################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BclI BfaI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsmAI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I DraI DraII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI ScaI SfiI SfoI SfuI SnaBI SpeI SspBI SspI StyI SwaI TfiI TspRI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 111 CGCG CG^CG AcsI 827 AAATTT [GA]^AATT[CT] AflII 651 CTTAAG C^TTAAG Alw44I 616 GTGCAC G^TGCAC ApaLI 616 GTGCAC G^TGCAC ApoI 827 AAATTT [GA]^AATT[CT] AspHI 616 GTGCAC G[AT]GC[AT]^C AvaI 251 CCCGGG C^[CT]CG[GA]G BanI 405 GGTGCC G^G[CT][GA]CC BanII 792 GAGCCC G[GA]GC[CT]^C BciVI 204 GGATAC GTATCC(6/5) BfrI 651 CTTAAG C^TTAAG BlpI 567 GCTGAGC GC^T[ACGT]AGC Bpu1102I 567 GCTGAGC GC^T[ACGT]AGC BsiHKAI 616 GTGCAC G[AT]GC[AT]^C BsiWI 125 CGTACG C^GTACG BsmI 693 GAATGC GAATGC(1/-1) BsoBI 251 CCCGGG C^[CT]CG[GA]G BspMI 402 GCAGGT ACCTGC(4/8) BstUI 111 CGCG CG^CG CelII 567 GCTGAGC GC^T[ACGT]AGC CpoI 20 CGGTCCG CG^G[AT]CCG DdeI 568 CTGAG C^T[ACGT]AG DsaI 410 CCACGG C^C[GA][CT]GG MaeII 71 ACGT A^CGT MaeIII 448 GTGAC ^GT[ACGT]AC MboII 221 GAAGA GAAGA(8/7) MslI 609 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 111 CGCG CG^CG NspI 342 GCATGC [GA]CATG^[CT] PvuII 819 CAGCTG CAG^CTG RsrII 20 CGGTCCG CG^G[AT]CCG SbfI 99 CCTGCAGG CCTGCA^GG SexAI 620 ACCTGGT A^CC[AT]GGT SgrAI 611 CGCCGGTG C[GA]^CCGG[CT]G SmaI 251 CCCGGG CCC^GGG SphI 342 GCATGC GCATG^C Sse8387I 99 CCTGCAGG CCTGCA^GG StuI 425 AGGCCT AGG^CCT Tsp45I 448 GTGAC ^GT[GC]AC XmaCI 251 CCCGGG C^CCGGG XmaI 251 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 126 GTAC GT^AC 230 GTAC AvaII 21 GGTCC G^G[AT]CC 540 GGACC BalI 509 TGGCCA TGG^CCA 680 TGGCCA BcgI 336 CGATGAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 557 CGAGCAAGGTGC BsmFI 120 GTCCC GGGAC(10/14) 355 GTCCC BspDI 317 ATCGAT AT^CGAT 334 ATCGAT BsrBI 240 CCGCTC CCGCTC(-3/-3) 637 CCGCTC BsrDI 285 CATTGC GCAATG(2/0) 645 CATTGC BsrFI 542 ACCGGC [GA]^CCGG[CT] 612 GCCGGT BstXI 164 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 359 CCAAGATCCTGG BstYI 86 AGATCC [GA]^GATC[CT] 362 AGATCC Cfr10I 542 ACCGGC [GA]^CCGG[CT] 612 GCCGGT ClaI 317 ATCGAT AT^CGAT 334 ATCGAT DraIII 260 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 619 CACCTGGTG EagI 113 CGGCCG C^GGCCG 553 CGGCCG EclXI 113 CGGCCG C^GGCCG 553 CGGCCG Eco52I 113 CGGCCG C^GGCCG 553 CGGCCG HaeII 4 AGCGCC [GA]GCGC^[CT] 437 AGCGCC HphI 186 GGTGA GGTGA(8/7) 728 TCACC MflI 86 AGATCC [GA]^GATC[CT] 362 AGATCC MluNI 509 TGGCCA TGG^CCA 680 TGGCCA MscI 509 TGGCCA TGG^CCA 680 TGGCCA MseI 326 TTAA T^TAA 652 TTAA MspA1I 294 CCGCTG C[AC]G^C[GT]G 819 CAGCTG NlaIII 343 CATG CATG^ 532 CATG PstI 100 CTGCAG CTGCA^G 193 CTGCAG RsaI 126 GTAC GT^AC 230 GTAC SfaNI 705 GATGC GCATC(5/9) 143 GCATC SfcI 100 CTGCAG C^T[GA][CT]AG 193 CTGCAG Tru9I 326 TTAA T^TAA 652 TTAA Tsp509I 478 AATT ^AATT 828 AATT XcmI 161 CCACCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 359 CCAAGATCCTGGTGG XhoII 86 AGATCC [GA]^GATC[CT] 362 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BmyI BsaJI BsgI BsiEI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspI MvaI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI SmlI TaqI TseI TthHB8I