(Circular) (Six-base) MAPSORT of: pFN21AE3216.seq Check: 795 from: 1 to: 6184 pFN21AE3216 6184 bp With 182 enzymes: SgfI * March 28, 2012 12:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3196 3505 3196 Size: 309 5875 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2670 2934 3501 4247 5631 5899 1632 Size: 1038 264 567 746 1384 268 1917 Fragments arranged by size: 1917 1384 1038 746 567 268 264 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3368 1184 Size: 2184 4000 AlwNI CAG_nnn'CTG Cuts at: 2396 2645 3927 5499 2396 Size: 249 1282 1572 3081 Fragments arranged by size: 3081 1572 1282 249 ApaLI G'TGCA_C Cuts at: 3380 4241 5397 3380 Size: 861 1156 4167 Fragments arranged by size: 4167 1156 861 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2057 3509 1870 Size: 73 114 1452 4545 Fragments arranged by size: 4545 1452 114 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3381 3414 3381 Size: 33 6151 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2629 3196 3505 4960 618 Size: 325 217 627 842 567 309 1455 1842 Fragments arranged by size: 1842 1455 842 627 567 325 309 217 BanII G_rGCy'C Cuts at: 1391 3503 1391 Size: 2112 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 2569 2824 2968 1548 Size: 1021 255 144 4764 Fragments arranged by size: 4764 1021 255 144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2173 6179 2173 Size: 4006 2178 BlpI GC'TnA_GC Cuts at: 2523 3601 2523 Size: 1078 5106 Bme1580I G_kGCm'C Cuts at: 1790 3384 4245 5401 1790 Size: 1594 861 1156 2573 Fragments arranged by size: 2573 1594 1156 861 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2359 3306 3915 Size: 416 53 83 399 24 942 947 609 Cuts at: 3915 4873 442 Size: 958 1753 Fragments arranged by size: 1753 958 947 942 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2096 2096 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3414 4844 1239 Size: 2175 1430 2579 Fragments arranged by size: 2579 2175 1430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 4309 5174 5472 2030 Size: 2279 865 298 2742 Fragments arranged by size: 2742 2279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5959 Fragments arranged by size: 5959 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2661 3125 3320 1186 Size: 1475 464 195 4050 Fragments arranged by size: 4050 1475 464 195 BseYI C'CCAG_C Cuts at: 2509 2518 5387 5883 2509 Size: 9 2869 496 2810 Fragments arranged by size: 2869 2810 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2723 3186 3239 3429 1163 Size: 573 987 463 53 190 3918 Fragments arranged by size: 3918 987 573 463 190 53 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3357 3384 4245 4330 5401 3357 Size: 27 861 85 1071 4140 Fragments arranged by size: 4140 1071 861 85 27 BsmI GAATG_Cn' Cuts at: 3121 3695 3788 3121 Size: 574 93 5517 Fragments arranged by size: 5517 574 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3357 3384 3503 4245 4330 5401 1391 Size: 399 1567 27 119 742 85 1071 2174 Fragments arranged by size: 2174 1567 1071 742 399 119 85 27 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 4073 5803 4073 Size: 1730 4454 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2695 3208 3539 844 Size: 1851 513 331 3489 Fragments arranged by size: 3489 1851 513 331 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4679 4853 65 Size: 1183 3431 174 1396 Fragments arranged by size: 3431 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 3024 4244 5256 3024 Size: 1220 1012 3952 Fragments arranged by size: 3952 1220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3224 794 Size: 2430 3754 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstYI r'GATC_y Cuts at: 1972 2173 3514 3881 4266 4283 5735 5821 5833 Size: 201 1341 367 385 17 1452 86 12 Cuts at: 5833 6179 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1341 385 367 346 201 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2835 3192 2835 Size: 357 5827 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5632 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 3384 3700 4494 4514 3384 Size: 316 794 20 5054 Fragments arranged by size: 5054 794 316 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 2253 3488 2253 Size: 1235 4949 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3631 4520 6149 Size: 54 1425 50 275 18 1801 889 1629 Cuts at: 6149 8 Size: 43 Fragments arranged by size: 1801 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4114 1353 Size: 2761 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2670 2934 3414 3501 4247 4844 5631 Size: 393 1038 264 480 87 746 597 787 Cuts at: 5631 5899 1239 Size: 268 1524 Fragments arranged by size: 1524 1038 787 746 597 480 393 268 264 87 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 5331 1678 Size: 3653 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2852 2884 4825 Size: 32 135 32 626 32 622 32 1941 Cuts at: 4825 4857 4899 4931 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1941 626 622 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3528 3774 669 Size: 988 281 933 657 246 3079 Fragments arranged by size: 3079 988 933 657 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3200 3509 3200 Size: 309 5875 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2501 3087 5298 5482 1305 Size: 1196 586 2211 184 2007 Fragments arranged by size: 2211 2007 1196 586 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4362 Fragments arranged by size: 4362 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4142 4501 4660 518 Size: 648 2976 359 159 2042 Fragments arranged by size: 2976 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2483 2513 3479 3600 4277 5425 5670 1606 Size: 877 30 966 121 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 877 677 245 121 30 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5632 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3070 3542 5087 1132 Size: 97 1841 472 1545 2229 Fragments arranged by size: 2229 1841 1545 472 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 3337 1108 Size: 2229 3955 PfoI T'CCnGG_A Cuts at: 3210 3210 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspXI vC'TCGA_Gb Cuts at: 2057 2057 Size: 6184 PstI C_TGCA'G Cuts at: 830 3373 3536 830 Size: 2543 163 3478 Fragments arranged by size: 3478 2543 163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2483 2513 3479 2483 Size: 30 966 5188 Fragments arranged by size: 5188 966 30 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2274 2310 3042 3081 3369 3532 4667 Size: 219 1229 36 732 39 288 163 1135 Cuts at: 4667 5348 5539 826 Size: 681 191 1471 Fragments arranged by size: 1471 1229 1135 732 681 288 219 191 163 39 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2057 4288 5189 5451 820 Size: 197 926 102 12 2231 901 262 1553 Fragments arranged by size: 2231 1553 926 901 262 197 102 12 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 3125 4108 5 Size: 47 3073 983 2081 Fragments arranged by size: 3073 2081 983 47 StuI AGG'CCT Cuts at: 1517 3438 1517 Size: 1921 4263 StyI C'CwwG_G Cuts at: 513 1065 3623 513 Size: 552 2558 3074 Fragments arranged by size: 3074 2558 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2378 4211 4228 4381 4566 5011 325 Size: 1255 798 1833 17 153 185 445 1498 Fragments arranged by size: 1833 1498 1255 798 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2353 2385 6144 6176 2353 Size: 32 3759 32 2361 Fragments arranged by size: 3759 2361 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 2057 1943 Size: 114 6070 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 4313 4313 Size: 6184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI