(Linear) (Six-base) MAPSORT of: KIEE3216.seq Check: 1278 from: 1 to: 1479 KIEE3216.orf With 182 enzymes: SgfI * March 22, 2012 16:22 .. Acc65I G'GTAC_C Cuts at: 0 1191 1479 Size: 1191 288 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 929 1479 Size: 665 264 550 Fragments arranged by size: 665 550 264 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1363 1479 Size: 1363 116 AlwNI CAG_nnn'CTG Cuts at: 0 391 640 1479 Size: 391 249 839 Fragments arranged by size: 839 391 249 ApaLI G'TGCA_C Cuts at: 0 1375 1479 Size: 1375 104 AvaI C'yCGr_G Cuts at: 0 52 1479 Size: 52 1427 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1376 1409 1479 Size: 1376 33 70 Fragments arranged by size: 1376 70 33 BanI G'GyrC_C Cuts at: 0 624 1191 1479 Size: 624 567 288 Fragments arranged by size: 624 567 288 BclI T'GATC_A Cuts at: 0 564 819 963 1479 Size: 564 255 144 516 Fragments arranged by size: 564 516 255 144 BglII A'GATC_T Cuts at: 0 168 1479 Size: 168 1311 BlpI GC'TnA_GC Cuts at: 0 518 1479 Size: 518 961 Bme1580I G_kGCm'C Cuts at: 0 1379 1479 Size: 1379 100 BmrI ACTGGGnnnn_n' Cuts at: 0 354 1301 1479 Size: 354 947 178 Fragments arranged by size: 947 354 178 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 91 1479 Size: 91 1388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1409 1479 Size: 1409 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 1479 Size: 25 1454 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 656 1120 1315 1479 Size: 656 464 195 164 Fragments arranged by size: 656 464 195 164 BseYI C'CCAG_C Cuts at: 0 504 513 1479 Size: 504 9 966 Fragments arranged by size: 966 504 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1181 1234 1424 1479 Size: 718 463 53 190 55 Fragments arranged by size: 718 463 190 55 53 BsiHKAI G_wGCw'C Cuts at: 0 1352 1379 1479 Size: 1352 27 100 Fragments arranged by size: 1352 100 27 BsmI GAATG_Cn' Cuts at: 0 1116 1479 Size: 1116 363 Bsp1286I G_dGCh'C Cuts at: 0 1352 1379 1479 Size: 1352 27 100 Fragments arranged by size: 1352 100 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 690 1203 1479 Size: 690 513 276 Fragments arranged by size: 690 513 276 BssSI C'ACGA_G Cuts at: 0 1019 1479 Size: 1019 460 BstAPI GCAn_nnn'nTGC Cuts at: 0 1219 1479 Size: 1219 260 BstYI r'GATC_y Cuts at: 0 168 1479 Size: 168 1311 Bsu36I CC'TnA_GG Cuts at: 0 830 1187 1479 Size: 830 357 292 Fragments arranged by size: 830 357 292 BtsI GCAGTG_nn' Cuts at: 0 1379 1479 Size: 1379 100 DraI TTT'AAA Cuts at: 0 248 1479 Size: 248 1231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 929 1409 1479 Size: 665 264 480 70 Fragments arranged by size: 665 480 264 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 847 879 1479 Size: 193 32 622 32 600 Fragments arranged by size: 622 600 193 32 32 HincII GTy'rAC Cuts at: 0 866 1479 Size: 866 613 KpnI G_GTAC'C Cuts at: 0 1195 1479 Size: 1195 284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 496 1082 1479 Size: 496 586 397 Fragments arranged by size: 586 496 397 MspA1I CmG'CkG Cuts at: 0 478 508 1474 1479 Size: 478 30 966 5 Fragments arranged by size: 966 478 30 5 NspI r_CATG'y Cuts at: 0 1065 1479 Size: 1065 414 PflMI CCAn_nnn'nTGG Cuts at: 0 1332 1479 Size: 1332 147 PfoI T'CCnGG_A Cuts at: 0 1205 1479 Size: 1205 274 PspXI vC'TCGA_Gb Cuts at: 0 52 1479 Size: 52 1427 PstI C_TGCA'G Cuts at: 0 1368 1479 Size: 1368 111 PvuII CAG'CTG Cuts at: 0 478 508 1474 1479 Size: 478 30 966 5 Fragments arranged by size: 966 478 30 5 SfcI C'TryA_G Cuts at: 0 269 305 1037 1076 1364 1479 Size: 269 36 732 39 288 115 Fragments arranged by size: 732 288 269 115 39 36 SmlI C'TyrA_G Cuts at: 0 40 52 1479 Size: 40 12 1427 Fragments arranged by size: 1427 40 12 SspI AAT'ATT Cuts at: 0 1120 1479 Size: 1120 359 StuI AGG'CCT Cuts at: 0 1433 1479 Size: 1433 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 373 1479 Size: 373 1106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 348 380 1479 Size: 348 32 1099 Fragments arranged by size: 1099 348 32 XhoI C'TCGA_G Cuts at: 0 52 1479 Size: 52 1427 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApaLI AvaI BaeI BanI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BssSI BstAPI BstYI Bsu36I BtsI DraI Eco57MI Hin4I HincII KpnI MmeI MspA1I NspI PflMI PfoI PspXI PstI PvuII SfcI SmlI SspI StuI TaqII TstI XhoI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApoI AscI AseI AsiSI AvrII BamHI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI