4354 518 Size: 648 1572 177 175 746 359 159 2042 Fragments arranged by size: 2042 1572 746 648 359 177 175 159 MspA1I CmG'CkG Cuts at: 1606 3044 3294 3971 5119 5364 1606 Size: 1438 250 677 1148 245 2120 Fragments arranged by size: 2120 1438 1148 677 250 245 NarI GG'CG_CC Cuts at: 2019 2019 Size: 5878 NcoI C'CATG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 3481 Fragments arranged by size: 3481 939 906 552 NdeI CA'TA_TG Cuts at: 387 2829 387 Size: 2442 3436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NsiI A_TGCA'T Cuts at: 2395 2395 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2737 3236 4781 1132 Size: 97 1508 499 1545 2229 Fragments arranged by size: 2229 1545 1508 499 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2676 2676 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2136 Size: 5878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 3043 3230 830 Size: 2213 187 3478 Fragments arranged by size: 3478 2213 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 3044 3044 Size: 5878 SacI G_AGCT'C Cuts at: 2201 2201 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SanDI GG'GwC_CC Cuts at: 2136 2136 Size: 5878 SapI GCTCTTCn'nnn_ Cuts at: 2189 2189 Size: 5878 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 3039 3226 4361 5042 5233 826 Size: 219 1994 187 1135 681 191 1471 Fragments arranged by size: 1994 1471 1135 681 219 191 187 SfoI GGC'GCC Cuts at: 2020 2020 Size: 5878 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2200 3982 4883 5145 820 Size: 197 926 257 1782 901 262 1553 Fragments arranged by size: 1782 1553 926 901 262 257 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 2816 152 Size: 2664 3214 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 2384 3802 5 Size: 47 2332 1418 2081 Fragments arranged by size: 2332 2081 1418 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2004 2910 3317 513 Size: 552 939 906 407 3074 Fragments arranged by size: 3074 939 906 552 407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3905 3922 4075 4260 4705 325 Size: 1255 2325 17 153 185 445 1498 Fragments arranged by size: 2325 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 2635 4124 Size: 274 80 33 51 1443 271 387 1489 Cuts at: 4124 5836 96 Size: 1712 138 Fragments arranged by size: 1712 1489 1443 387 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3122 3154 5838 5870 3122 Size: 32 2684 32 3130 Fragments arranged by size: 3130 2684 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 2890 3214 2890 Size: 324 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2530 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 3093 4007 3093 Size: 914 4964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI