(Circular) (Six-base) MAPSORT of: pFN21AE4025.seq Check: 1317 from: 1 to: 5401 pFN21AE4025 5401 bp With 182 enzymes: SgfI * March 28, 2012 13:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3211 278 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 1937 Size: 807 4594 Acc65I G'GTAC_C Cuts at: 2722 2722 Size: 5401 AclI AA'CG_TT Cuts at: 1563 3528 3901 1563 Size: 1965 373 3063 Fragments arranged by size: 3063 1965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 2539 3464 4848 5116 1632 Size: 597 310 925 1384 268 1917 Fragments arranged by size: 1917 1384 925 597 310 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5204 AflIII A'CryG_T Cuts at: 1203 4300 5277 1203 Size: 3097 977 1327 Fragments arranged by size: 3097 1327 977 AgeI A'CCGG_T Cuts at: 2661 4232 2661 Size: 1571 3830 AhdI GACnn_n'nnGTC Cuts at: 4130 4130 Size: 5401 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5401 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5401 AlwNI CAG_nnn'CTG Cuts at: 2264 3144 4716 2264 Size: 880 1572 2949 Fragments arranged by size: 2949 1572 880 ApaI G_GGCC'C Cuts at: 2068 2068 Size: 5401 ApaLI G'TGCA_C Cuts at: 3458 4614 3458 Size: 1156 4245 ApoI r'AATT_y Cuts at: 1493 2710 2936 3088 5197 1493 Size: 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 3955 160 Size: 872 2923 1606 Fragments arranged by size: 2923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 AvaI C'yCGr_G Cuts at: 1870 1943 2726 1870 Size: 73 783 4545 Fragments arranged by size: 4545 783 73 BamHI G'GATC_C Cuts at: 2731 3098 2731 Size: 367 5034 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2722 4177 618 Size: 325 217 627 254 681 1455 1842 Fragments arranged by size: 1842 1455 681 627 325 254 217 BanII G_rGCy'C Cuts at: 1391 2012 2068 2720 1391 Size: 621 56 652 4072 Fragments arranged by size: 4072 652 621 56 BbeI G_GCGC'C Cuts at: 2045 2045 Size: 5401 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2460 928 Size: 971 561 3869 Fragments arranged by size: 3869 971 561 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3592 3626 1903 Size: 34 1655 34 3678 Fragments arranged by size: 3678 1655 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4503 3293 Size: 1210 4191 BclI T'GATC_A Cuts at: 1548 1548 Size: 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4012 136 Size: 107 122 71 631 605 2340 1525 Fragments arranged by size: 2340 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5396 5396 Size: 5401 BlpI GC'TnA_GC Cuts at: 2018 2818 2018 Size: 800 4601 Bme1580I G_kGCm'C Cuts at: 1790 2068 2347 3462 4618 1790 Size: 278 279 1115 1156 2573 Fragments arranged by size: 2573 1156 1115 279 278 BmgBI CAC'GTC Cuts at: 1318 2103 1318 Size: 785 4616 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 4090 442 Size: 416 53 83 399 24 1715 958 1753 Fragments arranged by size: 1753 1715 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 4061 1239 Size: 851 1971 2579 Fragments arranged by size: 2579 1971 851 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 2420 3526 4391 4689 2147 Size: 273 1106 865 298 2859 Fragments arranged by size: 2859 1106 865 298 273 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4064 882 Size: 617 2565 2219 Fragments arranged by size: 2565 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2167 3192 493 Size: 711 963 1025 2702 Fragments arranged by size: 2702 1025 963 711 BsaBI GATnn'nnATC Cuts at: 2541 5395 2541 Size: 2854 2547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 3208 3590 4225 Size: 53 83 186 936 509 1166 382 635 Cuts at: 4225 275 Size: 1451 Fragments arranged by size: 1451 1166 936 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2148 2661 3834 4232 4506 4653 5064 2148 Size: 513 1173 398 274 147 411 2485 Fragments arranged by size: 2485 1173 513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5176 Fragments arranged by size: 5176 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 1186 Size: 1231 4170 BseYI C'CCAG_C Cuts at: 4604 5100 4604 Size: 496 4905 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4828 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2851 3612 4640 1540 Size: 275 186 850 761 1028 2301 Fragments arranged by size: 2301 1028 850 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 3462 3547 4618 2024 Size: 1438 85 1071 2807 Fragments arranged by size: 2807 1438 1071 85 BsmI GAATG_Cn' Cuts at: 2912 3005 2912 Size: 93 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2024 2068 2347 2720 3462 3547 Size: 399 222 12 44 279 373 742 85 Cuts at: 3547 4618 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 279 222 85 44 12 BspEI T'CCGG_A Cuts at: 2148 5064 2148 Size: 2916 2485 BspHI T'CATG_A Cuts at: 3290 5020 3290 Size: 1730 3671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2756 844 Size: 1912 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3288 1394 Size: 429 1465 3507 Fragments arranged by size: 3507 1465 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3896 4070 65 Size: 1183 2648 174 1396 Fragments arranged by size: 2648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2661 4045 4232 1700 Size: 110 851 1384 187 2869 Fragments arranged by size: 2869 1384 851 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5401 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5401 BssSI C'ACGA_G Cuts at: 3461 4473 3461 Size: 1012 4389 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5401 BstBI TT'CG_AA Cuts at: 4205 4205 Size: 5401 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5401 BstYI r'GATC_y Cuts at: 1972 2731 3098 3483 3500 4952 5038 5050 5396 Size: 759 367 385 17 1452 86 12 346 Cuts at: 5396 1972 Size: 1977 Fragments arranged by size: 1977 1452 759 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3910 Fragments arranged by size: 3910 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4662 Fragments arranged by size: 4662 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3711 3731 2917 Size: 794 20 4587 Fragments arranged by size: 4587 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5366 DraI TTT'AAA Cuts at: 2705 2705 Size: 5401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4408 809 Size: 507 3092 1802 Fragments arranged by size: 3092 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2848 3737 Size: 54 1425 50 275 18 331 687 889 Cuts at: 3737 5366 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 687 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2848 1537 Size: 275 1036 4090 Fragments arranged by size: 4090 1036 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2644 3331 1353 Size: 1291 687 3423 Fragments arranged by size: 3423 1291 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3988 4362 4508 387 Size: 1064 138 2399 374 146 1280 Fragments arranged by size: 2399 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2229 2539 3464 4061 4848 5116 Size: 393 458 139 310 925 597 787 268 Cuts at: 5116 1239 Size: 1524 Fragments arranged by size: 1524 925 787 597 458 393 310 268 139 EcoNI CCTnn'n_nnAGG Cuts at: 2247 2247 Size: 5401 EcoO109I rG'GnC_Cy Cuts at: 2065 2083 2065 Size: 18 5383 EcoRI G'AATT_C Cuts at: 2710 3088 2710 Size: 378 5023 FspI TGC'GCA Cuts at: 3907 3907 Size: 5401 HaeII r_GCGC'y Cuts at: 1678 2045 4548 1678 Size: 367 2503 2531 Fragments arranged by size: 2531 2503 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373 Size: 32 135 32 2470 32 42 32 2626 Fragments arranged by size: 2626 2470 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2991 669 Size: 988 281 807 246 3079 Fragments arranged by size: 3079 988 807 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5401 HpaI GTT'AAC Cuts at: 2991 2991 Size: 5401 KasI G'GCGC_C Cuts at: 2041 2041 Size: 5401 KpnI G_GTAC'C Cuts at: 2726 2726 Size: 5401 MfeI C'AATT_G Cuts at: 3000 3000 Size: 5401 MluI A'CGCG_T Cuts at: 5277 5277 Size: 5401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4515 4699 1305 Size: 3210 184 2007 Fragments arranged by size: 3210 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2163 10 Size: 54 1425 343 331 3248 Fragments arranged by size: 3248 1425 343 331 54 MslI CAynn'nnrTG Cuts at: 518 1166 3359 3718 3877 518 Size: 648 2193 359 159 2042 Fragments arranged by size: 2193 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2157 2817 3494 4642 4887 1606 Size: 425 126 660 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 425 245 126 NarI GG'CG_CC Cuts at: 2042 2042 Size: 5401 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3910 Fragments arranged by size: 3910 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5401 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5401 NotI GC'GGCC_GC Cuts at: 2848 2848 Size: 5401 NspI r_CATG'y Cuts at: 1132 1229 2759 4304 1132 Size: 97 1530 1545 2229 Fragments arranged by size: 2229 1545 1530 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5401 PciI A'CATG_T Cuts at: 4300 4300 Size: 5401 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5401 PmeI GTTT'AAAC Cuts at: 2705 2705 Size: 5401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 4983 5015 3470 Size: 32 1481 32 3856 Fragments arranged by size: 3856 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 5401 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5401 PsiI TTA'TAA Cuts at: 2971 2971 Size: 5401 PspOMI G'GGCC_C Cuts at: 2064 2064 Size: 5401 PstI C_TGCA'G Cuts at: 830 2753 830 Size: 1923 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5401 SalI G'TCGA_C Cuts at: 1936 2743 1936 Size: 807 4594 SanDI GG'GwC_CC Cuts at: 2083 2083 Size: 5401 SbfI CC_TGCA'GG Cuts at: 2753 2753 Size: 5401 ScaI AGT'ACT Cuts at: 3649 5361 3649 Size: 1712 3689 SfcI C'TryA_G Cuts at: 826 1045 2749 3884 4565 4756 826 Size: 219 1704 1135 681 191 1471 Fragments arranged by size: 1704 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2043 2043 Size: 5401 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5401 SmaI CCC'GGG Cuts at: 1872 2728 1872 Size: 856 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2399 3505 4406 4668 820 Size: 197 926 183 273 1106 901 262 1553 Fragments arranged by size: 1553 1106 926 901 273 262 197 183 SnaBI TAC'GTA Cuts at: 493 3192 493 Size: 2699 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5401 SphI G_CATG'C Cuts at: 1132 2759 1132 Size: 1627 3774 SspI AAT'ATT Cuts at: 5 52 3325 5 Size: 47 3273 2081 Fragments arranged by size: 3273 2081 47 StuI AGG'CCT Cuts at: 1517 2503 1517 Size: 986 4415 StyI C'CwwG_G Cuts at: 513 1065 2004 2840 513 Size: 552 939 836 3074 Fragments arranged by size: 3074 939 836 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3428 3445 3598 3783 4228 325 Size: 1255 1848 17 153 185 445 1498 Fragments arranged by size: 1848 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 3647 5359 Size: 274 80 33 51 1443 390 1280 1712 Cuts at: 5359 96 Size: 138 Fragments arranged by size: 1712 1443 1280 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 5361 5393 2143 Size: 32 3186 32 2151 Fragments arranged by size: 3186 2151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5401 XbaI T'CTAG_A Cuts at: 2737 2737 Size: 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5401 XmaI C'CCGG_G Cuts at: 1870 2726 1870 Size: 856 4545 XmnI GAAnn'nnTTC Cuts at: 2253 3530 2253 Size: 1277 4124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3209 276 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI