Restriction map for
pFN21AE3412
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspA1I MunI MvnI
NarI NciI NcoI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 153 CCGC CCGC(-3/-1)
AfaI 951 GTAC GT^AC
AflII 549 CTTAAG C^TTAAG
Alw44I 253 GTGCAC G^TGCAC
ApaI 193 GGGCCC GGGCC^C
ApaLI 253 GTGCAC G^TGCAC
Asp700I 728 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 406 GGACC G^G[AT]CC
BalI 302 TGGCCA TGG^CCA
BbsI 565 GAAGAC GAAGAC(2/6)
BbvCI 240 GCTGAGG CCTCAGC(-5/-2)
BfrI 549 CTTAAG C^TTAAG
BglII 416 AGATCT A^GATCT
BlpI 788 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 788 GCTCAGC GC^T[ACGT]AGC
BpuAI 565 GAAGAC GAAGAC(2/6)
BsaI 145 GAGACC GGTCTC(1/5)
BseRI 49 GAGGAG GAGGAG(10/8)
BsgI 334 GTGCAG GTGCAG(16/14)
BsmI 859 GCATTC GAATGC(1/-1)
BsrFI 212 GCCGGC [GA]^CCGG[CT]
BsrI 122 ACTGG ACTGG(1/-1)
CelII 788 GCTCAGC GC^T[ACGT]AGC
CfoI 872 GCGC GCG^C
Cfr10I 212 GCCGGC [GA]^CCGG[CT]
DraII 193 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 302 TGGCCA [CT]^GGCC[GA]
EcoO109I 193 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 260 CCAAGG C^C[AT][AT]GG
EcoT22I 836 ATGCAT ATGCA^T
HhaI 872 GCGC GCG^C
HinP1I 872 GCGC G^CGC
HphI 187 TCACC GGTGA(8/7)
MluNI 302 TGGCCA TGG^CCA
MscI 302 TGGCCA TGG^CCA
MseI 550 TTAA T^TAA
NaeI 212 GCCGGC GCC^GGC
NdeI 655 CATATG CA^TATG
NgoMIV 212 GCCGGC G^CCGGC
NheI 456 GCTAGC G^CTAGC
NsiI 836 ATGCAT ATGCA^T
PflMI 349 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 109 GACTC GAGTC(4/5)
RsaI 951 GTAC GT^AC
SacI 786 GAGCTC GAGCT^C
SapI 88 GAAGAGC GCTCTTC(1/4)
SfcI 135 CTACAG C^T[GA][CT]AG
SmlI 549 CTTAAG C^T[CT][GA]AG
StuI 326 AGGCCT AGG^CCT
StyI 260 CCAAGG C^C[AT][AT]GG
TaqI 891 TCGA T^CGA
Tru9I 550 TTAA T^TAA
TthHB8I 891 TCGA T^CGA
Van91I 349 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 180 CCAGGATTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 728 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 103 GATCC GGATC(4/5)
270 GATCC
AlwNI 268 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
295 CAGTTCCTG
AspHI 253 GTGCAC G[AT]GC[AT]^C
786 GAGCTC
BanII 193 GGGCCC G[GA]GC[CT]^C
786 GAGCTC
BsiHKAI 253 GTGCAC G[AT]GC[AT]^C
786 GAGCTC
BsiYI 213 CCGGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
349 CCAGAGAATGG
BslI 213 CCGGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
349 CCAGAGAATGG
BsmAI 145 GAGAC GTCTC(1/5)
720 GAGAC
BsrDI 631 CATTGC GCAATG(2/0)
635 GCAATG
BstYI 269 AGATCC [GA]^GATC[CT]
416 AGATCT
EarI 82 GAAGAG CTCTTC(1/4)
88 GAAGAG
HapII 213 CCGG C^CGG
247 CCGG
HpaII 213 CCGG C^CGG
247 CCGG
Ksp632I 82 GAAGAG CTCTTC(1/4)
88 GAAGAG
MflI 269 AGATCC [GA]^GATC[CT]
416 AGATCT
MspI 213 CCGG C^CGG
247 CCGG
NspI 838 GCATGT [GA]CATG^[CT]
921 ACATGC
XhoII 269 AGATCC [GA]^GATC[CT]
416 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI BbvI BfaI BmyI BpmI
BsaJI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp45I Tsp509I TspRI