################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstYI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT14I EcoT22I FbaI Fnu4HI FokI FseI FspI HincII HindII HindIII HpaI ItaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TseI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 36 CGCG CG^CG AcyI 13 GGCGCC G[GA]^CG[CT]C AfaI 411 GTAC GT^AC AspHI 157 GTGCTC G[AT]GC[AT]^C AvaII 60 GGACC G^G[AT]CC BanI 13 GGCGCC G^G[CT][GA]CC BbeI 13 GGCGCC GGCGC^C BcnI 252 CCGGG CC^[GC]GG BglI 16 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 13 GGCGCC G[GA]^CG[CT]C BsaI 227 GAGACC GGTCTC(1/5) BsaJI 17 CCGTGG C^C[ACGT][ACGT]GG BseRI 224 GAGGAG GAGGAG(10/8) BsgI 128 CTGCAC GTGCAG(16/14) BsiHKAI 157 GTGCTC G[AT]GC[AT]^C BsmFI 59 GGGAC GGGAC(10/14) Bsp1407I 410 TGTACA T^GTACA BsrFI 210 GCCGGC [GA]^CCGG[CT] BsrGI 410 TGTACA T^GTACA BsrI 428 ACTGG ACTGG(1/-1) BstEII 489 GGTCACC G^GT[ACGT]ACC BstNI 96 CCAGG CC^[AT]GG BstPI 489 GGTCACC G^GT[ACGT]ACC BstUI 36 CGCG CG^CG BstXI 179 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 210 GCCGGC [GA]^CCGG[CT] Cfr13I 60 GGACC G^G[ACGT]CC DdeI 193 CTGAG C^T[ACGT]AG DraII 59 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 17 CCGTGG C^C[GA][CT]GG EarI 455 GAAGAG CTCTTC(1/4) EcoO109I 59 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 489 GGTCACC G^GT[ACGT]ACC EcoRII 96 CCAGG ^CC[AT]GG EcoRV 49 GATATC GAT^ATC HgaI 37 GCGTC GACGC(5/10) Hin1I 13 GGCGCC G[GA]^CG[CT]C HinfI 571 GAATC G^A[ACGT]TC KasI 13 GGCGCC G^GCGCC Ksp632I 455 GAAGAG CTCTTC(1/4) MvaI 96 CCAGG CC^[AT]GG MvnI 36 CGCG CG^CG NaeI 210 GCCGGC GCC^GGC NarI 13 GGCGCC GG^CGCC NciI 252 CCGGG CC^[GC]GG NgoMIV 210 GCCGGC G^CCGGC PflMI 475 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 59 GGGACCT [GA]G^G[AT]CC[CT] RsaI 411 GTAC GT^AC SapI 455 GAAGAGC GCTCTTC(1/4) Sau96I 60 GGACC G^G[ACGT]CC SexAI 95 ACCAGGT A^CC[AT]GGT SfoI 13 GGCGCC GGC^GCC SspBI 410 TGTACA T^GTACA TfiI 571 GAATC G^A[AT]TC Tsp45I 490 GTCAC ^GT[GC]AC TspRI 168 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 475 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 35 CCGC CCGC(-3/-1) 10 GCGG AluI 86 AGCT AG^CT 270 AGCT BanII 23 GGGCTC G[GA]GC[CT]^C 174 GAGCCC BbsI 256 GAAGAC GAAGAC(2/6) 123 GTCTTC BpuAI 256 GAAGAC GAAGAC(2/6) 123 GTCTTC BspHI 134 TCATGA T^CATGA 339 TCATGA BssKI 96 CCAGG ^CC[ACGT]GG 252 CCGGG CfoI 14 GCGC GCG^C 81 GCGC DpnII 117 GATC ^GATC 153 GATC DraI 102 TTTAAA TTT^AAA 289 TTTAAA FauI 34 CCCGC CCCGC(4/6) 10 GCGGG HaeII 13 GGCGCC [GA]GCGC^[CT] 80 AGCGCC HaeIII 213 GGCC GG^CC 421 GGCC HhaI 14 GCGC GCG^C 81 GCGC HinP1I 14 GCGC G^CGC 81 GCGC HphI 274 GGTGA GGTGA(8/7) 491 TCACC MaeII 446 ACGT A^CGT 567 ACGT MaeIII 490 GTCAC ^GT[ACGT]AC 558 GTTAC MboI 117 GATC ^GATC 153 GATC MseI 103 TTAA T^TAA 290 TTAA NdeI 476 CATATG CA^TATG 551 CATATG NdeII 117 GATC ^GATC 153 GATC RcaI 134 TCATGA T^CATGA 339 TCATGA Sau3AI 117 GATC ^GATC 153 GATC ScrFI 96 CCAGG CC^[ACGT]GG 252 CCGGG Tru9I 103 TTAA T^TAA 290 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AcsI ApoI BmyI BsiYI BslI BsmAI Bsp1286I Cac8I HapII HpaII MboII MnlI MslI MspI MwoI NlaIII NlaIV Tsp509I