(Circular) (Six-base) MAPSORT of: pFN21AE3109.seq Check: 1829 from: 1 to: 5539 pFN21AE3109 5539 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2398 2398 Size: 5539 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2882 1937 Size: 945 4594 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 3666 4039 1563 Size: 2103 373 3063 Fragments arranged by size: 3063 2103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 3602 4986 5254 1632 Size: 567 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 567 268 AflII C'TTAA_G Cuts at: 820 1017 2656 820 Size: 197 1639 3703 Fragments arranged by size: 3703 1639 197 AflIII A'CryG_T Cuts at: 1203 4438 5415 1203 Size: 3235 977 1327 Fragments arranged by size: 3235 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 2618 1166 Size: 1452 4087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2636 1184 Size: 1452 4087 AlwNI CAG_nnn'CTG Cuts at: 3282 4854 3282 Size: 1572 3967 ApaLI G'TGCA_C Cuts at: 2621 3596 4752 2621 Size: 975 1156 3408 Fragments arranged by size: 3408 1156 975 ApoI r'AATT_y Cuts at: 1493 2832 2848 3074 3226 5335 1493 Size: 1339 16 226 152 2109 1697 Fragments arranged by size: 2109 1697 1339 226 152 16 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2256 2864 1870 Size: 73 313 608 4545 Fragments arranged by size: 4545 608 313 73 BamHI G'GATC_C Cuts at: 2869 3236 2869 Size: 367 5172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2410 2860 4315 618 Size: 325 217 627 623 450 1455 1842 Fragments arranged by size: 1842 1455 627 623 450 325 217 BanII G_rGCy'C Cuts at: 1391 2801 2858 1391 Size: 1410 57 4072 Fragments arranged by size: 4072 1410 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2330 2364 2551 2585 3730 3764 1903 Size: 34 393 34 187 34 1145 34 3678 Fragments arranged by size: 3678 1145 393 187 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2203 3431 4641 2203 Size: 1228 1210 3101 Fragments arranged by size: 3101 1228 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 136 Size: 107 122 71 631 605 2478 1525 Fragments arranged by size: 2478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5534 5534 Size: 5539 BlpI GC'TnA_GC Cuts at: 2573 2956 2573 Size: 383 5156 Bme1580I G_kGCm'C Cuts at: 1790 2415 2625 3600 4756 1790 Size: 625 210 975 1156 2573 Fragments arranged by size: 2573 1156 975 625 210 BmgBI CAC'GTC Cuts at: 1318 2077 1318 Size: 759 4780 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4228 442 Size: 416 53 83 399 24 1853 958 1753 Fragments arranged by size: 1853 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4199 1239 Size: 2960 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 2654 3664 4529 4827 2033 Size: 621 1010 865 298 2745 Fragments arranged by size: 2745 1010 865 621 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4202 882 Size: 617 2703 2219 Fragments arranged by size: 2703 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3330 493 Size: 711 2126 2702 Fragments arranged by size: 2702 2126 711 BsaBI GATnn'nnATC Cuts at: 5533 5533 Size: 5539 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3346 3728 4363 275 Size: 53 83 186 936 1813 382 635 1451 Fragments arranged by size: 1813 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 4370 4644 4791 5202 3972 Size: 398 274 147 411 4309 Fragments arranged by size: 4309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5539 BseYI C'CCAG_C Cuts at: 4742 5238 4742 Size: 496 5043 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 2596 2797 1163 Size: 573 636 224 201 3905 Fragments arranged by size: 3905 636 573 224 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2561 2604 2989 3750 4778 Size: 275 186 120 440 43 385 761 1028 Cuts at: 4778 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 440 385 275 186 120 43 BsiHKAI G_wGCw'C Cuts at: 2625 3600 3685 4756 2625 Size: 975 85 1071 3408 Fragments arranged by size: 3408 1071 975 85 BsiWI C'GTAC_G Cuts at: 2130 2130 Size: 5539 BsmI GAATG_Cn' Cuts at: 2704 3050 3143 2704 Size: 346 93 5100 Fragments arranged by size: 5100 346 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2625 2801 2858 3600 3685 4756 Size: 399 625 210 176 57 742 85 1071 Cuts at: 4756 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 625 399 210 176 85 57 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 3428 5158 3428 Size: 1730 3809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2398 2894 844 Size: 1554 496 3489 Fragments arranged by size: 3489 1554 496 BsrBI CCG'CTC Cuts at: 1394 1823 2247 2644 3426 1394 Size: 429 424 397 782 3507 Fragments arranged by size: 3507 782 429 424 397 BsrDI GCAATG_nn' Cuts at: 65 1248 2289 2649 4034 4208 65 Size: 1183 1041 360 1385 174 1396 Fragments arranged by size: 1396 1385 1183 1041 360 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2547 2617 4183 4370 1700 Size: 110 737 70 1566 187 2869 Fragments arranged by size: 2869 1566 737 187 110 70 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5539 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5539 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5539 BstBI TT'CG_AA Cuts at: 4343 4343 Size: 5539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5539 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2371 2176 Size: 195 5344 BstYI r'GATC_y Cuts at: 1972 2091 2367 2869 3236 3621 3638 5090 5176 Size: 119 276 502 367 385 17 1452 86 Cuts at: 5176 5188 5534 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 502 385 367 346 276 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2415 513 Size: 552 1350 3637 Fragments arranged by size: 3637 1350 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4800 Fragments arranged by size: 4800 683 56 BtsI GCAGTG_nn' Cuts at: 3055 3849 3869 3055 Size: 794 20 4725 Fragments arranged by size: 4725 794 20 ClaI AT'CG_AT Cuts at: 1554 2323 2340 1554 Size: 769 17 4753 Fragments arranged by size: 4753 769 17 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DraIII CAC_nnn'GTG Cuts at: 2270 2629 2270 Size: 359 5180 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2134 2514 Size: 54 1425 50 275 18 288 16 380 Cuts at: 2514 2558 2685 2986 3875 5504 8 Size: 44 127 301 889 1629 43 Fragments arranged by size: 1629 1425 889 380 301 288 275 127 54 50 44 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2118 2558 2986 1537 Size: 275 306 440 428 4090 Fragments arranged by size: 4090 440 428 306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3469 1353 Size: 2116 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2774 4126 4500 4646 387 Size: 1064 138 1185 1352 374 146 1280 Fragments arranged by size: 1352 1280 1185 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 3602 4199 4986 5254 1239 Size: 393 567 1403 597 787 268 1524 Fragments arranged by size: 1524 1403 787 597 567 393 268 EcoRI G'AATT_C Cuts at: 2848 3226 2848 Size: 378 5161 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 2013 2446 4686 1678 Size: 335 433 2240 2531 Fragments arranged by size: 2531 2240 433 335 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373 Size: 32 135 32 2608 32 42 32 2626 Fragments arranged by size: 2626 2608 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2883 3129 669 Size: 988 281 945 246 3079 Fragments arranged by size: 3079 988 945 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5539 HpaI GTT'AAC Cuts at: 3129 3129 Size: 5539 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4653 4837 1305 Size: 3348 184 2007 Fragments arranged by size: 3348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 2687 10 Size: 54 1425 343 684 171 2862 Fragments arranged by size: 2862 1425 684 343 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 3497 3856 4015 518 Size: 648 1452 879 359 159 2042 Fragments arranged by size: 2042 1452 879 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2301 2826 2955 3632 4780 5025 1606 Size: 695 525 129 677 1148 245 2120 Fragments arranged by size: 2120 1148 695 677 525 245 129 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4987 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2351 2897 4442 1132 Size: 97 1122 546 1545 2229 Fragments arranged by size: 2229 1545 1122 546 97 PasI CC'CwG_GG Cuts at: 1348 2014 1348 Size: 666 4873 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5539 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PstI C_TGCA'G Cuts at: 830 2109 2202 2891 830 Size: 1279 93 689 3478 Fragments arranged by size: 3478 1279 689 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 PvuII CAG'CTG Cuts at: 2826 2826 Size: 5539 RsrII CG'GwC_CG Cuts at: 2026 2026 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SbfI CC_TGCA'GG Cuts at: 2109 2891 2109 Size: 782 4757 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SexAI A'CCwGG_T Cuts at: 2625 2625 Size: 5539 SfcI C'TryA_G Cuts at: 826 1045 2105 2198 2887 4022 4703 4894 826 Size: 219 1060 93 689 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 689 681 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 2617 1700 Size: 917 4622 SmaI CCC'GGG Cuts at: 1872 2258 2866 1872 Size: 386 608 4545 Fragments arranged by size: 4545 608 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 2656 2669 3643 4544 4806 Size: 197 926 105 608 13 974 901 262 Cuts at: 4806 820 Size: 1553 Fragments arranged by size: 1553 974 926 901 608 262 197 105 13 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2351 2897 1132 Size: 1219 546 3774 Fragments arranged by size: 3774 1219 546 SspI AAT'ATT Cuts at: 5 52 3463 5 Size: 47 3411 2081 Fragments arranged by size: 3411 2081 47 StuI AGG'CCT Cuts at: 1517 2432 1517 Size: 915 4624 StyI C'CwwG_G Cuts at: 513 1065 2978 513 Size: 552 1913 3074 Fragments arranged by size: 3074 1913 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2762 3566 3583 3736 3921 4366 325 Size: 1255 1182 804 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1182 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3785 5497 96 Size: 274 80 33 51 1443 1808 1712 138 Fragments arranged by size: 1808 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2657 2689 2705 2737 5499 5531 2657 Size: 32 16 32 2762 32 2665 Fragments arranged by size: 2762 2665 32 32 32 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5539 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2173 2371 2011 Size: 162 198 5179 Fragments arranged by size: 5179 198 162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2256 2864 1870 Size: 386 608 4545 Fragments arranged by size: 4545 608 386 XmnI GAAnn'nnTTC Cuts at: 3668 3668 Size: 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SfiI SfoI SrfI SwaI