################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 316 GGCGCC G[GA]^CG[CT]C AflIII 344 ACATGT A^C[GA][CT]GT AlwNI 598 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 80 GTGCTC G[AT]GC[AT]^C BalI 27 TGGCCA TGG^CCA BbeI 316 GGCGCC GGCGC^C BbsI 466 GTCTTC GAAGAC(2/6) BclI 308 TGATCA T^GATCA BfaI 553 CTAG C^TAG BpuAI 466 GTCTTC GAAGAC(2/6) BsaHI 316 GGCGCC G[GA]^CG[CT]C BsiHKAI 80 GTGCTC G[AT]GC[AT]^C BsmAI 127 GTCTC GTCTC(1/5) BsmI 19 GCATTC GAATGC(1/-1) Bsp1407I 341 TGTACA T^GTACA BspLU11I 344 ACATGT A^CATGT BspMI 7 ACCTGC ACCTGC(4/8) BsrBI 584 GAGCGG CCGCTC(-3/-3) BsrGI 341 TGTACA T^GTACA BssSI 355 CTCGTG CACGAG(-5/-1) BstF5I 471 CATCC GGATG(2/0) BstXI 39 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 309 GATC ^GATC DraIII 352 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 210 CCACGG C^C[GA][CT]GG EaeI 27 TGGCCA [CT]^GGCC[GA] EcoNI 500 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 586 GCGGG CCCGC(4/6) FbaI 308 TGATCA T^GATCA FokI 471 CATCC GGATG(9/13) Hin1I 316 GGCGCC G[GA]^CG[CT]C HincII 190 GTCAAC GT[CT]^[GA]AC HindII 190 GTCAAC GT[CT]^[GA]AC KasI 316 GGCGCC G^GCGCC MaeI 553 CTAG C^TAG MaeII 32 ACGT A^CGT MboI 309 GATC ^GATC MluNI 27 TGGCCA TGG^CCA MscI 27 TGGCCA TGG^CCA MslI 150 CACCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 316 GGCGCC GG^CGCC NdeI 728 CATATG CA^TATG NdeII 309 GATC ^GATC NspI 344 ACATGT [GA]CATG^[CT] PpuMI 49 GGGACCC [GA]G^G[AT]CC[CT] PvuII 301 CAGCTG CAG^CTG Sau3AI 309 GATC ^GATC SfoI 316 GGCGCC GGC^GCC SmlI 75 CTTGAG C^T[CT][GA]AG SspBI 341 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 342 GTAC GT^AC 504 GTAC Asp700I 537 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 540 GAAGTTCTTC AvaI 260 CCCGGG C^[CT]CG[GA]G 320 CCCGGG AvaII 50 GGACC G^G[AT]CC 629 GGACC BsgI 111 CTGCAC GTGCAG(16/14) 404 GTGCAG BsoBI 260 CCCGGG C^[CT]CG[GA]G 320 CCCGGG CfoI 224 GCGC GCG^C 317 GCGC EcoT14I 45 CCTTGG C^C[AT][AT]GG 652 CCAAGG HaeII 223 AGCGCC [GA]GCGC^[CT] 316 GGCGCC HhaI 224 GCGC GCG^C 317 GCGC HinP1I 224 GCGC G^CGC 317 GCGC HinfI 325 GACTC G^A[ACGT]TC 637 GACTC HphI 306 GGTGA GGTGA(8/7) 682 TCACC MaeIII 385 GTCAC ^GT[ACGT]AC 457 GTCAC PleI 325 GACTC GAGTC(4/5) 637 GACTC RsaI 342 GTAC GT^AC 504 GTAC SmaI 260 CCCGGG CCC^GGG 320 CCCGGG StyI 45 CCTTGG C^C[AT][AT]GG 652 CCAAGG Tsp45I 385 GTCAC ^GT[GC]AC 457 GTCAC XmaCI 260 CCCGGG C^CCGGG 320 CCCGGG XmaI 260 CCCGGG C^CCGGG 320 CCCGGG XmnI 537 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 540 GAAGTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI