################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AlwNI 498 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 343 GAGCTC G[AT]GC[AT]^C AvaI 30 CCCGGG C^[CT]CG[GA]G BalI 228 TGGCCA TGG^CCA BanII 343 GAGCTC G[GA]GC[CT]^C BmyI 343 GAGCTC G[AGT]GC[ACT]^C BsaI 481 GAGACC GGTCTC(1/5) BsiHKAI 343 GAGCTC G[AT]GC[AT]^C BsmAI 481 GAGAC GTCTC(1/5) BsoBI 30 CCCGGG C^[CT]CG[GA]G Bsp1286I 343 GAGCTC G[AGT]GC[ACT]^C BspHI 144 TCATGA T^CATGA BsrDI 103 GCAATG GCAATG(2/0) BstAPI 258 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 563 CATCC GGATG(2/0) DpnII 406 GATC ^GATC DraI 337 TTTAAA TTT^AAA EaeI 228 TGGCCA [CT]^GGCC[GA] EcoRI 238 GAATTC G^AATTC FokI 563 CATCC GGATG(9/13) HaeIII 229 GGCC GG^CC HapII 31 CCGG C^CGG HpaII 31 CCGG C^CGG MboI 406 GATC ^GATC MluNI 228 TGGCCA TGG^CCA MscI 228 TGGCCA TGG^CCA MseI 338 TTAA T^TAA MspA1I 21 CAGCTG C[AC]G^C[GT]G MspI 31 CCGG C^CGG NdeII 406 GATC ^GATC NlaIV 49 GGGTCC GG[ACGT]^[ACGT]CC NspI 263 ACATGC [GA]CATG^[CT] PshAI 174 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 488 CTGCAG CTGCA^G PvuII 21 CAGCTG CAG^CTG RcaI 144 TCATGA T^CATGA SacI 343 GAGCTC GAGCT^C Sau3AI 406 GATC ^GATC SmaI 30 CCCGGG CCC^GGG SmlI 444 CTTGAG C^T[CT][GA]AG TfiI 500 GATTC G^A[AT]TC Tru9I 338 TTAA T^TAA Tsp45I 301 GTCAC ^GT[GC]AC XmaCI 30 CCCGGG C^CCGGG XmaI 30 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 60 GTAC GT^AC 261 GTAC AvaII 50 GGTCC G^G[AT]CC 357 GGTCC BcnI 30 CCCGG CC^[GC]GG 31 CCGGG BsiYI 176 CCCAGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 381 CCTGGTGCTGG BslI 176 CCCAGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 381 CCTGGTGCTGG CfoI 38 GCGC GCG^C 101 GCGC Cfr13I 50 GGTCC G^G[ACGT]CC 357 GGTCC DdeI 496 CTCAG C^T[ACGT]AG 528 CTTAG EarI 585 GAAGAG CTCTTC(1/4) 633 GAAGAG FauI 47 GCGGG CCCGC(4/6) 252 GCGGG HhaI 38 GCGC GCG^C 101 GCGC HinP1I 38 GCGC G^CGC 101 GCGC Ksp632I 585 GAAGAG CTCTTC(1/4) 633 GAAGAG MaeIII 301 GTCAC ^GT[ACGT]AC 395 GTAAC MslI 243 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 364 CACACCTATG NciI 30 CCCGG CC^[GC]GG 31 CCGGG PleI 447 GAGTC GAGTC(4/5) 553 GAGTC RsaI 60 GTAC GT^AC 261 GTAC Sau96I 50 GGTCC G^G[ACGT]CC 357 GGTCC TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^ 303 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BsaJI BssKI BstNI Cac8I EcoRII Fnu4HI HinfI ItaI MboII MnlI MvaI MwoI NlaIII ScrFI SfaNI SfcI TseI Tsp509I