Restriction map for
pFN21AE3412
(Linear) (Six-base) MAPSORT of: KIEE3412.seq Check: 8800 from: 1 to: 999
KIEE3412.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 307 449 526 988 999
Size: 29 278 142 77 462 11
Fragments arranged by size:
462 278 142 77 29 11
AflII C'TTAA_G
Cuts at: 0 549 999
Size: 549 450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 643 999
Size: 643 356
AlwNI CAG_nnn'CTG
Cuts at: 0 273 300 999
Size: 273 27 699
Fragments arranged by size:
699 273 27
ApaI G_GGCC'C
Cuts at: 0 197 999
Size: 197 802
ApaLI G'TGCA_C
Cuts at: 0 253 999
Size: 253 746
BanII G_rGCy'C
Cuts at: 0 197 790 999
Size: 197 593 209
Fragments arranged by size:
593 209 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 572 999
Size: 572 427
BbvCI CC'TCA_GC
Cuts at: 0 241 999
Size: 241 758
BfrBI ATG'CAT
Cuts at: 0 838 999
Size: 838 161
BglII A'GATC_T
Cuts at: 0 416 999
Size: 416 583
BlpI GC'TnA_GC
Cuts at: 0 789 999
Size: 789 210
Bme1580I G_kGCm'C
Cuts at: 0 197 257 976 999
Size: 197 60 719 23
Fragments arranged by size:
719 197 60 23
BmtI G_CTAG'C
Cuts at: 0 460 999
Size: 460 539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 295 310 400 505 999
Size: 144 151 15 90 105 494
Fragments arranged by size:
494 151 144 105 90 15
Bpu10I CC'TnA_GC
Cuts at: 0 241 999
Size: 241 758
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 999
Size: 139 860
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 999
Size: 529 30 440
Fragments arranged by size:
529 440 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 999
Size: 64 935
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 999
Size: 355 644
BsiHKAI G_wGCw'C
Cuts at: 0 257 790 999
Size: 257 533 209
Fragments arranged by size:
533 257 209
BsmI GAATG_Cn'
Cuts at: 0 859 999
Size: 859 140
Bsp1286I G_dGCh'C
Cuts at: 0 197 257 790 976 999
Size: 197 60 533 186 23
Fragments arranged by size:
533 197 186 60 23
BsrDI GCAATG_nn'
Cuts at: 0 630 642 999
Size: 630 12 357
Fragments arranged by size:
630 357 12
BsrFI r'CCGG_y
Cuts at: 0 212 999
Size: 212 787
BstYI r'GATC_y
Cuts at: 0 269 416 999
Size: 269 147 583
Fragments arranged by size:
583 269 147
EaeI y'GGCC_r
Cuts at: 0 302 999
Size: 302 697
EarI CTCTTCn'nnn_
Cuts at: 0 77 83 999
Size: 77 6 916
Fragments arranged by size:
916 77 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 999
Size: 142 857
EcoICRI GAG'CTC
Cuts at: 0 788 999
Size: 788 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 144 295 307 310 400 449 505
Size: 29 115 151 12 3 90 49 56
Cuts at: 505 526 988 999
Size: 21 462 11
Fragments arranged by size:
462 151 115 90 56 49 29 21
12 11 3
EcoO109I rG'GnC_Cy
Cuts at: 0 194 999
Size: 194 805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 435 999
Size: 435 564
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 94 119 126 529 561 873 905
Size: 87 7 25 7 403 32 312 32
Cuts at: 905 999
Size: 94
Fragments arranged by size:
403 312 94 87 32 32 25 7
7
MscI TGG'CCA
Cuts at: 0 304 999
Size: 304 695
MslI CAynn'nnrTG
Cuts at: 0 505 635 659 999
Size: 505 130 24 340
Fragments arranged by size:
505 340 130 24
NaeI GCC'GGC
Cuts at: 0 214 999
Size: 214 785
NdeI CA'TA_TG
Cuts at: 0 656 999
Size: 656 343
NgoMIV G'CCGG_C
Cuts at: 0 212 999
Size: 212 787
NheI G'CTAG_C
Cuts at: 0 456 999
Size: 456 543
NsiI A_TGCA'T
Cuts at: 0 840 999
Size: 840 159
NspI r_CATG'y
Cuts at: 0 842 925 999
Size: 842 83 74
Fragments arranged by size:
842 83 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 999
Size: 355 644
PfoI T'CCnGG_A
Cuts at: 0 272 999
Size: 272 727
PspOMI G'GGCC_C
Cuts at: 0 193 999
Size: 193 806
SacI G_AGCT'C
Cuts at: 0 790 999
Size: 790 209
SapI GCTCTTCn'nnn_
Cuts at: 0 83 999
Size: 83 916
SfcI C'TryA_G
Cuts at: 0 135 999
Size: 135 864
SmlI C'TyrA_G
Cuts at: 0 549 999
Size: 549 450
StuI AGG'CCT
Cuts at: 0 328 999
Size: 328 671
StyI C'CwwG_G
Cuts at: 0 260 999
Size: 260 739
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 999
Size: 321 678
TatI w'GTAC_w
Cuts at: 0 950 999
Size: 950 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 999
Size: 187 812
XmnI GAAnn'nnTTC
Cuts at: 0 732 999
Size: 732 267
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI ApaLI BanII BbsI
BbvCI BfrBI BglII BlpI Bme1580I BmtI BpmI Bpu10I
BsaI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BstYI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI Hin4I MscI MslI NaeI NdeI NgoMIV NheI
NsiI NspI PflMI PfoI PspOMI SacI SapI SfcI
SmlI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BglI BmgBI BmrI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NarI NcoI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI