################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AviII AvrII BalI BbeI BbsI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 29 ACGTGT A^C[GA][CT]GT ApaI 79 GGGCCC GGGCC^C AseI 1339 ATTAAT AT^TAAT AsnI 1339 ATTAAT AT^TAAT AspI 1177 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 176 CCCGAG C^[CT]CG[GA]G BamHI 1188 GGATCC G^GATCC BanI 6 GGCACC G^G[CT][GA]CC BbrPI 28 CACGTG CAC^GTG BbvCI 1748 CCTCAGC CCTCAGC(-5/-2) BcgI 178 CGAGTCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1932 GGATAC GTATCC(6/5) BcnI 48 CCGGG CC^[GC]GG BglII 660 AGATCT A^GATCT BsmFI 1495 GTCCC GGGAC(10/14) BsoBI 176 CCCGAG C^[CT]CG[GA]G Bsp1407I 379 TGTACA T^GTACA BspHI 843 TCATGA T^CATGA BspMI 1599 GCAGGT ACCTGC(4/8) BsrFI 838 GCCGGT [GA]^CCGG[CT] BsrGI 379 TGTACA T^GTACA BstXI 1123 CCAAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 838 GCCGGT [GA]^CCGG[CT] DraII 321 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 2440 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 321 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 557 GAATTC G^AATTC FauI 54 GCGGG CCCGC(4/6) HgaI 2180 GCGTC GACGC(5/10) MfeI 183 CAATTG C^AATTG MunI 183 CAATTG C^AATTG NciI 48 CCGGG CC^[GC]GG NheI 834 GCTAGC G^CTAGC PflFI 1177 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 28 CACGTG CAC^GTG PmlI 28 CACGTG CAC^GTG PpuMI 321 AGGACCT [GA]G^G[AT]CC[CT] PshBI 1339 ATTAAT AT^TAAT RcaI 843 TCATGA T^CATGA SacI 72 GAGCTC GAGCT^C ScaI 1579 AGTACT AGT^ACT SexAI 1909 ACCTGGT A^CC[AT]GGT SfaNI 303 GCATC GCATC(5/9) SpeI 1576 ACTAGT A^CTAGT SspBI 379 TGTACA T^GTACA StuI 1297 AGGCCT AGG^CCT Tth111I 1177 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 83 CCAGAGGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 1999 GTGCAC G^TGCAC 2471 GTGCAC ApaLI 1999 GTGCAC G^TGCAC 2471 GTGCAC Asp700I 988 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1372 GAAAAAGTTC BsaAI 28 CACGTG [CT]AC^GT[GA] 2212 TACGTG BsaBI 917 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1468 GATAGTGATC BsgI 1394 CTGCAC GTGCAG(16/14) 1819 GTGCAG BstF5I 210 GGATG GGATG(2/0) 744 GGATG DraI 291 TTTAAA TTT^AAA 462 TTTAAA EcoT14I 932 CCATGG C^C[AT][AT]GG 1249 CCATGG FokI 210 GGATG GGATG(9/13) 744 GGATG HapII 48 CCGG C^CGG 839 CCGG HpaI 1117 GTTAAC GTT^AAC 1572 GTTAAC HpaII 48 CCGG C^CGG 839 CCGG MamI 917 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1468 GATAGTGATC MspA1I 398 CAGCTG C[AC]G^C[GT]G 2171 CAGCTG MspI 48 CCGG C^CGG 839 CCGG NcoI 932 CCATGG C^CATGG 1249 CCATGG PshAI 419 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2169 GACAGCTGTC PvuII 398 CAGCTG CAG^CTG 2171 CAGCTG SphI 339 GCATGC GCATG^C 1776 GCATGC SspI 528 AATATT AAT^ATT 1707 AATATT StyI 932 CCATGG C^C[AT][AT]GG 1249 CCATGG XmnI 988 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1372 GAAAAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbvI BclI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstAPI BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII FbaI Fnu4HI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII