(Circular) (Six-base) MAPSORT of: pFN21AE2307.seq Check: 888 from: 1 to: 5611 pFN21AE2307 5611 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2431 2954 1937 Size: 494 523 4594 Fragments arranged by size: 4594 523 494 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2762 3674 5058 5326 1632 Size: 1130 912 1384 268 1917 Fragments arranged by size: 1917 1384 1130 912 268 AfeI AGC'GCT Cuts at: 2831 2831 Size: 5611 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 2614 4510 5487 1203 Size: 1411 1896 977 1327 Fragments arranged by size: 1896 1411 1327 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 2591 3354 4926 2591 Size: 763 1572 3276 Fragments arranged by size: 3276 1572 763 ApaLI G'TGCA_C Cuts at: 3668 4824 3668 Size: 1156 4455 ApoI r'AATT_y Cuts at: 1493 2285 2920 3146 3298 5407 1493 Size: 792 635 226 152 2109 1697 Fragments arranged by size: 2109 1697 792 635 226 152 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2936 1870 Size: 73 993 4545 Fragments arranged by size: 4545 993 73 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2367 2932 4387 618 Size: 325 217 627 580 565 1455 1842 Fragments arranged by size: 1842 1455 627 580 565 325 217 BanII G_rGCy'C Cuts at: 1391 2692 2930 1391 Size: 1301 238 4072 Fragments arranged by size: 4072 1301 238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4640 BbvCI CC'TCA_GC Cuts at: 2731 2731 Size: 5611 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3802 3836 1903 Size: 34 50 34 1781 34 3678 Fragments arranged by size: 3678 1781 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2823 3503 4713 2823 Size: 680 1210 3721 Fragments arranged by size: 3721 1210 680 BclI T'GATC_A Cuts at: 1548 2353 1548 Size: 805 4806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4222 136 Size: 107 122 71 631 605 2550 1525 Fragments arranged by size: 2550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2447 5606 2447 Size: 3159 2452 BlpI GC'TnA_GC Cuts at: 3028 3028 Size: 5611 Bme1580I G_kGCm'C Cuts at: 1790 3672 4828 1790 Size: 1882 1156 2573 Fragments arranged by size: 2573 1882 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 3342 4300 Size: 416 53 83 399 24 874 1051 958 Cuts at: 4300 442 Size: 1753 Fragments arranged by size: 1753 1051 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4271 1239 Size: 3032 2579 Bpu10I CC'TnA_GC Cuts at: 2731 2731 Size: 5611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2512 3736 4601 4899 2512 Size: 1224 865 298 3224 Fragments arranged by size: 3224 1224 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4274 882 Size: 617 2775 2219 Fragments arranged by size: 2775 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3418 3800 4435 275 Size: 53 83 186 936 1885 382 635 1451 Fragments arranged by size: 1885 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2494 2524 1178 Size: 30 165 30 1091 30 4265 Fragments arranged by size: 4265 1091 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2515 1186 Size: 1329 4282 BseYI C'CCAG_C Cuts at: 2685 4814 5310 2685 Size: 2129 496 2986 Fragments arranged by size: 2986 2129 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5038 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3822 4850 1540 Size: 275 186 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3672 3757 4828 3672 Size: 85 1071 4455 Fragments arranged by size: 4455 1071 85 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2692 2930 3672 3757 4828 1391 Size: 399 902 238 742 85 1071 2174 Fragments arranged by size: 2174 1071 902 742 399 238 85 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2966 844 Size: 2122 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4255 4442 1700 Size: 110 2445 187 2869 Fragments arranged by size: 2869 2445 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5611 BssSI C'ACGA_G Cuts at: 2046 3671 4683 2046 Size: 1625 1012 2974 Fragments arranged by size: 2974 1625 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5611 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstXI CCAn_nnnn'nTGG Cuts at: 2653 2653 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2447 2941 3308 3693 3710 5162 5248 5260 Size: 475 494 367 385 17 1452 86 12 Cuts at: 5260 5606 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 494 475 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5059 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4872 Fragments arranged by size: 4872 683 56 BtsI GCAGTG_nn' Cuts at: 3127 3921 3941 3127 Size: 794 20 4797 Fragments arranged by size: 4797 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2392 2427 529 Size: 35 1828 35 3713 Fragments arranged by size: 3713 1828 35 35 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2655 3058 Size: 54 1425 50 275 18 345 480 403 Cuts at: 3058 3947 5576 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 480 403 345 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2404 2604 3541 1353 Size: 1051 200 937 3423 Fragments arranged by size: 3423 1051 937 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4198 4572 4718 387 Size: 1064 138 2609 374 146 1280 Fragments arranged by size: 2609 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2762 3674 4271 5058 5326 1239 Size: 393 1130 912 597 787 268 1524 Fragments arranged by size: 1524 1130 912 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2741 2741 Size: 5611 EcoRI G'AATT_C Cuts at: 2285 2920 3298 2285 Size: 635 378 4598 Fragments arranged by size: 4598 635 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 5611 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 2833 4758 1678 Size: 1155 1925 2531 Fragments arranged by size: 2531 1925 1155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373 Size: 32 135 32 2680 32 42 32 2626 Fragments arranged by size: 2680 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2955 3201 669 Size: 988 281 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 2553 2709 3210 2553 Size: 156 501 4954 Fragments arranged by size: 4954 501 156 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2427 4725 4909 1305 Size: 1122 2298 184 2007 Fragments arranged by size: 2298 2007 1122 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 2657 10 Size: 54 1425 343 345 480 2964 Fragments arranged by size: 2964 1425 480 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3569 3928 4087 518 Size: 648 2403 359 159 2042 Fragments arranged by size: 2403 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3027 3704 4852 5097 1606 Size: 1421 677 1148 245 2120 Fragments arranged by size: 2120 1421 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5059 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2618 2969 4514 1132 Size: 97 1389 351 1545 2229 Fragments arranged by size: 2229 1545 1389 351 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 2614 4510 2614 Size: 1896 3715 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5611 PfoI T'CCnGG_A Cuts at: 2380 2380 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2741 2741 Size: 5611 PshAI GACnn'nnGTC Cuts at: 1613 2393 1613 Size: 780 4831 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PstI C_TGCA'G Cuts at: 830 2963 830 Size: 2133 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SapI GCTCTTCn'nnn_ Cuts at: 2604 2604 Size: 5611 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SfcI C'TryA_G Cuts at: 826 1045 2592 2959 4094 4775 4966 826 Size: 219 1547 367 1135 681 191 1471 Fragments arranged by size: 1547 1471 1135 681 367 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5611 SmaI CCC'GGG Cuts at: 1872 2938 1872 Size: 1066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2527 3715 4616 4878 820 Size: 197 926 584 1188 901 262 1553 Fragments arranged by size: 1553 1188 926 901 584 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 2329 2856 3535 5 Size: 47 2277 527 679 2081 Fragments arranged by size: 2277 2081 679 527 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5611 StyI C'CwwG_G Cuts at: 513 1065 3050 513 Size: 552 1985 3074 Fragments arranged by size: 3074 1985 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2886 3638 3655 3808 3993 4438 325 Size: 1255 1306 752 17 153 185 445 1498 Fragments arranged by size: 1498 1306 1255 752 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3857 5569 96 Size: 274 80 33 51 1443 1880 1712 138 Fragments arranged by size: 1880 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5611 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2378 2378 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2936 1870 Size: 1066 4545 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI