Restriction map for
pFN21AE3109
(Linear) (Six-base) MAPSORT of: KIEE3109.seq Check: 2537 from: 1 to: 834
KIEE3109.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 393 834
Size: 393 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 834
Size: 194 640
AflII C'TTAA_G
Cuts at: 0 651 834
Size: 651 183
AleI CACnn'nnGTG
Cuts at: 0 613 834
Size: 613 221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 834
Size: 631 203
ApaLI G'TGCA_C
Cuts at: 0 616 834
Size: 616 218
ApoI r'AATT_y
Cuts at: 0 827 834
Size: 827 7
AvaI C'yCGr_G
Cuts at: 0 251 834
Size: 251 583
BanI G'GyrC_C
Cuts at: 0 405 834
Size: 405 429
BanII G_rGCy'C
Cuts at: 0 796 834
Size: 796 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 359 546 580 834
Size: 325 34 187 34 254
Fragments arranged by size:
325 254 187 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 198 834
Size: 198 636
BlpI GC'TnA_GC
Cuts at: 0 568 834
Size: 568 266
Bme1580I G_kGCm'C
Cuts at: 0 410 620 834
Size: 410 210 214
Fragments arranged by size:
410 214 210
BmgBI CAC'GTC
Cuts at: 0 72 834
Size: 72 762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 649 834
Size: 28 621 185
Fragments arranged by size:
621 185 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 591 792 834
Size: 367 224 201 42
Fragments arranged by size:
367 224 201 42
BsiEI CG_ry'CG
Cuts at: 0 116 556 599 834
Size: 116 440 43 235
Fragments arranged by size:
440 235 116 43
BsiHKAI G_wGCw'C
Cuts at: 0 620 834
Size: 620 214
BsiWI C'GTAC_G
Cuts at: 0 125 834
Size: 125 709
BsmI GAATG_Cn'
Cuts at: 0 699 834
Size: 699 135
Bsp1286I G_dGCh'C
Cuts at: 0 410 620 796 834
Size: 410 210 176 38
Fragments arranged by size:
410 210 176 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 393 834
Size: 393 441
BsrBI CCG'CTC
Cuts at: 0 242 639 834
Size: 242 397 195
Fragments arranged by size:
397 242 195
BsrDI GCAATG_nn'
Cuts at: 0 284 644 834
Size: 284 360 190
Fragments arranged by size:
360 284 190
BsrFI r'CCGG_y
Cuts at: 0 542 612 834
Size: 542 70 222
Fragments arranged by size:
542 222 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 366 834
Size: 171 195 468
Fragments arranged by size:
468 195 171
BstYI r'GATC_y
Cuts at: 0 86 362 834
Size: 86 276 472
Fragments arranged by size:
472 276 86
BtgI C'CryG_G
Cuts at: 0 410 834
Size: 410 424
ClaI AT'CG_AT
Cuts at: 0 318 335 834
Size: 318 17 499
Fragments arranged by size:
499 318 17
DraIII CAC_nnn'GTG
Cuts at: 0 265 624 834
Size: 265 359 210
Fragments arranged by size:
359 265 210
EaeI y'GGCC_r
Cuts at: 0 113 129 509 553 680 834
Size: 113 16 380 44 127 154
Fragments arranged by size:
380 154 127 113 44 16
EagI C'GGCC_G
Cuts at: 0 113 553 834
Size: 113 440 281
Fragments arranged by size:
440 281 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 769 834
Size: 769 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 834
Size: 194 640
HaeII r_GCGC'y
Cuts at: 0 8 441 834
Size: 8 433 393
Fragments arranged by size:
433 393 8
MscI TGG'CCA
Cuts at: 0 511 682 834
Size: 511 171 152
Fragments arranged by size:
511 171 152
MslI CAynn'nnrTG
Cuts at: 0 613 834
Size: 613 221
MspA1I CmG'CkG
Cuts at: 0 296 821 834
Size: 296 525 13
Fragments arranged by size:
525 296 13
NspI r_CATG'y
Cuts at: 0 346 834
Size: 346 488
PasI CC'CwG_GG
Cuts at: 0 9 834
Size: 9 825
PstI C_TGCA'G
Cuts at: 0 104 197 834
Size: 104 93 637
Fragments arranged by size:
637 104 93
PvuII CAG'CTG
Cuts at: 0 821 834
Size: 821 13
RsrII CG'GwC_CG
Cuts at: 0 21 834
Size: 21 813
SbfI CC_TGCA'GG
Cuts at: 0 104 834
Size: 104 730
SexAI A'CCwGG_T
Cuts at: 0 620 834
Size: 620 214
SfcI C'TryA_G
Cuts at: 0 100 193 834
Size: 100 93 641
Fragments arranged by size:
641 100 93
SgrAI Cr'CCGG_yG
Cuts at: 0 612 834
Size: 612 222
SmaI CCC'GGG
Cuts at: 0 253 834
Size: 253 581
SmlI C'TyrA_G
Cuts at: 0 43 651 664 834
Size: 43 608 13 170
Fragments arranged by size:
608 170 43 13
SphI G_CATG'C
Cuts at: 0 346 834
Size: 346 488
StuI AGG'CCT
Cuts at: 0 427 834
Size: 427 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 757 834
Size: 757 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 652 684 700 732 834
Size: 652 32 16 32 102
Fragments arranged by size:
652 102 32 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 366 834
Size: 168 198 468
Fragments arranged by size:
468 198 168
XmaI C'CCGG_G
Cuts at: 0 251 834
Size: 251 583
Enzymes that do cut:
AarI AcuI AflII AleI AlfI ApaLI ApoI AvaI
BanI BanII BcgI BciVI BlpI Bme1580I BmgBI BpuEI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstXI BstYI BtgI ClaI DraIII EaeI
EagI EciI Eco57MI HaeII MscI MslI MspA1I NspI
PasI PstI PvuII RsrII SbfI SexAI SfcI SgrAI
SmaI SmlI SphI StuI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BclI BfrBI BglI BglII
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DrdI EarI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI SacI
SacII SalI SanDI SapI ScaI SfiI SfoI SgfI
SnaBI SpeI SrfI SspI StyI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI