(Linear) (Six-base) MAPSORT of: KIEE3714.seq Check: 8688 from: 1 to: 6510 KIEE3714.orf With 182 enzymes: SgfI * March 22, 2012 16:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3088 6510 Size: 3088 3422 AatII G_ACGT'C Cuts at: 0 4700 6510 Size: 4700 1810 AccI GT'mk_AC Cuts at: 0 2620 6510 Size: 2620 3890 Acc65I G'GTAC_C Cuts at: 0 1678 6510 Size: 1678 4832 AclI AA'CG_TT Cuts at: 0 3629 6510 Size: 3629 2881 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 798 1307 2286 2597 3286 3727 4862 5645 Size: 798 509 979 311 689 441 1135 783 Cuts at: 5645 6011 6510 Size: 366 499 Fragments arranged by size: 1135 979 798 783 689 509 499 441 366 311 AfeI AGC'GCT Cuts at: 0 3556 6510 Size: 3556 2954 AflII C'TTAA_G Cuts at: 0 5860 6510 Size: 5860 650 AflIII A'CryG_T Cuts at: 0 2440 6510 Size: 2440 4070 AleI CACnn'nnGTG Cuts at: 0 5110 6510 Size: 5110 1400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2023 5516 5830 6510 Size: 2023 3493 314 680 Fragments arranged by size: 3493 2023 680 314 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5768 5800 6510 Size: 5768 32 710 Fragments arranged by size: 5768 710 32 AlwNI CAG_nnn'CTG Cuts at: 0 85 1771 2026 2350 3423 3621 3847 6003 Size: 85 1686 255 324 1073 198 226 2156 Cuts at: 6003 6169 6484 6510 Size: 166 315 26 Fragments arranged by size: 2156 1686 1073 324 315 255 226 198 166 85 26 ApaI G_GGCC'C Cuts at: 0 20 6510 Size: 20 6490 ApaLI G'TGCA_C Cuts at: 0 5866 6510 Size: 5866 644 ApoI r'AATT_y Cuts at: 0 184 1093 1391 1432 2501 2745 3372 3765 Size: 184 909 298 41 1069 244 627 393 Cuts at: 3765 4442 4508 4688 5189 5435 5608 5680 6106 Size: 677 66 180 501 246 173 72 426 Cuts at: 6106 6510 Size: 404 Fragments arranged by size: 1069 909 677 627 501 426 404 393 298 246 244 184 180 173 72 66 41 AvaI C'yCGr_G Cuts at: 0 462 1154 1363 3989 5078 6510 Size: 462 692 209 2626 1089 1432 Fragments arranged by size: 2626 1432 1089 692 462 209 BamHI G'GATC_C Cuts at: 0 5010 6510 Size: 5010 1500 BanI G'GyrC_C Cuts at: 0 1678 2713 3225 3507 4285 5026 6510 Size: 1678 1035 512 282 778 741 1484 Fragments arranged by size: 1678 1484 1035 778 741 512 282 BanII G_rGCy'C Cuts at: 0 20 1718 2890 3956 4265 4724 5355 5909 Size: 20 1698 1172 1066 309 459 631 554 Cuts at: 5909 6510 Size: 601 Fragments arranged by size: 1698 1172 1066 631 601 554 459 309 20 BbeI G_GCGC'C Cuts at: 0 4289 6510 Size: 4289 2221 BbsI GAAGACnn'nnnn_ Cuts at: 0 911 1313 2210 3614 4624 4957 5711 5826 Size: 911 402 897 1404 1010 333 754 115 Cuts at: 5826 6510 Size: 684 Fragments arranged by size: 1404 1010 911 897 754 684 402 333 115 BbvCI CC'TCA_GC Cuts at: 0 4944 5171 6510 Size: 4944 227 1339 Fragments arranged by size: 4944 1339 227 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 5072 5106 6510 Size: 5072 34 1404 Fragments arranged by size: 5072 1404 34 BciVI GTATCCnnnnn_n' Cuts at: 0 5649 6510 Size: 5649 861 BclI T'GATC_A Cuts at: 0 6336 6510 Size: 6336 174 BfrBI ATG'CAT Cuts at: 0 2104 6510 Size: 2104 4406 BglI GCCn_nnn'nGGC Cuts at: 0 24 3944 4415 6510 Size: 24 3920 471 2095 Fragments arranged by size: 3920 2095 471 24 BglII A'GATC_T Cuts at: 0 5978 6510 Size: 5978 532 BlpI GC'TnA_GC Cuts at: 0 1712 4571 6510 Size: 1712 2859 1939 Fragments arranged by size: 2859 1939 1712 Bme1580I G_kGCm'C Cuts at: 0 20 469 3228 3512 5029 5870 6510 Size: 20 449 2759 284 1517 841 640 Fragments arranged by size: 2759 1517 841 640 449 284 20 BmrI ACTGGGnnnn_n' Cuts at: 0 2060 2885 3224 6207 6510 Size: 2060 825 339 2983 303 Fragments arranged by size: 2983 2060 825 339 303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1970 2010 3137 6510 Size: 1970 40 1127 3373 Fragments arranged by size: 3373 1970 1127 40 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 617 1000 1965 3341 4036 4109 4354 Size: 505 112 383 965 1376 695 73 245 Cuts at: 4354 5339 6510 Size: 985 1171 Fragments arranged by size: 1376 1171 985 965 695 505 383 245 112 73 Bpu10I CC'TnA_GC Cuts at: 0 3558 4560 4944 5171 6510 Size: 3558 1002 384 227 1339 Fragments arranged by size: 3558 1339 1002 384 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2253 2480 4126 6171 6510 Size: 2253 227 1646 2045 339 Fragments arranged by size: 2253 2045 1646 339 227 BsaI GGTCTCn'nnnn_ Cuts at: 0 1157 2466 3582 3799 6481 6510 Size: 1157 1309 1116 217 2682 29 Fragments arranged by size: 2682 1309 1157 1116 217 29 BsaAI yAC'GTr Cuts at: 0 2624 6510 Size: 2624 3886 BsaHI Gr'CG_yC Cuts at: 0 4286 4697 6510 Size: 4286 411 1813 Fragments arranged by size: 4286 1813 411 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 503 533 1849 1879 3995 4025 4821 4851 Size: 503 30 1316 30 2116 30 796 30 Cuts at: 4851 5768 5798 6095 6125 6510 Size: 917 30 297 30 385 Fragments arranged by size: 2116 1316 917 796 503 385 297 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 477 973 1251 1946 2050 3124 4468 4932 Size: 477 496 278 695 104 1074 1344 464 Cuts at: 4932 5140 5776 6103 6106 6510 Size: 208 636 327 3 404 Fragments arranged by size: 1344 1074 695 636 496 477 464 404 327 278 208 104 3 BseYI C'CCAG_C Cuts at: 0 883 1044 1171 2029 2800 3054 3428 3598 Size: 883 161 127 858 771 254 374 170 Cuts at: 3598 4489 6381 6510 Size: 891 1892 129 Fragments arranged by size: 1892 891 883 858 771 374 254 170 161 129 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 824 3184 3431 3470 5512 5522 5752 6510 Size: 824 2360 247 39 2042 10 230 758 Fragments arranged by size: 2360 2042 824 758 247 230 39 10 BsiHKAI G_wGCw'C Cuts at: 0 319 5355 5870 5909 6510 Size: 319 5036 515 39 601 Fragments arranged by size: 5036 601 515 319 39 BsmI GAATG_Cn' Cuts at: 0 705 1653 5947 6510 Size: 705 948 4294 563 Fragments arranged by size: 4294 948 705 563 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1531 6510 Size: 1531 4979 Bsp1286I G_dGCh'C Cuts at: 0 20 319 469 1718 2890 3228 3512 3956 Size: 20 299 150 1249 1172 338 284 444 Cuts at: 3956 4265 4724 5029 5355 5870 5909 6510 Size: 309 459 305 326 515 39 601 Fragments arranged by size: 1249 1172 601 515 459 444 338 326 309 305 299 284 150 39 20 BspHI T'CATG_A Cuts at: 0 756 4692 5439 6510 Size: 756 3936 747 1071 Fragments arranged by size: 3936 1071 756 747 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1049 3088 4189 6510 Size: 1049 2039 1101 2321 Fragments arranged by size: 2321 2039 1101 1049 BsrDI GCAATG_nn' Cuts at: 0 49 1104 2414 3035 5876 6510 Size: 49 1055 1310 621 2841 634 Fragments arranged by size: 2841 1310 1055 634 621 49 BsrFI r'CCGG_y Cuts at: 0 4344 6510 Size: 4344 2166 BsrGI T'GTAC_A Cuts at: 0 3966 6510 Size: 3966 2544 BssSI C'ACGA_G Cuts at: 0 2291 3122 6510 Size: 2291 831 3388 Fragments arranged by size: 3388 2291 831 BstAPI GCAn_nnn'nTGC Cuts at: 0 2404 4465 4482 6510 Size: 2404 2061 17 2028 Fragments arranged by size: 2404 2061 2028 17 BstEII G'GTnAC_C Cuts at: 0 733 2428 6510 Size: 733 1695 4082 Fragments arranged by size: 4082 1695 733 BstXI CCAn_nnnn'nTGG Cuts at: 0 1228 4014 6510 Size: 1228 2786 2496 Fragments arranged by size: 2786 2496 1228 BstYI r'GATC_y Cuts at: 0 4209 5010 5372 5978 6510 Size: 4209 801 362 606 532 Fragments arranged by size: 4209 801 606 532 362 Bsu36I CC'TnA_GG Cuts at: 0 1958 3083 4451 6510 Size: 1958 1125 1368 2059 Fragments arranged by size: 2059 1958 1368 1125 BtgI C'CryG_G Cuts at: 0 710 866 3512 3581 4025 5756 6510 Size: 710 156 2646 69 444 1731 754 Fragments arranged by size: 2646 1731 754 710 444 156 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1091 6510 Size: 1091 5419 BtsI GCAGTG_nn' Cuts at: 0 1187 1355 2405 4154 4554 5117 5163 6510 Size: 1187 168 1050 1749 400 563 46 1347 Fragments arranged by size: 1749 1347 1187 1050 563 400 168 46 ClaI AT'CG_AT Cuts at: 0 155 6510 Size: 155 6355 DraI TTT'AAA Cuts at: 0 2868 4167 6309 6345 6510 Size: 2868 1299 2142 36 165 Fragments arranged by size: 2868 2142 1299 165 36 DraIII CAC_nnn'GTG Cuts at: 0 3232 6201 6510 Size: 3232 2969 309 Fragments arranged by size: 3232 2969 309 DrdI GACnn_nn'nnGTC Cuts at: 0 2601 2616 6510 Size: 2601 15 3894 Fragments arranged by size: 3894 2601 15 EaeI y'GGCC_r Cuts at: 0 869 2781 3745 4346 5844 6510 Size: 869 1912 964 601 1498 666 Fragments arranged by size: 1912 1498 964 869 666 601 EarI CTCTTCn'nnn_ Cuts at: 0 1079 3305 3362 3653 3944 4806 5300 5343 Size: 1079 2226 57 291 291 862 494 43 Cuts at: 5343 5666 6150 6510 Size: 323 484 360 Fragments arranged by size: 2226 1079 862 494 484 360 323 291 291 57 43 EcoICRI GAG'CTC Cuts at: 0 5353 5907 6510 Size: 5353 554 603 Fragments arranged by size: 5353 603 554 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 617 798 1000 1307 1965 2286 2597 Size: 505 112 181 202 307 658 321 311 Cuts at: 2597 3286 3341 3727 4036 4109 4354 4862 5339 Size: 689 55 386 309 73 245 508 477 Cuts at: 5339 5645 6011 6510 Size: 306 366 499 Fragments arranged by size: 689 658 508 505 499 477 386 366 321 311 309 307 306 245 202 181 112 73 55 EcoNI CCTnn'n_nnAGG Cuts at: 0 6005 6510 Size: 6005 505 EcoO109I rG'GnC_Cy Cuts at: 0 16 17 26 1300 5099 6481 6510 Size: 16 1 9 1274 3799 1382 29 Fragments arranged by size: 3799 1382 1274 29 16 9 1 EcoRI G'AATT_C Cuts at: 0 184 1093 1391 2745 4508 5680 6510 Size: 184 909 298 1354 1763 1172 830 Fragments arranged by size: 1763 1354 1172 909 830 298 184 EcoRV GAT'ATC Cuts at: 0 3648 6510 Size: 3648 2862 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1699 1763 3232 4001 6510 Size: 1699 64 1469 769 2509 Fragments arranged by size: 2509 1699 1469 769 64 HaeII r_GCGC'y Cuts at: 0 3558 4289 6510 Size: 3558 731 2221 Fragments arranged by size: 3558 2221 731 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 304 336 1303 1334 1335 1366 1849 1881 Size: 304 32 967 31 1 31 483 32 Cuts at: 1881 1976 2008 2236 2268 2632 2664 2877 2909 Size: 95 32 228 32 364 32 213 32 Cuts at: 2909 3007 3039 3301 3333 3702 3734 3895 3927 Size: 98 32 262 32 369 32 161 32 Cuts at: 3927 4054 4086 5461 5493 6510 Size: 127 32 1375 32 1017 Fragments arranged by size: 1375 1017 967 483 369 364 304 262 228 213 161 127 98 95 32 32 32 32 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 0 4107 4665 6510 Size: 4107 558 1845 Fragments arranged by size: 4107 1845 558 HpaI GTT'AAC Cuts at: 0 4107 6510 Size: 4107 2403 KasI G'GCGC_C Cuts at: 0 4285 6510 Size: 4285 2225 KpnI G_GTAC'C Cuts at: 0 1682 6510 Size: 1682 4828 MfeI C'AATT_G Cuts at: 0 334 2939 6510 Size: 334 2605 3571 Fragments arranged by size: 3571 2605 334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 200 352 527 1756 3149 3483 6510 Size: 200 152 175 1229 1393 334 3027 Fragments arranged by size: 3027 1393 1229 334 200 175 152 MscI TGG'CCA Cuts at: 0 871 2783 3747 5846 6510 Size: 871 1912 964 2099 664 Fragments arranged by size: 2099 1912 964 871 664 MslI CAynn'nnrTG Cuts at: 0 547 668 1310 1790 3098 5110 5812 5852 Size: 547 121 642 480 1308 2012 702 40 Cuts at: 5852 6510 Size: 658 Fragments arranged by size: 2012 1308 702 658 642 547 480 121 40 MspA1I CmG'CkG Cuts at: 0 33 481 1546 4075 5623 5640 6510 Size: 33 448 1065 2529 1548 17 870 Fragments arranged by size: 2529 1548 1065 870 448 33 17 NaeI GCC'GGC Cuts at: 0 4346 6510 Size: 4346 2164 NarI GG'CG_CC Cuts at: 0 4286 6510 Size: 4286 2224 NcoI C'CATG_G Cuts at: 0 710 6510 Size: 710 5800 NgoMIV G'CCGG_C Cuts at: 0 4344 6510 Size: 4344 2166 NsiI A_TGCA'T Cuts at: 0 2106 6510 Size: 2106 4404 NspI r_CATG'y Cuts at: 0 1060 1905 2779 3097 6243 6510 Size: 1060 845 874 318 3146 267 Fragments arranged by size: 3146 1060 874 845 318 267 PasI CC'CwG_GG Cuts at: 0 5031 6510 Size: 5031 1479 PflMI CCAn_nnn'nTGG Cuts at: 0 528 2050 3339 4878 5158 6510 Size: 528 1522 1289 1539 280 1352 Fragments arranged by size: 1539 1522 1352 1289 528 280 PfoI T'CCnGG_A Cuts at: 0 1386 4013 6510 Size: 1386 2627 2497 Fragments arranged by size: 2627 2497 1386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3100 3132 5768 5800 6510 Size: 3100 32 2636 32 710 Fragments arranged by size: 3100 2636 710 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1300 5099 6510 Size: 1300 3799 1411 Fragments arranged by size: 3799 1411 1300 PshAI GACnn'nnGTC Cuts at: 0 3574 3791 6510 Size: 3574 217 2719 Fragments arranged by size: 3574 2719 217 PsiI TTA'TAA Cuts at: 0 440 3715 6510 Size: 440 3275 2795 Fragments arranged by size: 3275 2795 440 PspOMI G'GGCC_C Cuts at: 0 16 6510 Size: 16 6494 PstI C_TGCA'G Cuts at: 0 3263 3289 5009 6510 Size: 3263 26 1720 1501 Fragments arranged by size: 3263 1720 1501 26 PvuII CAG'CTG Cuts at: 0 481 1546 5623 5640 6510 Size: 481 1065 4077 17 870 Fragments arranged by size: 4077 1065 870 481 17 SacI G_AGCT'C Cuts at: 0 5355 5909 6510 Size: 5355 554 601 Fragments arranged by size: 5355 601 554 SanDI GG'GwC_CC Cuts at: 0 5099 6510 Size: 5099 1411 SapI GCTCTTCn'nnn_ Cuts at: 0 3944 5343 6510 Size: 3944 1399 1167 Fragments arranged by size: 3944 1399 1167 ScaI AGT'ACT Cuts at: 0 2676 6510 Size: 2676 3834 SexAI A'CCwGG_T Cuts at: 0 4816 6510 Size: 4816 1694 SfcI C'TryA_G Cuts at: 0 2510 3259 3285 4034 4205 5005 6080 6510 Size: 2510 749 26 749 171 800 1075 430 Fragments arranged by size: 2510 1075 800 749 749 430 171 26 SfiI GGCCn_nnn'nGGCC Cuts at: 0 24 6510 Size: 24 6486 SfoI GGC'GCC Cuts at: 0 4287 6510 Size: 4287 2223 SmaI CCC'GGG Cuts at: 0 464 3991 6510 Size: 464 3527 2519 Fragments arranged by size: 3527 2519 464 SmlI C'TyrA_G Cuts at: 0 2268 2495 4141 5860 6150 6510 Size: 2268 227 1646 1719 290 360 Fragments arranged by size: 2268 1719 1646 360 290 227 SphI G_CATG'C Cuts at: 0 1905 3097 6510 Size: 1905 1192 3413 Fragments arranged by size: 3413 1905 1192 SspI AAT'ATT Cuts at: 0 2488 6510 Size: 2488 4022 StyI C'CwwG_G Cuts at: 0 710 2282 2383 2433 2735 2953 4955 6510 Size: 710 1572 101 50 302 218 2002 1555 Fragments arranged by size: 2002 1572 1555 710 302 218 101 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 562 1102 1106 3068 3243 6189 6510 Size: 562 540 4 1962 175 2946 321 Fragments arranged by size: 2946 1962 562 540 321 175 4 TatI w'GTAC_w Cuts at: 0 795 1666 2674 2772 3966 4238 4828 6510 Size: 795 871 1008 98 1194 272 590 1682 Fragments arranged by size: 1682 1194 1008 871 795 590 272 98 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 503 535 2356 2388 3239 3271 3440 3472 Size: 503 32 1821 32 851 32 169 32 Cuts at: 3472 6095 6127 6510 Size: 2623 32 383 Fragments arranged by size: 2623 1821 851 503 383 169 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1861 2250 3811 5118 6510 Size: 1861 389 1561 1307 1392 Fragments arranged by size: 1861 1561 1392 1307 389 XbaI T'CTAG_A Cuts at: 0 3720 6510 Size: 3720 2790 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 128 2757 6214 6510 Size: 128 2629 3457 296 Fragments arranged by size: 3457 2629 296 128 XmaI C'CCGG_G Cuts at: 0 462 3989 6510 Size: 462 3527 2521 Fragments arranged by size: 3527 2521 462 XmnI GAAnn'nnTTC Cuts at: 0 1320 3981 4442 4508 5783 6510 Size: 1320 2661 461 66 1275 727 Fragments arranged by size: 2661 1320 1275 727 461 66 ZraI GAC'GTC Cuts at: 0 4698 6510 Size: 4698 1812 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AgeI AhdI AscI AseI AsiSI AvrII BaeI BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssHII BstBI BstZ17I CspCI EagI EciI FseI FspI FspAI HindIII MluI NdeI NheI NotI NruI PacI PciI PmeI PmlI PspXI PsrI PvuI RsrII SacII SalI SbfI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI XhoI