(Circular) (Six-base) MAPSORT of: pFN21AE3084.seq Check: 2126 from: 1 to: 5449 pFN21AE3084 5449 bp With 182 enzymes: SgfI * March 28, 2012 13:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2792 1937 Size: 855 4594 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3512 4896 5164 1632 Size: 1880 1384 268 1917 Fragments arranged by size: 1917 1880 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 2349 4348 5325 1203 Size: 1146 1999 977 1327 Fragments arranged by size: 1999 1327 1146 977 AgeI A'CCGG_T Cuts at: 4280 4280 Size: 5449 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2531 2563 2531 Size: 32 5417 AlwNI CAG_nnn'CTG Cuts at: 2608 3192 4764 2608 Size: 584 1572 3293 Fragments arranged by size: 3293 1572 584 ApaLI G'TGCA_C Cuts at: 3506 4662 3506 Size: 1156 4293 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2265 2325 2774 1870 Size: 73 322 60 449 4545 Fragments arranged by size: 4545 449 322 73 60 BamHI G'GATC_C Cuts at: 2779 3146 2779 Size: 367 5082 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2321 2355 2514 2770 4225 Size: 325 217 627 534 34 159 256 1455 Cuts at: 4225 618 Size: 1842 Fragments arranged by size: 1842 1455 627 534 325 256 217 159 34 BanII G_rGCy'C Cuts at: 1391 2599 2652 2701 2768 1391 Size: 1208 53 49 67 4072 Fragments arranged by size: 4072 1208 67 53 49 BbeI G_GCGC'C Cuts at: 2325 2325 Size: 5449 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2464 928 Size: 971 565 3913 Fragments arranged by size: 3913 971 565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3640 3674 1903 Size: 34 50 34 1619 34 3678 Fragments arranged by size: 3678 1619 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 4684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4060 136 Size: 107 122 71 631 605 2388 1525 Fragments arranged by size: 2388 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5444 5444 Size: 5449 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 2215 3510 4666 1790 Size: 425 1295 1156 2573 Fragments arranged by size: 2573 1295 1156 425 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5449 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2413 2445 2741 Size: 416 53 83 399 24 996 32 296 Cuts at: 2741 3180 4138 442 Size: 439 958 1753 Fragments arranged by size: 1753 996 958 439 416 399 296 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2235 2267 2417 2723 4109 1239 Size: 996 32 150 306 1386 2579 Fragments arranged by size: 2579 1386 996 306 150 32 Bpu10I CC'TnA_GC Cuts at: 2609 2609 Size: 5449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2101 3574 4439 4737 2101 Size: 1473 865 298 2813 Fragments arranged by size: 2813 1473 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4112 882 Size: 617 2613 2219 Fragments arranged by size: 2613 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3240 493 Size: 711 2036 2702 Fragments arranged by size: 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 3256 3638 4273 Size: 53 83 186 936 789 934 382 635 Cuts at: 4273 275 Size: 1451 Fragments arranged by size: 1451 936 934 789 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3882 4280 4554 4701 5112 3882 Size: 398 274 147 411 4219 Fragments arranged by size: 4219 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2531 2561 2696 Size: 30 165 30 768 30 330 30 135 Cuts at: 2696 2726 1178 Size: 30 3901 Fragments arranged by size: 3901 768 330 165 135 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5449 BseYI C'CCAG_C Cuts at: 2100 2339 2526 2598 4652 5148 2100 Size: 239 187 72 2054 496 2401 Fragments arranged by size: 2401 2054 496 239 187 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2101 2430 1163 Size: 573 365 329 4182 Fragments arranged by size: 4182 573 365 329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3660 4688 1540 Size: 275 186 898 761 1028 2301 Fragments arranged by size: 2301 1028 898 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2089 3510 3595 4666 2089 Size: 1421 85 1071 2872 Fragments arranged by size: 2872 1421 1071 85 BsmI GAATG_Cn' Cuts at: 2024 2960 3053 2024 Size: 936 93 4420 Fragments arranged by size: 4420 936 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2215 2599 2652 2701 2768 3510 Size: 399 299 126 384 53 49 67 742 Cuts at: 3510 3595 4666 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 384 299 126 85 67 53 49 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 3338 5068 3338 Size: 1730 3719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 2804 844 Size: 1177 783 3489 Fragments arranged by size: 3489 1177 783 BsrBI CCG'CTC Cuts at: 1394 1823 2591 3336 1394 Size: 429 768 745 3507 Fragments arranged by size: 3507 768 745 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3944 4118 65 Size: 1183 2696 174 1396 Fragments arranged by size: 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4093 4280 1700 Size: 110 2283 187 2869 Fragments arranged by size: 2869 2283 187 110 BsrGI T'GTAC_A Cuts at: 96 2346 96 Size: 2250 3199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 2360 3509 4521 2360 Size: 1149 1012 3288 Fragments arranged by size: 3288 1149 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5449 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstXI CCAn_nnnn'nTGG Cuts at: 2051 2051 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444 Size: 807 367 385 17 1452 86 12 346 Cuts at: 5444 1972 Size: 1977 Fragments arranged by size: 1977 1452 807 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2215 513 Size: 552 1150 3747 Fragments arranged by size: 3747 1150 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4710 Fragments arranged by size: 4710 683 56 BtsI GCAGTG_nn' Cuts at: 2463 2965 3759 3779 2463 Size: 502 794 20 4133 Fragments arranged by size: 4133 794 502 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DraIII CAC_nnn'GTG Cuts at: 2362 2362 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2896 3785 Size: 54 1425 50 275 18 202 864 889 Cuts at: 3785 5414 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 864 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 1537 Size: 275 1084 4090 Fragments arranged by size: 4090 1084 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3379 1353 Size: 2026 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2413 4036 4410 4556 387 Size: 1064 138 824 1623 374 146 1280 Fragments arranged by size: 1623 1280 1064 824 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2267 2417 2723 3512 4109 4896 Size: 393 603 32 150 306 789 597 787 Cuts at: 4896 5164 1239 Size: 268 1524 Fragments arranged by size: 1524 789 787 603 597 393 306 268 150 32 EcoNI CCTnn'n_nnAGG Cuts at: 2509 2509 Size: 5449 EcoO109I rG'GnC_Cy Cuts at: 2047 2055 2605 2047 Size: 8 550 4891 Fragments arranged by size: 4891 550 8 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 5449 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 2232 2325 4596 1678 Size: 554 93 2271 2531 Fragments arranged by size: 2531 2271 554 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2696 2728 4090 4122 4164 Size: 32 135 32 1124 32 1362 32 42 Cuts at: 4164 4196 1373 Size: 32 2626 Fragments arranged by size: 2626 1362 1124 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 2793 3039 669 Size: 988 281 259 596 246 3079 Fragments arranged by size: 3079 988 596 281 259 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5449 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KasI G'GCGC_C Cuts at: 2321 2321 Size: 5449 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 4563 4747 1305 Size: 897 2361 184 2007 Fragments arranged by size: 2361 2007 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2034 10 Size: 54 1425 343 202 3425 Fragments arranged by size: 3425 1425 343 202 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 3407 3766 3925 518 Size: 648 993 1248 359 159 2042 Fragments arranged by size: 2042 1248 993 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2456 2520 2624 2665 2865 3542 4690 Size: 702 148 64 104 41 200 677 1148 Cuts at: 4690 4935 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 702 677 245 200 148 104 64 41 NarI GG'CG_CC Cuts at: 2322 2322 Size: 5449 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4897 NdeI CA'TA_TG Cuts at: 387 2734 387 Size: 2347 3102 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2353 2807 4352 1132 Size: 97 1124 454 1545 2229 Fragments arranged by size: 2229 1545 1124 454 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 2349 4348 2349 Size: 1999 3450 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2531 2563 3518 3550 5031 5063 2531 Size: 32 955 32 1481 32 2917 Fragments arranged by size: 2917 1481 955 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 2055 Size: 5449 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PstI C_TGCA'G Cuts at: 830 2801 830 Size: 1971 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 PvuII CAG'CTG Cuts at: 2308 2308 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SanDI GG'GwC_CC Cuts at: 2055 2055 Size: 5449 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2797 3932 4613 4804 826 Size: 219 1752 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2323 2323 Size: 5449 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5449 SmaI CCC'GGG Cuts at: 1872 2267 2327 2776 1872 Size: 395 60 449 4545 Fragments arranged by size: 4545 449 395 60 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 3553 4454 4716 820 Size: 197 926 137 1473 901 262 1553 Fragments arranged by size: 1553 1473 926 901 262 197 137 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2807 1132 Size: 1675 3774 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2050 2657 2888 513 Size: 552 985 607 231 3074 Fragments arranged by size: 3074 985 607 552 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 3695 5407 Size: 274 80 33 51 1443 369 1349 1712 Cuts at: 5407 96 Size: 138 Fragments arranged by size: 1712 1443 1349 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5409 5441 5409 Size: 32 5417 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2265 2325 2774 1870 Size: 395 60 449 4545 Fragments arranged by size: 4545 449 395 60 XmnI GAAnn'nnTTC Cuts at: 2546 2549 3578 2546 Size: 3 1029 4417 Fragments arranged by size: 4417 1029 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI