################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccII AccIII AgeI AhdI AscI AseI AsnI AspEI AviII AvrII BbrPI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstUI BstZ17I CpoI EagI Eam1105I EclXI Eco52I FseI FspI HindIII KspI MamI MluI MroI MvnI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 4696 GACGTC GACGT^C Acc65I 1678 GGTACC G^GTACC AccI 2619 GTCTAC GT^[AC][GT]AC AclI 3628 AACGTT AA^CGTT AflII 5860 CTTAAG C^TTAAG AflIII 2440 ACGTGT A^C[GA][CT]GT Alw44I 5866 GTGCAC G^TGCAC Aor51HI 3554 AGCGCT AGC^GCT ApaI 16 GGGCCC GGGCC^C ApaLI 5866 GTGCAC G^TGCAC Asp718I 1678 GGTACC G^GTACC BamHI 5010 GGATCC G^GATCC BbeI 4285 GGCGCC GGCGC^C BcgI 5083 GCAGGCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5655 GGATAC GTATCC(6/5) BclI 6336 TGATCA T^GATCA BfrI 5860 CTTAAG C^TTAAG BglII 5978 AGATCT A^GATCT BsaAI 2622 TACGTG [CT]AC^GT[GA] BsmBI 1537 GAGACG CGTCTC(1/5) Bsp1407I 3966 TGTACA T^GTACA BspDI 154 ATCGAT AT^CGAT BsrFI 4344 GCCGGC [GA]^CCGG[CT] BsrGI 3966 TGTACA T^GTACA Cfr10I 4344 GCCGGC [GA]^CCGG[CT] ClaI 154 ATCGAT AT^CGAT Eco47III 3554 AGCGCT AGC^GCT EcoNI 6001 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3646 GATATC GAT^ATC EcoT22I 2102 ATGCAT ATGCA^T FbaI 6336 TGATCA T^GATCA HpaI 4105 GTTAAC GTT^AAC KasI 4285 GGCGCC G^GCGCC KpnI 1678 GGTACC GGTAC^C NaeI 4344 GCCGGC GCC^GGC NarI 4285 GGCGCC GG^CGCC NcoI 710 CCATGG C^CATGG NgoMIV 4344 GCCGGC G^CCGGC NsiI 2102 ATGCAT ATGCA^T Psp1406I 3628 AACGTT AA^CGTT ScaI 2674 AGTACT AGT^ACT SexAI 4816 ACCAGGT A^CC[AT]GGT SfiI 17 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4285 GGCGCC GGC^GCC SspBI 3966 TGTACA T^GTACA SspI 2486 AATATT AAT^ATT XbaI 3720 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 4285 GGCGCC G[GA]^CG[CT]C 4696 GACGTC BbvCI 4943 CCTCAGC CCTCAGC(-5/-2) 5170 GCTGAGG BlpI 1711 GCTGAGC GC^T[ACGT]AGC 4570 GCTGAGC Bpu1102I 1711 GCTGAGC GC^T[ACGT]AGC 4570 GCTGAGC BsaHI 4285 GGCGCC G[GA]^CG[CT]C 4696 GACGTC BssSI 2291 CTCGTG CACGAG(-5/-1) 3122 CTCGTG BstEII 733 GGTGACC G^GT[ACGT]ACC 2428 GGTGACC BstPI 733 GGTGACC G^GT[ACGT]ACC 2428 GGTGACC BstXI 1221 CCAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4007 CCAGACTCCTGG CelII 1711 GCTGAGC GC^T[ACGT]AGC 4570 GCTGAGC CfoI 3555 GCGC GCG^C 4286 GCGC DraIII 3227 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 6196 CACTGGGTG DrdI 2595 GACACAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2610 GACTTCAGTGTC EcoO65I 733 GGTGACC G^GT[ACGT]ACC 2428 GGTGACC HaeII 3554 AGCGCT [GA]GCGC^[CT] 4285 GGCGCC HhaI 3555 GCGC GCG^C 4286 GCGC Hin1I 4285 GGCGCC G[GA]^CG[CT]C 4696 GACGTC HinP1I 3555 GCGC G^CGC 4286 GCGC HincII 4105 GTTAAC GT[CT]^[GA]AC 4663 GTTGAC HindII 4105 GTTAAC GT[CT]^[GA]AC 4663 GTTGAC MfeI 334 CAATTG C^AATTG 2939 CAATTG MunI 334 CAATTG C^AATTG 2939 CAATTG PpuMI 1299 GGGACCT [GA]G^G[AT]CC[CT] 5098 GGGACCC PshAI 3570 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3787 GACGGTGGTC SacI 5351 GAGCTC GAGCT^C 5905 GAGCTC SapI 3949 GAAGAGC GCTCTTC(1/4) 5348 GAAGAGC SmaI 462 CCCGGG CCC^GGG 3989 CCCGGG SphI 1901 GCATGC GCATG^C 3093 GCATGC TaqI 155 TCGA T^CGA 5282 TCGA TthHB8I 155 TCGA T^CGA 5282 TCGA XmaCI 462 CCCGGG C^CCGGG 3989 CCCGGG XmaI 462 CCCGGG C^CCGGG 3989 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XhoII XmnI