################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI KspI MaeI MfeI MflI MluI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 143 TCCGGA T^CCGGA AcyI 36 GGCGCC G[GA]^CG[CT]C AfaI 363 GTAC GT^AC AgeI 656 ACCGGT A^CCGGT AlwI 462 GGATC GGATC(4/5) AlwNI 254 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 59 GGGCCC GGGCC^C Asp700I 244 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 15 GAGCAC G[AT]GC[AT]^C BalI 156 TGGCCA TGG^CCA BanI 36 GGCGCC G^G[CT][GA]CC BbeI 36 GGCGCC GGCGC^C BbsI 448 GAAGAC GAAGAC(2/6) BlpI 12 GCTGAGC GC^T[ACGT]AGC BpmI 64 CTGGAG CTGGAG(16/14) Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC BpuAI 448 GAAGAC GAAGAC(2/6) BsaAI 160 CACGTA [CT]AC^GT[GA] BsaBI 532 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 36 GGCGCC G[GA]^CG[CT]C BseAI 143 TCCGGA T^CCGGA BseRI 397 GAGGAG GAGGAG(10/8) BsiHKAI 15 GAGCAC G[AT]GC[AT]^C BsmAI 400 GAGAC GTCTC(1/5) BspEI 143 TCCGGA T^CCGGA BsrFI 656 ACCGGT [GA]^CCGG[CT] BsrI 188 CCAGT ACTGG(1/-1) CelII 12 GCTGAGC GC^T[ACGT]AGC Cfr10I 656 ACCGGT [GA]^CCGG[CT] EaeI 156 TGGCCA [CT]^GGCC[GA] EarI 644 GAAGAG CTCTTC(1/4) EcoNI 238 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 36 GGCGCC [GA]GCGC^[CT] Hin1I 36 GGCGCC G[GA]^CG[CT]C KasI 36 GGCGCC G^GCGCC Ksp632I 644 GAAGAG CTCTTC(1/4) MamI 532 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 156 TGGCCA TGG^CCA MroI 143 TCCGGA T^CCGGA MscI 156 TGGCCA TGG^CCA NarI 36 GGCGCC GG^CGCC PinAI 656 ACCGGT A^CCGGT PleI 647 GAGTC GAGTC(4/5) PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT] RsaI 363 GTAC GT^AC SfoI 36 GGCGCC GGC^GCC StuI 496 AGGCCT AGG^CCT Tsp509I 624 AATT ^AATT XmnI 244 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 54 CGCG CG^CG 56 CGCG BanII 3 GGGCTC G[GA]GC[CT]^C 59 GGGCCC BbvI 406 GCAGC GCAGC(8/12) 545 GCAGC BcnI 81 CCCGG CC^[GC]GG 225 CCGGG BsaJI 62 CCCTGG C^C[ACGT][ACGT]GG 314 CCCTGG BsaWI 143 TCCGGA [AT]^CCGG[AT] 656 ACCGGT BsiYI 50 CCACCGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 238 CCTTCAGAAGG BslI 50 CCACCGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 238 CCTTCAGAAGG BsmFI 77 GGGAC GGGAC(10/14) 422 GTCCC BstNI 63 CCTGG CC^[AT]GG 315 CCTGG BstUI 54 CGCG CG^CG 56 CGCG DpnII 463 GATC ^GATC 485 GATC DraII 59 GGGCCCT [GA]G^G[ACGT]CC[CT] 77 GGGACCC EcoO109I 59 GGGCCCT [GA]G^G[ACGT]CC[CT] 77 GGGACCC EcoRII 63 CCTGG ^CC[AT]GG 315 CCTGG MaeII 97 ACGT A^CGT 161 ACGT MboI 463 GATC ^GATC 485 GATC MspA1I 24 CAGCGG C[AC]G^C[GT]G 150 CCGCTG MvaI 63 CCTGG CC^[AT]GG 315 CCTGG MvnI 54 CGCG CG^CG 56 CGCG NciI 81 CCCGG CC^[GC]GG 225 CCGGG NdeII 463 GATC ^GATC 485 GATC Sau3AI 463 GATC ^GATC 485 GATC SmlI 121 CTTGAG C^T[CT][GA]AG 394 CTTGAG TaqI 104 TCGA T^CGA 685 TCGA TfiI 537 GAATC G^A[AT]TC 678 GAATC TseI 406 GCAGC G^C[AT]GC 545 GCAGC TspRI 327 CAGTG CA[GC]TG[ACGT][ACGT]^ 552 CAGTG TthHB8I 104 TCGA T^CGA 685 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI AvaII BmyI Bsp1286I BssKI Cac8I CfoI Cfr13I DdeI FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboII MnlI MseI MspI MwoI NlaIV Sau96I ScrFI Tru9I Tsp45I