Restriction map for
pFN21AE3412
(Circular) (Six-base) MAPSORT of: pFN21AE3412.seq Check: 1248 from: 1 to: 5704
pFN21AE3412 5704 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2312 2454 2531 2993 3767 5151 5419
Size: 402 278 142 77 462 774 1384 268
Cuts at: 5419 1632
Size: 1917
Fragments arranged by size:
1917 1384 774 462 402 278 268 142
77
AflII C'TTAA_G
Cuts at: 820 1017 2554 820
Size: 197 1537 3970
Fragments arranged by size:
3970 1537 197
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2648 1184
Size: 1464 4240
AlwNI CAG_nnn'CTG
Cuts at: 2278 2305 3447 5019 2278
Size: 27 1142 1572 2963
Fragments arranged by size:
2963 1572 1142 27
ApaI G_GGCC'C
Cuts at: 2202 2202
Size: 5704
ApaLI G'TGCA_C
Cuts at: 2258 3761 4917 2258
Size: 1503 1156 3045
Fragments arranged by size:
3045 1503 1156
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3025 4480 618
Size: 325 217 627 1238 1455 1842
Fragments arranged by size:
1842 1455 1238 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2795 3023 1391
Size: 811 593 228 4072
Fragments arranged by size:
4072 811 593 228
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2577 928
Size: 971 678 4055
Fragments arranged by size:
4055 971 678
BbvCI CC'TCA_GC
Cuts at: 2246 2246
Size: 5704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BfrBI ATG'CAT
Cuts at: 2843 2843
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4315 136
Size: 107 122 71 631 605 2643 1525
Fragments arranged by size:
2643 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2421 5699 2421
Size: 3278 2426
BlpI GC'TnA_GC
Cuts at: 2794 3121 2794
Size: 327 5377
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2262 2981 3765 4921 1790
Size: 412 60 719 784 1156 2573
Fragments arranged by size:
2573 1156 784 719 412 60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2465 1056
Size: 1409 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2300 2315 2405 2510 4364 1239
Size: 910 151 15 90 105 1854 2579
Fragments arranged by size:
2579 1854 910 151 105 90 15
Bpu10I CC'TnA_GC
Cuts at: 2246 2246
Size: 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 4367 882
Size: 617 645 2223 2219
Fragments arranged by size:
2223 2219 645 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3511 3893 4528 275
Size: 53 83 186 936 1978 382 635 1451
Fragments arranged by size:
1978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 2988 3018 1178
Size: 30 165 30 1131 30 424 30 3864
Fragments arranged by size:
3864 1131 424 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 4821
BseYI C'CCAG_C
Cuts at: 4907 5403 4907
Size: 496 5208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 1163
Size: 573 624 4507
Fragments arranged by size:
4507 624 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2795 3765 3850 4921 2262
Size: 533 970 85 1071 3045
Fragments arranged by size:
3045 1071 970 533 85
BsmI GAATG_Cn'
Cuts at: 2864 3215 3308 2864
Size: 351 93 5260
Fragments arranged by size:
5260 351 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2262 2795 2981 3023 3765 3850
Size: 399 412 60 533 186 42 742 85
Cuts at: 3850 4921 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 533 412 399 186 85
60 42
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2635 2647 4199 4373 65
Size: 1183 1387 12 1552 174 1396
Fragments arranged by size:
1552 1396 1387 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 4348 4535 1700
Size: 110 407 2131 187 2869
Fragments arranged by size:
2869 2131 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5704
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2274 2421 3034 3401 3786 3803 5255 5341
Size: 302 147 613 367 385 17 1452 86
Cuts at: 5341 5353 5699 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 613 385 367 346 302 147
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 3151 4040
Size: 54 1425 50 275 18 477 844 889
Cuts at: 4040 5669 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 844 477 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2088 3634 1353
Size: 729 6 1546 3423
Fragments arranged by size:
3423 1546 729 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 4291 4665 4811 387
Size: 1064 138 558 2144 374 146 1280
Fragments arranged by size:
2144 1280 1064 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 2793 2793
Size: 5704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2149 2300 2312 2315 2405 2454
Size: 393 402 115 151 12 3 90 49
Cuts at: 2454 2510 2531 2993 3767 4364 5151 5419 1239
Size: 56 21 462 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 597 462 402 393 268
151 115 90 56 49 21 12 3
EcoO109I rG'GnC_Cy
Cuts at: 2199 2199
Size: 5704
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2099 2124 2131 2534
Size: 32 135 32 520 7 25 7 403
Cuts at: 2534 2566 2878 2910 4345 4377 4419 4451 1373
Size: 32 312 32 1435 32 42 32 2626
Fragments arranged by size:
2626 1435 520 403 312 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 10
Size: 54 1425 343 477 3405
Fragments arranged by size:
3405 1425 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 2640 2664 3662 4021 4180 518
Size: 648 1344 130 24 998 359 159 2042
Fragments arranged by size:
2042 1344 998 648 359 159 130 24
MspA1I CmG'CkG
Cuts at: 1606 3120 3797 4945 5190 1606
Size: 1514 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 245
NaeI GCC'GGC
Cuts at: 2219 2219
Size: 5704
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5152
NdeI CA'TA_TG
Cuts at: 387 2661 387
Size: 2274 3430
NgoMIV G'CCGG_C
Cuts at: 2217 2217
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 2461 1052
Size: 1409 4295
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NsiI A_TGCA'T
Cuts at: 2845 2845
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2847 2930 3062 4607 1132
Size: 97 1618 83 132 1545 2229
Fragments arranged by size:
2229 1618 1545 132 97 83
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 4452
PfoI T'CCnGG_A
Cuts at: 2277 2277
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2198 2198
Size: 5704
PstI C_TGCA'G
Cuts at: 830 3056 830
Size: 2226 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SacI G_AGCT'C
Cuts at: 2795 2795
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SapI GCTCTTCn'nnn_
Cuts at: 2088 2088
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2140 3052 4187 4868 5059 826
Size: 219 1095 912 1135 681 191 1471
Fragments arranged by size:
1471 1135 1095 912 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2554 3808 4709 4971 820
Size: 197 926 611 1254 901 262 1553
Fragments arranged by size:
1553 1254 926 901 611 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2333 1517
Size: 816 4888
StyI C'CwwG_G
Cuts at: 513 1065 2265 3143 513
Size: 552 1200 878 3074
Fragments arranged by size:
3074 1200 878 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2326 3731 3748 3901 4086 4531 325
Size: 1255 746 1405 17 153 185 445 1498
Fragments arranged by size:
1498 1405 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2955 3950 5662
Size: 274 80 33 51 1443 978 995 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1443 995 978 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2192
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2737 3833 2737
Size: 1096 4608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NarI NruI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI