(Circular) (Six-base) MAPSORT of: pFN21AE3714.seq Check: 9095 from: 1 to: 11215 pFN21AE3714 11215 bp With 182 enzymes: SgfI * March 28, 2012 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5093 5093 Size: 11215 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6705 9025 278 Size: 53 83 186 936 5169 2320 2468 Fragments arranged by size: 5169 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4625 8558 1937 Size: 2688 3933 4594 Fragments arranged by size: 4594 3933 2688 Acc65I G'GTAC_C Cuts at: 3683 8536 3683 Size: 4853 6362 AclI AA'CG_TT Cuts at: 1563 5634 9342 9715 1563 Size: 4071 3708 373 3063 Fragments arranged by size: 4071 3708 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2803 3312 4291 4602 5291 5732 6867 7650 Size: 1171 509 979 311 689 441 1135 783 Cuts at: 7650 8016 9278 10662 10930 1632 Size: 366 1262 1384 268 1917 Fragments arranged by size: 1917 1384 1262 1171 1135 979 783 689 509 441 366 311 268 AfeI AGC'GCT Cuts at: 5561 5561 Size: 11215 AflII C'TTAA_G Cuts at: 820 1017 7865 820 Size: 197 6848 4170 Fragments arranged by size: 6848 4170 197 AflIII A'CryG_T Cuts at: 1203 4445 10114 11091 1203 Size: 3242 5669 977 1327 Fragments arranged by size: 5669 3242 1327 977 AgeI A'CCGG_T Cuts at: 10046 10046 Size: 11215 AhdI GACnn_n'nnGTC Cuts at: 9944 9944 Size: 11215 AleI CACnn'nnGTG Cuts at: 1166 7115 1166 Size: 5949 5266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4028 7521 7835 1184 Size: 2844 3493 314 4564 Fragments arranged by size: 4564 3493 2844 314 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7773 7805 7773 Size: 32 11183 AlwNI CAG_nnn'CTG Cuts at: 2090 3776 4031 4355 5428 5626 5852 8008 8174 Size: 1686 255 324 1073 198 226 2156 166 Cuts at: 8174 8489 8958 10530 2090 Size: 315 469 1572 2775 Fragments arranged by size: 2775 2156 1686 1572 1073 469 324 315 255 226 198 166 ApaI G_GGCC'C Cuts at: 2025 2025 Size: 11215 ApaLI G'TGCA_C Cuts at: 7871 9272 10428 7871 Size: 1401 1156 8658 Fragments arranged by size: 8658 1401 1156 ApoI r'AATT_y Cuts at: 1493 2189 3098 3396 3437 4506 4750 5377 5770 Size: 696 909 298 41 1069 244 627 393 Cuts at: 5770 6447 6513 6693 7194 7440 7613 7685 8111 Size: 677 66 180 501 246 173 72 426 Cuts at: 8111 8524 8750 8902 11011 1493 Size: 413 226 152 2109 1697 Fragments arranged by size: 2109 1697 1069 909 696 677 627 501 426 413 393 298 246 244 226 180 173 152 72 66 41 AseI AT'TA_AT Cuts at: 160 1032 9769 160 Size: 872 8737 1606 Fragments arranged by size: 8737 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11215 AvaI C'yCGr_G Cuts at: 1870 1943 2467 3159 3368 5994 7083 8540 1870 Size: 73 524 692 209 2626 1089 1457 4545 Fragments arranged by size: 4545 2626 1457 1089 692 524 209 73 BamHI G'GATC_C Cuts at: 7015 8545 8912 7015 Size: 1530 367 9318 Fragments arranged by size: 9318 1530 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3683 4718 5230 5512 6290 Size: 325 217 627 1896 1035 512 282 778 Cuts at: 6290 7031 8536 9991 618 Size: 741 1505 1455 1842 Fragments arranged by size: 1896 1842 1505 1455 1035 778 741 627 512 325 282 217 BanII G_rGCy'C Cuts at: 1391 2025 3723 4895 5961 6270 6729 7360 7914 Size: 634 1698 1172 1066 309 459 631 554 Cuts at: 7914 8534 1391 Size: 620 4072 Fragments arranged by size: 4072 1698 1172 1066 634 631 620 554 459 309 BbeI G_GCGC'C Cuts at: 6294 6294 Size: 11215 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2916 3318 4215 5619 6629 6962 7716 Size: 971 1017 402 897 1404 1010 333 754 Cuts at: 7716 7831 928 Size: 115 4312 Fragments arranged by size: 4312 1404 1017 1010 971 897 754 402 333 115 BbvCI CC'TCA_GC Cuts at: 6949 7176 6949 Size: 227 10988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7077 7111 9406 9440 1903 Size: 34 5140 34 2295 34 3678 Fragments arranged by size: 5140 3678 2295 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7654 9107 10317 7654 Size: 1453 1210 8552 Fragments arranged by size: 8552 1453 1210 BclI T'GATC_A Cuts at: 1548 8341 1548 Size: 6793 4422 BfrBI ATG'CAT Cuts at: 4109 4109 Size: 11215 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2029 5949 6420 Size: 107 122 71 631 605 357 3920 471 Cuts at: 6420 9826 136 Size: 3406 1525 Fragments arranged by size: 3920 3406 1525 631 605 471 357 122 107 71 BglII A'GATC_T Cuts at: 7983 11210 7983 Size: 3227 7988 BlpI GC'TnA_GC Cuts at: 3717 6576 8632 3717 Size: 2859 2056 6300 Fragments arranged by size: 6300 2859 2056 Bme1580I G_kGCm'C Cuts at: 1790 2025 2474 5233 5517 7034 7875 9276 10432 Size: 235 449 2759 284 1517 841 1401 1156 Cuts at: 10432 1790 Size: 2573 Fragments arranged by size: 2759 2573 1517 1401 1156 841 449 284 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11215 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4065 4890 5229 Size: 416 53 83 399 24 2648 825 339 Cuts at: 5229 8212 8946 9904 442 Size: 2983 734 958 1753 Fragments arranged by size: 2983 2648 1753 958 825 734 416 399 339 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11215 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3975 4015 5142 3975 Size: 40 1127 10048 Fragments arranged by size: 10048 1127 40 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2510 2622 3005 3970 5346 6041 6114 6359 Size: 1271 112 383 965 1376 695 73 245 Cuts at: 6359 7344 9875 1239 Size: 985 2531 2579 Fragments arranged by size: 2579 2531 1376 1271 985 965 695 383 245 112 73 Bpu10I CC'TnA_GC Cuts at: 5563 6565 6949 7176 5563 Size: 1002 384 227 9602 Fragments arranged by size: 9602 1002 384 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 4485 6131 8176 9340 10205 10503 4258 Size: 227 1646 2045 1164 865 298 4970 Fragments arranged by size: 4970 2045 1646 1164 865 298 227 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3162 4471 5587 5804 8486 9878 882 Size: 617 1663 1309 1116 217 2682 1392 2219 Fragments arranged by size: 2682 2219 1663 1392 1309 1116 617 217 BsaAI yAC'GTr Cuts at: 493 1204 4629 9006 493 Size: 711 3425 4377 2702 Fragments arranged by size: 4377 3425 2702 711 BsaBI GATnn'nnATC Cuts at: 11209 11209 Size: 11215 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6291 6702 9022 9404 Size: 53 83 186 936 4758 411 2320 382 Cuts at: 9404 10039 275 Size: 635 1451 Fragments arranged by size: 4758 2320 1451 936 635 411 382 186 83 53 BsaWI w'CCGG_w Cuts at: 9648 10046 10320 10467 10878 9648 Size: 398 274 147 411 9985 Fragments arranged by size: 9985 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2508 2538 3854 3884 6000 Size: 30 165 30 1105 30 1316 30 2116 Cuts at: 6000 6030 6826 6856 7773 7803 8100 8130 1178 Size: 30 796 30 917 30 297 30 4263 Fragments arranged by size: 4263 2116 1316 1105 917 796 297 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2482 2978 3256 3951 4055 5129 6473 6937 Size: 1296 496 278 695 104 1074 1344 464 Cuts at: 6937 7145 7781 8108 8111 1186 Size: 208 636 327 3 4290 Fragments arranged by size: 4290 1344 1296 1074 695 636 496 464 327 278 208 104 3 BseYI C'CCAG_C Cuts at: 2888 3049 3176 4034 4805 5059 5433 5603 6494 Size: 161 127 858 771 254 374 170 891 Cuts at: 6494 8386 10418 10914 2888 Size: 1892 2032 496 3189 Fragments arranged by size: 3189 2032 1892 891 858 771 496 374 254 170 161 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2829 5189 5436 5475 7517 7527 7757 Size: 573 1093 2360 247 39 2042 10 230 Cuts at: 7757 1163 Size: 4621 Fragments arranged by size: 4621 2360 2042 1093 573 247 230 39 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8665 9426 10454 1540 Size: 275 186 6664 761 1028 2301 Fragments arranged by size: 6664 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2324 7360 7875 7914 9276 9361 10432 2324 Size: 5036 515 39 1362 85 1071 3107 Fragments arranged by size: 5036 3107 1362 1071 515 85 39 BsmI GAATG_Cn' Cuts at: 2710 3658 7952 8726 8819 2710 Size: 948 4294 774 93 5106 Fragments arranged by size: 5106 4294 948 774 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3536 3536 Size: 11215 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2324 2474 3723 4895 5233 5517 Size: 399 235 299 150 1249 1172 338 284 Cuts at: 5517 5961 6270 6729 7034 7360 7875 7914 8534 Size: 444 309 459 305 326 515 39 620 Cuts at: 8534 9276 9361 10432 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1249 1172 1071 742 620 515 459 444 399 338 326 309 305 299 284 235 150 85 39 BspEI T'CCGG_A Cuts at: 10878 10878 Size: 11215 BspHI T'CATG_A Cuts at: 2761 6697 7444 9104 10834 2761 Size: 3936 747 1660 1730 3142 Fragments arranged by size: 3936 3142 1730 1660 747 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3054 5093 6194 8570 844 Size: 2210 2039 1101 2376 3489 Fragments arranged by size: 3489 2376 2210 2039 1101 BsrBI CCG'CTC Cuts at: 1394 1823 9102 1394 Size: 429 7279 3507 Fragments arranged by size: 7279 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2054 3109 4419 5040 7881 9710 9884 Size: 1183 806 1055 1310 621 2841 1829 174 Cuts at: 9884 65 Size: 1396 Fragments arranged by size: 2841 1829 1396 1310 1183 1055 806 621 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6349 9859 10046 1700 Size: 110 4539 3510 187 2869 Fragments arranged by size: 4539 3510 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5971 96 Size: 5875 5340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11215 BssSI C'ACGA_G Cuts at: 4296 5127 9275 10287 4296 Size: 831 4148 1012 5224 Fragments arranged by size: 5224 4148 1012 831 BstAPI GCAn_nnn'nTGC Cuts at: 794 4409 6470 6487 794 Size: 3615 2061 17 5522 Fragments arranged by size: 5522 3615 2061 17 BstBI TT'CG_AA Cuts at: 10019 10019 Size: 11215 BstEII G'GTnAC_C Cuts at: 1184 2738 4433 1184 Size: 1554 1695 7966 Fragments arranged by size: 7966 1695 1554 BstXI CCAn_nnnn'nTGG Cuts at: 3233 6019 3233 Size: 2786 8429 BstYI r'GATC_y Cuts at: 1972 6214 7015 7377 7983 8545 8912 9297 9314 Size: 4242 801 362 606 562 367 385 17 Cuts at: 9314 10766 10852 10864 11210 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 4242 1977 1452 801 606 562 385 367 362 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3963 5088 6456 3963 Size: 1125 1368 8722 Fragments arranged by size: 8722 1368 1125 BtgI C'CryG_G Cuts at: 513 1065 2715 2871 5517 5586 6030 7761 513 Size: 552 1650 156 2646 69 444 1731 3967 Fragments arranged by size: 3967 2646 1731 1650 552 444 156 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3096 487 Size: 683 56 1870 8606 Fragments arranged by size: 8606 1870 683 56 BtsI GCAGTG_nn' Cuts at: 3192 3360 4410 6159 6559 7122 7168 8731 9525 Size: 168 1050 1749 400 563 46 1563 794 Cuts at: 9525 9545 3192 Size: 20 4862 Fragments arranged by size: 4862 1749 1563 1050 794 563 400 168 46 20 ClaI AT'CG_AT Cuts at: 1554 2160 1554 Size: 606 10609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11180 DraI TTT'AAA Cuts at: 4873 6172 8314 8350 8519 4873 Size: 1299 2142 36 169 7569 Fragments arranged by size: 7569 2142 1299 169 36 DraIII CAC_nnn'GTG Cuts at: 5237 8206 5237 Size: 2969 8246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4606 4621 10222 809 Size: 507 3290 15 5601 1802 Fragments arranged by size: 5601 3290 1802 507 15 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2874 4786 5750 Size: 54 1425 50 275 18 1044 1912 964 Cuts at: 5750 6351 7849 8662 9551 11180 8 Size: 601 1498 813 889 1629 43 Fragments arranged by size: 1912 1629 1498 1425 1044 964 889 813 601 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8662 1537 Size: 275 6850 4090 Fragments arranged by size: 6850 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3084 5310 5367 5658 5949 6811 7305 7348 Size: 1731 2226 57 291 291 862 494 43 Cuts at: 7348 7671 8155 9145 1353 Size: 323 484 990 3423 Fragments arranged by size: 3423 2226 1731 990 862 494 484 323 291 291 57 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9802 10176 10322 387 Size: 1064 138 8213 374 146 1280 Fragments arranged by size: 8213 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 7358 7912 7358 Size: 554 10661 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 2622 2803 3005 3312 3970 4291 Size: 393 878 112 181 202 307 658 321 Cuts at: 4291 4602 5291 5346 5732 6041 6114 6359 6867 Size: 311 689 55 386 309 73 245 508 Cuts at: 6867 7344 7650 8016 9278 9875 10662 10930 1239 Size: 477 306 366 1262 597 787 268 1524 Fragments arranged by size: 1524 1262 878 787 689 658 597 508 477 393 386 366 321 311 309 307 306 268 245 202 181 112 73 55 EcoNI CCTnn'n_nnAGG Cuts at: 8010 8010 Size: 11215 EcoO109I rG'GnC_Cy Cuts at: 2021 2022 2031 3305 7104 8486 2021 Size: 1 9 1274 3799 1382 4750 Fragments arranged by size: 4750 3799 1382 1274 9 1 EcoRI G'AATT_C Cuts at: 2189 3098 3396 4750 6513 7685 8524 8902 2189 Size: 909 298 1354 1763 1172 839 378 4502 Fragments arranged by size: 4502 1763 1354 1172 909 839 378 298 EcoRV GAT'ATC Cuts at: 5653 5653 Size: 11215 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3704 3768 5237 6006 3704 Size: 64 1469 769 8913 Fragments arranged by size: 8913 1469 769 64 FspI TGC'GCA Cuts at: 9721 9721 Size: 11215 HaeII r_GCGC'y Cuts at: 1678 5563 6294 10362 1678 Size: 3885 731 4068 2531 Fragments arranged by size: 4068 3885 2531 731 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2309 2341 3308 3339 3340 Size: 32 135 32 737 32 967 31 1 Cuts at: 3340 3371 3854 3886 3981 4013 4241 4273 4637 Size: 31 483 32 95 32 228 32 364 Cuts at: 4637 4669 4882 4914 5012 5044 5306 5338 5707 Size: 32 213 32 98 32 262 32 369 Cuts at: 5707 5739 5900 5932 6059 6091 7466 7498 9856 Size: 32 161 32 127 32 1375 32 2358 Cuts at: 9856 9888 9930 9962 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2358 1375 967 737 483 369 364 262 228 213 161 135 127 98 95 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 6112 6670 8559 8805 669 Size: 988 281 4174 558 1889 246 3079 Fragments arranged by size: 4174 3079 1889 988 558 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 11215 HpaI GTT'AAC Cuts at: 6112 8805 6112 Size: 2693 8522 KasI G'GCGC_C Cuts at: 6290 6290 Size: 11215 KpnI G_GTAC'C Cuts at: 3687 8540 3687 Size: 4853 6362 MfeI C'AATT_G Cuts at: 2339 4944 8814 2339 Size: 2605 3870 4740 Fragments arranged by size: 4740 3870 2605 MluI A'CGCG_T Cuts at: 11091 11091 Size: 11215 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 2357 2532 3761 5154 5488 10329 10513 Size: 900 152 175 1229 1393 334 4841 184 Cuts at: 10513 1305 Size: 2007 Fragments arranged by size: 4841 2007 1393 1229 900 334 184 175 152 MscI TGG'CCA Cuts at: 10 64 1489 1832 2876 4788 5752 7851 10 Size: 54 1425 343 1044 1912 964 2099 3374 Fragments arranged by size: 3374 2099 1912 1425 1044 964 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 2673 3315 3795 5103 7115 7817 Size: 648 1386 121 642 480 1308 2012 702 Cuts at: 7817 7857 9173 9532 9691 518 Size: 40 1316 359 159 2042 Fragments arranged by size: 2042 2012 1386 1316 1308 702 648 642 480 359 159 121 40 MspA1I CmG'CkG Cuts at: 1606 2038 2486 3551 6080 7628 7645 8631 9308 Size: 432 448 1065 2529 1548 17 986 677 Cuts at: 9308 10456 10701 1606 Size: 1148 245 2120 Fragments arranged by size: 2529 2120 1548 1148 1065 986 677 448 432 245 17 NaeI GCC'GGC Cuts at: 6351 6351 Size: 11215 NarI GG'CG_CC Cuts at: 6291 6291 Size: 11215 NcoI C'CATG_G Cuts at: 513 1065 2715 513 Size: 552 1650 9013 Fragments arranged by size: 9013 1650 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 11215 NgoMIV G'CCGG_C Cuts at: 6349 6349 Size: 11215 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11215 NotI GC'GGCC_GC Cuts at: 8662 8662 Size: 11215 NsiI A_TGCA'T Cuts at: 4111 4111 Size: 11215 NspI r_CATG'y Cuts at: 1132 1229 3065 3910 4784 5102 8248 8573 10118 Size: 97 1836 845 874 318 3146 325 1545 Cuts at: 10118 1132 Size: 2229 Fragments arranged by size: 3146 2229 1836 1545 874 845 325 318 97 PasI CC'CwG_GG Cuts at: 1348 7036 1348 Size: 5688 5527 PciI A'CATG_T Cuts at: 10114 10114 Size: 11215 PflMI CCAn_nnn'nTGG Cuts at: 1108 2533 4055 5344 6883 7163 1108 Size: 1425 1522 1289 1539 280 5160 Fragments arranged by size: 5160 1539 1522 1425 1289 280 PfoI T'CCnGG_A Cuts at: 3391 6018 3391 Size: 2627 8588 PmeI GTTT'AAAC Cuts at: 8519 8519 Size: 11215 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5105 5137 7773 7805 9284 9316 10797 10829 5105 Size: 32 2636 32 1479 32 1481 32 5491 Fragments arranged by size: 5491 2636 1481 1479 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3305 7104 3305 Size: 3799 7416 PshAI GACnn'nnGTC Cuts at: 1613 5579 5796 1613 Size: 3966 217 7032 Fragments arranged by size: 7032 3966 217 PsiI TTA'TAA Cuts at: 2445 5720 8785 2445 Size: 3275 3065 4875 Fragments arranged by size: 4875 3275 3065 PspOMI G'GGCC_C Cuts at: 2021 2021 Size: 11215 PstI C_TGCA'G Cuts at: 830 5268 5294 7014 8567 830 Size: 4438 26 1720 1553 3478 Fragments arranged by size: 4438 3478 1720 1553 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11215 PvuII CAG'CTG Cuts at: 2486 3551 7628 7645 2486 Size: 1065 4077 17 6056 Fragments arranged by size: 6056 4077 1065 17 SacI G_AGCT'C Cuts at: 7360 7914 7360 Size: 554 10661 SalI G'TCGA_C Cuts at: 1936 8557 1936 Size: 6621 4594 SanDI GG'GwC_CC Cuts at: 7104 7104 Size: 11215 SapI GCTCTTCn'nnn_ Cuts at: 5949 7348 5949 Size: 1399 9816 SbfI CC_TGCA'GG Cuts at: 8567 8567 Size: 11215 ScaI AGT'ACT Cuts at: 4681 9463 11175 4681 Size: 4782 1712 4721 Fragments arranged by size: 4782 4721 1712 SexAI A'CCwGG_T Cuts at: 6821 6821 Size: 11215 SfcI C'TryA_G Cuts at: 826 1045 4515 5264 5290 6039 6210 7010 8085 Size: 219 3470 749 26 749 171 800 1075 Cuts at: 8085 8563 9698 10379 10570 826 Size: 478 1135 681 191 1471 Fragments arranged by size: 3470 1471 1135 1075 800 749 749 681 478 219 191 171 26 SfiI GGCCn_nnn'nGGCC Cuts at: 2029 2029 Size: 11215 SfoI GGC'GCC Cuts at: 6292 6292 Size: 11215 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11215 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11215 SmaI CCC'GGG Cuts at: 1872 2469 5996 8542 1872 Size: 597 3527 2546 4545 Fragments arranged by size: 4545 3527 2546 597 SmlI C'TyrA_G Cuts at: 820 1017 1943 4273 4500 6146 7865 8155 9319 Size: 197 926 2330 227 1646 1719 290 1164 Cuts at: 9319 10220 10482 820 Size: 901 262 1553 Fragments arranged by size: 2330 1719 1646 1553 1164 926 901 290 262 227 197 SnaBI TAC'GTA Cuts at: 493 9006 493 Size: 8513 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11215 SphI G_CATG'C Cuts at: 1132 3910 5102 8573 1132 Size: 2778 1192 3471 3774 Fragments arranged by size: 3774 3471 2778 1192 SspI AAT'ATT Cuts at: 5 52 4493 9139 5 Size: 47 4441 4646 2081 Fragments arranged by size: 4646 4441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 11215 StyI C'CwwG_G Cuts at: 513 1065 2715 4287 4388 4438 4740 4958 6960 Size: 552 1650 1572 101 50 302 218 2002 Cuts at: 6960 8654 513 Size: 1694 3074 Fragments arranged by size: 3074 2002 1694 1650 1572 552 302 218 101 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2567 3107 3111 5073 5248 8194 9242 Size: 1255 987 540 4 1962 175 2946 1048 Cuts at: 9242 9259 9412 9597 10042 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2946 1962 1498 1255 1048 987 540 445 185 175 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2800 3671 4679 Size: 274 80 33 51 1443 823 871 1008 Cuts at: 4679 4777 5971 6243 6833 9461 11173 96 Size: 98 1194 272 590 2628 1712 138 Fragments arranged by size: 2628 1712 1443 1194 1008 871 823 590 274 272 138 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2508 2540 4361 4393 5244 5276 5445 5477 8100 Size: 32 1821 32 851 32 169 32 2623 Cuts at: 8100 8132 11175 11207 2508 Size: 32 3043 32 2516 Fragments arranged by size: 3043 2623 2516 1821 851 169 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3866 4255 5816 7123 1316 Size: 2550 389 1561 1307 5408 Fragments arranged by size: 5408 2550 1561 1307 389 XbaI T'CTAG_A Cuts at: 5725 8551 5725 Size: 2826 8389 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2133 4762 8219 2133 Size: 2629 3457 5129 Fragments arranged by size: 5129 3457 2629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11215 XmaI C'CCGG_G Cuts at: 1870 2467 5994 8540 1870 Size: 597 3527 2546 4545 Fragments arranged by size: 4545 3527 2546 597 XmnI GAAnn'nnTTC Cuts at: 3325 5986 6447 6513 7788 9344 3325 Size: 2661 461 66 1275 1556 5196 Fragments arranged by size: 5196 2661 1556 1275 461 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6703 9023 276 Size: 53 83 186 936 5169 2320 2468 Fragments arranged by size: 5169 2468 2320 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SrfI SwaI