################################## # NO CUT ENZYMES ( 221 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MnlI MroI MscI MseI MslI MspA1I MunI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### AluI 59 AGCT AG^CT BpmI 50 CTCCAG CTGGAG(16/14) BsaJI 4 CCTGGG C^C[ACGT][ACGT]GG BsiYI 39 CCCCGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 39 CCCCGCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 98 GAGAC GTCTC(1/5) BssHII 16 GCGCGC G^CGCGC BssKI 4 CCTGG ^CC[ACGT]GG BstNI 4 CCTGG CC^[AT]GG EarI 145 CTCTTC CTCTTC(1/4) EcoRII 4 CCTGG ^CC[AT]GG EcoRV 135 GATATC GAT^ATC HapII 114 CCGG C^CGG HinfI 129 GAATC G^A[ACGT]TC HpaII 114 CCGG C^CGG Ksp632I 145 CTCTTC CTCTTC(1/4) MspI 114 CCGG C^CGG MvaI 4 CCTGG CC^[AT]GG ScrFI 4 CCTGG CC^[ACGT]GG TaqI 68 TCGA T^CGA TfiI 129 GAATC G^A[AT]TC TthHB8I 68 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AciI 41 CCGC CCGC(-3/-1) 45 GCGG BanII 36 GAGCCC G[GA]GC[CT]^C 47 GGGCTC BmyI 36 GAGCCC G[AGT]GC[ACT]^C 47 GGGCTC Bsp1286I 36 GAGCCC G[AGT]GC[ACT]^C 47 GGGCTC Cac8I 16 GCGCGC GC[ACGT]^[ACGT]GC 45 GCGGGC DdeI 85 CTCAG C^T[ACGT]AG 153 CTCAG FauI 40 CCCGC CCCGC(4/6) 45 GCGGG MboII 9 GAAGA GAAGA(8/7) 146 TCTTC NlaIV 48 GGCTCC GG[ACGT]^[ACGT]CC 157 GGAACC ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AccII BstUI CfoI HhaI HinP1I MvnI