################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MseI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tru9I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 673 GTAGAC GT^[AC][GT]AC AccIII 746 TCCGGA T^CCGGA AcsI 264 GAATTT [GA]^AATT[CT] AcyI 723 GACGCC G[GA]^CG[CT]C AflIII 116 ACATGT A^C[GA][CT]GT AluI 347 AGCT AG^CT ApoI 264 GAATTT [GA]^AATT[CT] Asp700I 496 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 619 CTCGAG C^[CT]CG[GA]G AvaII 615 GGTCC G^G[AT]CC BalI 134 TGGCCA TGG^CCA BbsI 170 GAAGAC GAAGAC(2/6) BclI 14 TGATCA T^GATCA BfaI 20 CTAG C^TAG BlpI 468 GCTAAGC GC^T[ACGT]AGC BpmI 62 CTCCAG CTGGAG(16/14) Bpu1102I 468 GCTAAGC GC^T[ACGT]AGC BpuAI 170 GAAGAC GAAGAC(2/6) BsaHI 723 GACGCC G[GA]^CG[CT]C BsaWI 746 TCCGGA [AT]^CCGG[AT] BseAI 746 TCCGGA T^CCGGA BsmAI 209 GTCTC GTCTC(1/5) BsmFI 681 GTCCC GGGAC(10/14) BsoBI 619 CTCGAG C^[CT]CG[GA]G Bsp1407I 119 TGTACA T^GTACA BspEI 746 TCCGGA T^CCGGA BspLU11I 116 ACATGT A^CATGT BsrGI 119 TGTACA T^GTACA BstYI 22 AGATCC [GA]^GATC[CT] Cac8I 541 GCAGGC GC[ACGT]^[ACGT]GC CelII 468 GCTAAGC GC^T[ACGT]AGC EarI 328 GAAGAG CTCTTC(1/4) EcoT14I 48 CCAAGG C^C[AT][AT]GG FauI 708 GCGGG CCCGC(4/6) FbaI 14 TGATCA T^GATCA HapII 747 CCGG C^CGG Hin1I 723 GACGCC G[GA]^CG[CT]C HpaII 747 CCGG C^CGG Ksp632I 328 GAAGAG CTCTTC(1/4) MaeI 20 CTAG C^TAG MflI 22 AGATCC [GA]^GATC[CT] MluNI 134 TGGCCA TGG^CCA MroI 746 TCCGGA T^CCGGA MscI 134 TGGCCA TGG^CCA MspI 747 CCGG C^CGG NspI 116 ACATGT [GA]CATG^[CT] PaeR7I 619 CTCGAG C^TCGAG PflMI 580 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 614 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 561 GACGCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmlI 619 CTCGAG C^T[CT][GA]AG SspBI 119 TGTACA T^GTACA SspI 338 AATATT AAT^ATT StyI 48 CCAAGG C^C[AT][AT]GG Tsp509I 265 AATT ^AATT TspRI 38 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 580 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 619 CTCGAG C^TCGAG XhoII 22 AGATCC [GA]^GATC[CT] XmnI 496 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 23 GATCC GGATC(4/5) 461 GGATC BanII 190 GAGCCC G[GA]GC[CT]^C 654 GGGCTC BbvI 128 GCAGC GCAGC(8/12) 630 GCAGC BmyI 190 GAGCCC G[AGT]GC[ACT]^C 654 GGGCTC BsaJI 48 CCAAGG C^C[ACGT][ACGT]GG 450 CCTCGG BseRI 286 CTCCTC GAGGAG(10/8) 668 GAGGAG Bsp1286I 190 GAGCCC G[AGT]GC[ACT]^C 654 GGGCTC BsrI 397 ACTGG ACTGG(1/-1) 684 CCAGT BssKI 132 CCTGG ^CC[ACGT]GG 612 CCAGG BstNI 132 CCTGG CC^[AT]GG 612 CCAGG DraII 412 AGGCCCT [GA]G^G[ACGT]CC[CT] 614 AGGTCCT EaeI 111 CGGCCA [CT]^GGCC[GA] 134 TGGCCA EcoO109I 412 AGGCCCT [GA]G^G[ACGT]CC[CT] 614 AGGTCCT EcoRII 132 CCTGG ^CC[AT]GG 612 CCAGG HgaI 561 GACGC GACGC(5/10) 723 GACGC MslI 97 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 99 CATTGATGTG MvaI 132 CCTGG CC^[AT]GG 612 CCAGG NlaIII 117 CATG CATG^ 357 CATG NlaIV 441 GGCCCC GG[ACGT]^[ACGT]CC 655 GGCTCC PleI 556 GACTC GAGTC(4/5) 676 GACTC ScrFI 132 CCTGG CC^[ACGT]GG 612 CCAGG SfaNI 31 GCATC GCATC(5/9) 53 GCATC TseI 128 GCAGC G^C[AT]GC 630 GCAGC Tsp45I 476 GTGAC ^GT[GC]AC 596 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AfaI BsiYI BslI BstF5I BstXI Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI NdeII RsaI Sau3AI Sau96I TaqI TfiI TthHB8I