Restriction map for pFN21AE6270
 (Circular) (Six-base) MAPSORT of: pFN21AE6270.seq  Check: 5604  from: 1  to: 5281



pFN21AE6270  5281 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:50  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3091     278 
   Size:         53      83     186     936    1555    2468

  Fragments arranged by size: 

                2468    1555     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2624    1937 
   Size:        687    4594
 
Acc65I G'GTAC_C

Cuts at:   2602    2602 
   Size:       5281
 
AclI AA'CG_TT

Cuts at:   1563    3408    3781    1563 
   Size:       1845     373    3063

  Fragments arranged by size: 

                3063    1845     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2090    3344    4728    4996    1632 
   Size:        458    1254    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1254     458     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5084
 
AflIII A'CryG_T

Cuts at:   1203    4180    5157    1203 
   Size:       2977     977    1327

  Fragments arranged by size: 

                2977    1327     977
 
AgeI A'CCGG_T

Cuts at:   4112    4112 
   Size:       5281
 
AhdI GACnn_n'nnGTC

Cuts at:   4010    4010 
   Size:       5281
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5281
 
AlwNI CAG_nnn'CTG

Cuts at:   3024    4596    3024 
   Size:       1572    3709
 
ApaLI G'TGCA_C

Cuts at:   3338    4494    3338 
   Size:       1156    4125
 
ApoI r'AATT_y

Cuts at:   1493    2251    2369    2522    2590    2816    2968    5077    1493 
   Size:        758     118     153      68     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     758     226     153     152     118      68
            
 
AseI AT'TA_AT

Cuts at:    160    1032    3835     160 
   Size:        872    2803    1606

  Fragments arranged by size: 

                2803    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5281
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2606    1870 
   Size:         73     663    4545

  Fragments arranged by size: 

                4545     663      73
 
BamHI G'GATC_C

Cuts at:   2611    2978    2611 
   Size:        367    4914
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2018    2602    4057     618 
   Size:        325     217     627     231     584    1455    1842

  Fragments arranged by size: 

                1842    1455     627     584     325     231     217
 
BanII G_rGCy'C

Cuts at:   1391    2032    2183    2600    1391 
   Size:        641     151     417    4072

  Fragments arranged by size: 

                4072     641     417     151
 
BbeI G_GCGC'C

Cuts at:   2022    2022 
   Size:       5281
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2121    2268     928 
   Size:        971     222     147    3941

  Fragments arranged by size: 

                3941     971     222     147
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3472    3506    1903 
   Size:         34    1535      34    3678

  Fragments arranged by size: 

                3678    1535      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3173    4383    3173 
   Size:       1210    4071
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5281
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2027    3892     136 
   Size:        107     122      71     631     605     355    1865    1525

  Fragments arranged by size: 

                1865    1525     631     605     355     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5276    5276 
   Size:       5281
 
BlpI GC'TnA_GC

Cuts at:   2698    2698 
   Size:       5281
 
Bme1580I G_kGCm'C

Cuts at:   1790    3342    4498    1790 
   Size:       1552    1156    2573

  Fragments arranged by size: 

                2573    1552    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2452    1318 
   Size:       1134    4147
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3012    3970     442 
   Size:        416      53      83     399      24    1595     958    1753

  Fragments arranged by size: 

                1753    1595     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5281
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2252    2252 
   Size:       5281
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3941    1239 
   Size:       2702    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3406    4271    4569    3406 
   Size:        865     298    4118

  Fragments arranged by size: 

                4118     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2226    3944     882 
   Size:        617     727    1718    2219

  Fragments arranged by size: 

                2219    1718     727     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3072     493 
   Size:        711    1868    2702

  Fragments arranged by size: 

                2702    1868     711
 
BsaBI GATnn'nnATC

Cuts at:   5275    5275 
   Size:       5281
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2019    3088    3470    4105 
   Size:         53      83     186     936     486    1069     382     635

Cuts at:   4105     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1069     936     635     486     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3714    4112    4386    4533    4944    3714 
   Size:        398     274     147     411    4051

  Fragments arranged by size: 

                4051     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5056

  Fragments arranged by size: 

                5056     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2244    1186 
   Size:       1058    4223
 
BseYI C'CCAG_C

Cuts at:   4484    4980    4484 
   Size:        496    4785
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2118    1163 
   Size:        573     382    4326

  Fragments arranged by size: 

                4326     573     382
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2731    3492    4520    1540 
   Size:        275     186     730     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     730     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2166    3342    3427    4498    2166 
   Size:       1176      85    1071    2949

  Fragments arranged by size: 

                2949    1176    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2792    2885    2792 
   Size:         93    5188
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2032    2166    2183    2600    3342    3427    4498 
   Size:        399     242     134      17     417     742      85    1071

Cuts at:   4498    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     417     399     242     134      85
                  17
 
BspEI T'CCGG_A

Cuts at:   4944    4944 
   Size:       5281
 
BspHI T'CATG_A

Cuts at:   2139    2344    3170    4900    2139 
   Size:        205     826    1730    2520

  Fragments arranged by size: 

                2520    1730     826     205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2636     844 
   Size:       1792    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3168    1394 
   Size:        429    1345    3507

  Fragments arranged by size: 

                3507    1345     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3776    3950      65 
   Size:       1183    2528     174    1396

  Fragments arranged by size: 

                2528    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2215    3925    4112    1700 
   Size:        110     405    1710     187    2869

  Fragments arranged by size: 

                2869    1710     405     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2415      96 
   Size:       2319    2962
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5281
 
BssSI C'ACGA_G

Cuts at:   3341    4353    3341 
   Size:       1012    4269
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5281
 
BstBI TT'CG_AA

Cuts at:   4085    4085 
   Size:       5281
 
BstEII G'GTnAC_C

Cuts at:   1184    2494    1184 
   Size:       1310    3971
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2191    2191 
   Size:       5281
 
BstYI r'GATC_y

Cuts at:   1972    2611    2978    3363    3380    4832    4918    4930    5276 
   Size:        639     367     385      17    1452      86      12     346

Cuts at:   5276    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     639     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2022     513 
   Size:        552     939      18    3772

  Fragments arranged by size: 

                3772     939     552      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4542

  Fragments arranged by size: 

                4542     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2179    2797    3591    3611    2179 
   Size:        618     794      20    3849

  Fragments arranged by size: 

                3849     794     618      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5281
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5246
 
DraI TTT'AAA

Cuts at:   2109    2296    2585    2109 
   Size:        187     289    4805

  Fragments arranged by size: 

                4805     289     187
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4288     809 
   Size:        507    2972    1802

  Fragments arranged by size: 

                2972    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2728    3617    5246 
   Size:         54    1425      50     275      18     898     889    1629

Cuts at:   5246       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     898     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2728    1537 
   Size:        275     916    4090

  Fragments arranged by size: 

                4090     916     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2455    3211    1353 
   Size:       1102     756    3423

  Fragments arranged by size: 

                3423    1102     756
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3868    4242    4388     387 
   Size:       1064     138    2279     374     146    1280

  Fragments arranged by size: 

                2279    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2090    3344    3941    4728    4996    1239 
   Size:        393     458    1254     597     787     268    1524

  Fragments arranged by size: 

                1524    1254     787     597     458     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2065    2065 
   Size:       5281
 
EcoRI G'AATT_C

Cuts at:   2590    2968    2590 
   Size:        378    4903
 
EcoRV GAT'ATC

Cuts at:   2056    2056 
   Size:       5281
 
FspI TGC'GCA

Cuts at:   3787    3787 
   Size:       5281
 
HaeII r_GCGC'y

Cuts at:   1678    2022    2089    4428    1678 
   Size:        344      67    2339    2531

  Fragments arranged by size: 

                2531    2339     344      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3922    3954    3996    4028    1373 
   Size:         32     135      32    2350      32      42      32    2626

  Fragments arranged by size: 

                2626    2350     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2625    2871     669 
   Size:        988     281     687     246    3079

  Fragments arranged by size: 

                3079     988     687     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5281
 
HpaI GTT'AAC

Cuts at:   2871    2871 
   Size:       5281
 
KasI G'GCGC_C

Cuts at:   2018    2018 
   Size:       5281
 
KpnI G_GTAC'C

Cuts at:   2606    2606 
   Size:       5281
 
MfeI C'AATT_G

Cuts at:   2880    2880 
   Size:       5281
 
MluI A'CGCG_T

Cuts at:   5157    5157 
   Size:       5281
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4395    4579    1305 
   Size:       3090     184    2007

  Fragments arranged by size: 

                3090    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3459

  Fragments arranged by size: 

                3459    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2123    2189    2504    3239    3598    3757     518 
   Size:        648     957      66     315     735     359     159    2042

  Fragments arranged by size: 

                2042     957     735     648     359     315     159      66
            
 
MspA1I CmG'CkG

Cuts at:   1606    2697    3374    4522    4767    1606 
   Size:       1091     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1091     677     245
 
NaeI GCC'GGC

Cuts at:   2217    2217 
   Size:       5281
 
NarI GG'CG_CC

Cuts at:   2019    2019 
   Size:       5281
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3790

  Fragments arranged by size: 

                3790     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2482    2557     387 
   Size:       2095      75    3111

  Fragments arranged by size: 

                3111    2095      75
 
NgoMIV G'CCGG_C

Cuts at:   2215    2215 
   Size:       5281
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5281
 
NotI GC'GGCC_GC

Cuts at:   2728    2728 
   Size:       5281
 
NspI r_CATG'y

Cuts at:   1132    1229    2639    4184    1132 
   Size:         97    1410    1545    2229

  Fragments arranged by size: 

                2229    1545    1410      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5281
 
PciI A'CATG_T

Cuts at:   4180    4180 
   Size:       5281
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2486    1108 
   Size:       1378    3903
 
PfoI T'CCnGG_A

Cuts at:   2256    2256 
   Size:       5281
 
PmeI GTTT'AAAC

Cuts at:   2585    2585 
   Size:       5281
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3350    3382    4863    4895    3350 
   Size:         32    1481      32    3736

  Fragments arranged by size: 

                3736    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2065    2065 
   Size:       5281
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5281
 
PsiI TTA'TAA

Cuts at:   2851    2851 
   Size:       5281
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2325    2357    2325 
   Size:         32    5249
 
PstI C_TGCA'G

Cuts at:    830    2633     830 
   Size:       1803    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5281
 
SalI G'TCGA_C

Cuts at:   1936    2623    1936 
   Size:        687    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2455    2455 
   Size:       5281
 
SbfI CC_TGCA'GG

Cuts at:   2633    2633 
   Size:       5281
 
ScaI AGT'ACT

Cuts at:   3529    5241    3529 
   Size:       1712    3569
 
SexAI A'CCwGG_T

Cuts at:   2100    2100 
   Size:       5281
 
SfcI C'TryA_G

Cuts at:    826    1045    2629    3764    4445    4636     826 
   Size:        219    1584    1135     681     191    1471

  Fragments arranged by size: 

                1584    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2020    2020 
   Size:       5281
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5281
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5281
 
SmaI CCC'GGG

Cuts at:   1872    2608    1872 
   Size:        736    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3385    4286    4548     820 
   Size:        197     926    1442     901     262    1553

  Fragments arranged by size: 

                1553    1442     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3072     493 
   Size:       2579    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5281
 
SphI G_CATG'C

Cuts at:   1132    2639    1132 
   Size:       1507    3774
 
SspI AAT'ATT

Cuts at:      5      52    3205       5 
   Size:         47    3153    2081

  Fragments arranged by size: 

                3153    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5281
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2720     513 
   Size:        552     939     716    3074

  Fragments arranged by size: 

                3074     939     716     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2540    3308    3325    3478    3663    4108     325 
   Size:       1255     960     768      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     960     768     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2415    3527    5239 
   Size:        274      80      33      51    1443     438    1112    1712

Cuts at:   5239      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1112     438     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5241    5273    5241 
   Size:         32    5249
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5281
 
XbaI T'CTAG_A

Cuts at:   2617    2617 
   Size:       5281
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5281
 
XmaI C'CCGG_G

Cuts at:   1870    2606    1870 
   Size:        736    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3410    3410 
   Size:       5281
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3089     276 
   Size:         53      83     186     936    1555    2468

  Fragments arranged by size: 

                2468    1555     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PsrI     PstI     PvuI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI     FalI     FseI    FspAI     NruI     NsiI     PacI     PmlI
   PspOMI    PspXI    PvuII    RsrII     SacI    SacII    SanDI     SfiI
     SrfI     SwaI     XcmI
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