(Circular) (Six-base) MAPSORT of: pFN21AE3226.seq Check: 9274 from: 1 to: 6262 pFN21AE3226 6262 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3605 1937 Size: 1668 4594 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2211 2409 2935 3080 4325 5709 5977 Size: 564 15 198 526 145 1245 1384 268 Cuts at: 5977 1632 Size: 1917 Fragments arranged by size: 1917 1384 1245 564 526 268 198 145 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 2714 5161 6138 1203 Size: 1511 2447 977 1327 Fragments arranged by size: 2447 1511 1327 977 AgeI A'CCGG_T Cuts at: 2443 5093 2443 Size: 2650 3612 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3305 3337 3305 Size: 32 6230 AlwNI CAG_nnn'CTG Cuts at: 4005 5577 4005 Size: 1572 4690 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2309 2545 2897 3033 3571 3797 3949 6058 Size: 816 236 352 136 538 226 152 2109 Cuts at: 6058 1493 Size: 1697 Fragments arranged by size: 2109 1697 816 538 352 236 226 152 136 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2036 3587 1870 Size: 73 93 1551 4545 Fragments arranged by size: 4545 1551 93 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3583 5038 618 Size: 325 217 627 1796 1455 1842 Fragments arranged by size: 1842 1796 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 3581 1391 Size: 646 1544 4072 Fragments arranged by size: 4072 1544 646 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2379 928 Size: 971 480 4811 Fragments arranged by size: 4811 971 480 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2396 2430 4453 4487 1903 Size: 34 459 34 2023 34 3678 Fragments arranged by size: 3678 2023 459 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2404 4154 5364 2404 Size: 1750 1210 3302 Fragments arranged by size: 3302 1750 1210 BclI T'GATC_A Cuts at: 1548 3097 1548 Size: 1549 4713 BfrBI ATG'CAT Cuts at: 2807 2807 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4873 136 Size: 107 122 71 631 605 3201 1525 Fragments arranged by size: 3201 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 2659 4323 5479 1790 Size: 869 1664 1156 2573 Fragments arranged by size: 2573 1664 1156 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3993 4951 442 Size: 416 53 83 399 24 2576 958 1753 Fragments arranged by size: 2576 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 3332 4922 1239 Size: 990 1103 1590 2579 Fragments arranged by size: 2579 1590 1103 990 Bpu10I CC'TnA_GC Cuts at: 2031 2291 2031 Size: 260 6002 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4387 5252 5550 4387 Size: 865 298 5099 Fragments arranged by size: 5099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3531 4925 882 Size: 617 2032 1394 2219 Fragments arranged by size: 2219 2032 1394 617 BsaAI yAC'GTr Cuts at: 493 1204 2969 4053 493 Size: 711 1765 1084 2702 Fragments arranged by size: 2702 1765 1084 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4069 4451 5086 275 Size: 53 83 186 936 2536 382 635 1451 Fragments arranged by size: 2536 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2443 4695 5093 5367 5514 5925 2443 Size: 2252 398 274 147 411 2780 Fragments arranged by size: 2780 2252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3305 3335 1178 Size: 30 165 30 1902 30 4105 Fragments arranged by size: 4105 1902 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3378 1186 Size: 2192 4070 BseYI C'CCAG_C Cuts at: 2060 5465 5961 2060 Size: 3405 496 2361 Fragments arranged by size: 3405 2361 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5689 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4323 4408 5479 4323 Size: 85 1071 5106 Fragments arranged by size: 5106 1071 85 BsmI GAATG_Cn' Cuts at: 2680 3024 3773 3866 2680 Size: 344 749 93 5076 Fragments arranged by size: 5076 749 344 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2659 3581 4323 4408 5479 1391 Size: 399 247 622 922 742 85 1071 2174 Fragments arranged by size: 2174 1071 922 742 622 399 247 85 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 3196 4151 5881 3196 Size: 955 1730 3577 Fragments arranged by size: 3577 1730 955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3617 844 Size: 2773 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4149 1394 Size: 429 2326 3507 Fragments arranged by size: 3507 2326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2443 4906 5093 1700 Size: 110 633 2463 187 2869 Fragments arranged by size: 2869 2463 633 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 2415 4322 5334 2415 Size: 1907 1012 3343 Fragments arranged by size: 3343 1907 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6262 BstYI r'GATC_y Cuts at: 1972 2008 3592 3959 4344 4361 5813 5899 5911 Size: 36 1584 367 385 17 1452 86 12 Cuts at: 5911 6257 1972 Size: 346 1977 Fragments arranged by size: 1977 1584 1452 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2072 513 Size: 552 939 68 4703 Fragments arranged by size: 4703 939 552 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2010 487 Size: 683 56 784 4739 Fragments arranged by size: 4739 784 683 56 BtsI GCAGTG_nn' Cuts at: 3778 4572 4592 3778 Size: 794 20 5448 Fragments arranged by size: 5448 794 20 ClaI AT'CG_AT Cuts at: 1554 3270 1554 Size: 1716 4546 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 2592 2622 2881 2941 3566 2592 Size: 30 259 60 625 5288 Fragments arranged by size: 5288 625 259 60 30 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3091 3709 Size: 54 1425 50 275 18 303 958 618 Cuts at: 3709 4598 6227 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 958 889 618 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3177 3401 4192 1353 Size: 1824 224 791 3423 Fragments arranged by size: 3423 1824 791 224 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4849 5223 5369 387 Size: 1064 138 3260 374 146 1280 Fragments arranged by size: 3260 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2211 2229 2409 2935 3080 3332 Size: 393 564 15 18 180 526 145 252 Cuts at: 3332 4325 4922 5709 5977 1239 Size: 993 597 787 268 1524 Fragments arranged by size: 1524 993 787 597 564 526 393 268 252 180 145 18 15 EcoO109I rG'GnC_Cy Cuts at: 2057 2057 Size: 6262 EcoRI G'AATT_C Cuts at: 3033 3571 3949 3033 Size: 538 378 5346 Fragments arranged by size: 5346 538 378 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 6262 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 5409 1678 Size: 3731 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4903 4935 4977 5009 1373 Size: 32 135 32 3331 32 42 32 2626 Fragments arranged by size: 3331 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2401 2740 3606 3852 669 Size: 988 281 463 339 866 246 3079 Fragments arranged by size: 3079 988 866 463 339 281 246 HindIII A'AGCT_T Cuts at: 748 3462 748 Size: 2714 3548 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2733 3068 5376 5560 1305 Size: 1428 335 2308 184 2007 Fragments arranged by size: 2308 2007 1428 335 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 3093 10 Size: 54 1425 343 303 958 3179 Fragments arranged by size: 3179 1425 958 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2808 2981 4220 4579 4738 518 Size: 648 1201 441 173 1239 359 159 2042 Fragments arranged by size: 2042 1239 1201 648 441 359 173 159 MspA1I CmG'CkG Cuts at: 1606 2048 2131 3678 4355 5503 5748 1606 Size: 442 83 1547 677 1148 245 2120 Fragments arranged by size: 2120 1547 1148 677 442 245 83 NcoI C'CATG_G Cuts at: 513 1065 2004 2072 513 Size: 552 939 68 4703 Fragments arranged by size: 4703 939 552 68 NdeI CA'TA_TG Cuts at: 387 2809 387 Size: 2422 3840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NsiI A_TGCA'T Cuts at: 2809 2809 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 2633 2986 3241 3251 3620 5165 1132 Size: 97 1404 353 255 10 369 1545 2229 Fragments arranged by size: 2229 1545 1404 369 353 255 97 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PfoI T'CCnGG_A Cuts at: 3324 3324 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3305 3337 3378 3410 4331 4363 5844 5876 3305 Size: 32 41 32 921 32 1481 32 3691 Fragments arranged by size: 3691 1481 921 41 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2057 Size: 6262 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6262 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PstI C_TGCA'G Cuts at: 830 3335 3614 830 Size: 2505 279 3478 Fragments arranged by size: 3478 2505 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 2131 2131 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SanDI GG'GwC_CC Cuts at: 2057 2057 Size: 6262 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SfcI C'TryA_G Cuts at: 826 1045 2255 2428 2685 2856 3331 3610 4745 Size: 219 1210 173 257 171 475 279 1135 Cuts at: 4745 5426 5617 826 Size: 681 191 1471 Fragments arranged by size: 1471 1210 1135 681 475 279 257 219 191 173 171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 2038 3589 1872 Size: 166 1551 4545 Fragments arranged by size: 4545 1551 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 4366 5267 5529 820 Size: 197 926 2423 901 262 1553 Fragments arranged by size: 2423 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3251 3620 1132 Size: 2119 369 3774 Fragments arranged by size: 3774 2119 369 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2004 2072 3701 513 Size: 552 939 68 1629 3074 Fragments arranged by size: 3074 1629 939 552 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4289 4306 4459 4644 5089 325 Size: 1255 2709 17 153 185 445 1498 Fragments arranged by size: 2709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2777 3052 4508 Size: 274 80 33 51 1443 800 275 1456 Cuts at: 4508 6220 96 Size: 1712 138 Fragments arranged by size: 1712 1456 1443 800 275 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6222 6254 6222 Size: 32 6230 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6262 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 2036 3587 1870 Size: 166 1551 4545 Fragments arranged by size: 4545 1551 166 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI