Restriction map for
pFN21AE3226
(Linear) (Six-base) MAPSORT of: KIEE3226.seq Check: 8216 from: 1 to: 1557
KIEE3226.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 206 404 930 1075 1557
Size: 191 15 198 526 145 482
Fragments arranged by size:
526 482 198 191 145 15
AflIII A'CryG_T
Cuts at: 0 709 1557
Size: 709 848
AgeI A'CCGG_T
Cuts at: 0 438 1557
Size: 438 1119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 1557
Size: 1300 32 225
Fragments arranged by size:
1300 225 32
ApoI r'AATT_y
Cuts at: 0 304 540 892 1028 1557
Size: 304 236 352 136 529
Fragments arranged by size:
529 352 304 236 136
AvaI C'yCGr_G
Cuts at: 0 31 1557
Size: 31 1526
BanII G_rGCy'C
Cuts at: 0 32 1557
Size: 32 1525
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 1557
Size: 374 1183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 425 1557
Size: 391 34 1132
Fragments arranged by size:
1132 391 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 399 1557
Size: 399 1158
BclI T'GATC_A
Cuts at: 0 1092 1557
Size: 1092 465
BfrBI ATG'CAT
Cuts at: 0 802 1557
Size: 802 755
Bme1580I G_kGCm'C
Cuts at: 0 654 1557
Size: 654 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1327 1557
Size: 224 1103 230
Fragments arranged by size:
1103 230 224
Bpu10I CC'TnA_GC
Cuts at: 0 26 286 1557
Size: 26 260 1271
Fragments arranged by size:
1271 260 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 1526 1557
Size: 1526 31
BsaAI yAC'GTr
Cuts at: 0 964 1557
Size: 964 593
BsaWI w'CCGG_w
Cuts at: 0 438 1557
Size: 438 1119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1300 1330 1557
Size: 1300 30 227
Fragments arranged by size:
1300 227 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1373 1557
Size: 1373 184
BseYI C'CCAG_C
Cuts at: 0 55 1557
Size: 55 1502
BsmI GAATG_Cn'
Cuts at: 0 675 1019 1557
Size: 675 344 538
Fragments arranged by size:
675 538 344
Bsp1286I G_dGCh'C
Cuts at: 0 32 654 1557
Size: 32 622 903
Fragments arranged by size:
903 622 32
BspHI T'CATG_A
Cuts at: 0 1191 1557
Size: 1191 366
BsrFI r'CCGG_y
Cuts at: 0 438 1557
Size: 438 1119
BssSI C'ACGA_G
Cuts at: 0 410 1557
Size: 410 1147
BstYI r'GATC_y
Cuts at: 0 3 1557
Size: 3 1554
BtgI C'CryG_G
Cuts at: 0 67 1557
Size: 67 1490
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5 1557
Size: 5 1552
ClaI AT'CG_AT
Cuts at: 0 1265 1557
Size: 1265 292
DraI TTT'AAA
Cuts at: 0 587 617 876 936 1557
Size: 587 30 259 60 621
Fragments arranged by size:
621 587 259 60 30
EaeI y'GGCC_r
Cuts at: 0 128 1086 1557
Size: 128 958 471
Fragments arranged by size:
958 471 128
EarI CTCTTCn'nnn_
Cuts at: 0 1172 1396 1557
Size: 1172 224 161
Fragments arranged by size:
1172 224 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 206 224 404 930 1075 1327 1557
Size: 191 15 18 180 526 145 252 230
Fragments arranged by size:
526 252 230 191 180 145 18 15
EcoO109I rG'GnC_Cy
Cuts at: 0 52 1557
Size: 52 1505
EcoRI G'AATT_C
Cuts at: 0 1028 1557
Size: 1028 529
EcoRV GAT'ATC
Cuts at: 0 483 1557
Size: 483 1074
HincII GTy'rAC
Cuts at: 0 396 735 1557
Size: 396 339 822
Fragments arranged by size:
822 396 339
HindIII A'AGCT_T
Cuts at: 0 1457 1557
Size: 1457 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1063 1557
Size: 728 335 494
Fragments arranged by size:
728 494 335
MscI TGG'CCA
Cuts at: 0 130 1088 1557
Size: 130 958 469
Fragments arranged by size:
958 469 130
MslI CAynn'nnrTG
Cuts at: 0 362 803 976 1557
Size: 362 441 173 581
Fragments arranged by size:
581 441 362 173
MspA1I CmG'CkG
Cuts at: 0 43 126 1557
Size: 43 83 1431
Fragments arranged by size:
1431 83 43
NcoI C'CATG_G
Cuts at: 0 67 1557
Size: 67 1490
NdeI CA'TA_TG
Cuts at: 0 804 1557
Size: 804 753
NsiI A_TGCA'T
Cuts at: 0 804 1557
Size: 804 753
NspI r_CATG'y
Cuts at: 0 628 981 1236 1246 1557
Size: 628 353 255 10 311
Fragments arranged by size:
628 353 311 255 10
PfoI T'CCnGG_A
Cuts at: 0 1319 1557
Size: 1319 238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 1373 1405 1557
Size: 1300 32 41 32 152
Fragments arranged by size:
1300 152 41 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 52 1557
Size: 52 1505
PstI C_TGCA'G
Cuts at: 0 1330 1557
Size: 1330 227
PvuII CAG'CTG
Cuts at: 0 126 1557
Size: 126 1431
SanDI GG'GwC_CC
Cuts at: 0 52 1557
Size: 52 1505
SfcI C'TryA_G
Cuts at: 0 250 423 680 851 1326 1557
Size: 250 173 257 171 475 231
Fragments arranged by size:
475 257 250 231 173 171
SmaI CCC'GGG
Cuts at: 0 33 1557
Size: 33 1524
SphI G_CATG'C
Cuts at: 0 1246 1557
Size: 1246 311
StyI C'CwwG_G
Cuts at: 0 67 1557
Size: 67 1490
TatI w'GTAC_w
Cuts at: 0 772 1047 1557
Size: 772 275 510
Fragments arranged by size:
772 510 275
XmaI C'CCGG_G
Cuts at: 0 31 1557
Size: 31 1526
Enzymes that do cut:
AcuI AflIII AgeI AloI ApoI AvaI BanII BbsI
BcgI BciVI BclI BfrBI Bme1580I BpmI Bpu10I BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsmI Bsp1286I BspHI
BsrFI BssSI BstYI BtgI BtgZI ClaI DraI EaeI
EarI Eco57MI EcoO109I EcoRI EcoRV HincII HindIII MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PfoI
PpiI PpuMI PstI PvuII SanDI SfcI SmaI SphI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI BsaHI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI FalI FseI FspI FspAI HaeII Hin4I HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI