(Linear) (Six-base) MAPSORT of: KIEE3084.seq Check: 7830 from: 1 to: 744 KIEE3084.orf With 182 enzymes: SgfI * March 22, 2012 16:12 .. AflIII A'CryG_T Cuts at: 0 344 744 Size: 344 400 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 526 558 744 Size: 526 32 186 Fragments arranged by size: 526 186 32 AlwNI CAG_nnn'CTG Cuts at: 0 603 744 Size: 603 141 AvaI C'yCGr_G Cuts at: 0 260 320 744 Size: 260 60 424 Fragments arranged by size: 424 260 60 BanI G'GyrC_C Cuts at: 0 316 350 509 744 Size: 316 34 159 235 Fragments arranged by size: 316 235 159 34 BanII G_rGCy'C Cuts at: 0 594 647 696 744 Size: 594 53 49 48 Fragments arranged by size: 594 53 49 48 BbeI G_GCGC'C Cuts at: 0 320 744 Size: 320 424 BbsI GAAGACnn'nnnn_ Cuts at: 0 459 744 Size: 459 285 BclI T'GATC_A Cuts at: 0 308 744 Size: 308 436 Bme1580I G_kGCm'C Cuts at: 0 210 744 Size: 210 534 BmrI ACTGGGnnnn_n' Cuts at: 0 408 440 744 Size: 408 32 304 Fragments arranged by size: 408 304 32 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 230 262 412 718 744 Size: 230 32 150 306 26 Fragments arranged by size: 306 230 150 32 26 Bpu10I CC'TnA_GC Cuts at: 0 604 744 Size: 604 140 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 744 Size: 96 648 BsaHI Gr'CG_yC Cuts at: 0 317 744 Size: 317 427 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 166 196 526 556 691 721 744 Size: 166 30 330 30 135 30 23 Fragments arranged by size: 330 166 135 30 30 30 23 BseYI C'CCAG_C Cuts at: 0 95 334 521 593 744 Size: 95 239 187 72 151 Fragments arranged by size: 239 187 151 95 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 425 744 Size: 96 329 319 Fragments arranged by size: 329 319 96 BsiHKAI G_wGCw'C Cuts at: 0 84 744 Size: 84 660 BsmI GAATG_Cn' Cuts at: 0 19 744 Size: 19 725 Bsp1286I G_dGCh'C Cuts at: 0 84 210 594 647 696 744 Size: 84 126 384 53 49 48 Fragments arranged by size: 384 126 84 53 49 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 16 744 Size: 16 728 BsrBI CCG'CTC Cuts at: 0 586 744 Size: 586 158 BsrGI T'GTAC_A Cuts at: 0 341 744 Size: 341 403 BssSI C'ACGA_G Cuts at: 0 355 744 Size: 355 389 BstXI CCAn_nnnn'nTGG Cuts at: 0 46 744 Size: 46 698 BtgI C'CryG_G Cuts at: 0 210 744 Size: 210 534 BtsI GCAGTG_nn' Cuts at: 0 458 744 Size: 458 286 DraIII CAC_nnn'GTG Cuts at: 0 357 744 Size: 357 387 EaeI y'GGCC_r Cuts at: 0 27 744 Size: 27 717 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 408 744 Size: 408 336 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 230 262 412 718 744 Size: 230 32 150 306 26 Fragments arranged by size: 306 230 150 32 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 504 744 Size: 504 240 EcoO109I rG'GnC_Cy Cuts at: 0 42 50 600 744 Size: 42 8 550 144 Fragments arranged by size: 550 144 42 8 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 561 744 Size: 561 183 HaeII r_GCGC'y Cuts at: 0 227 320 744 Size: 227 93 424 Fragments arranged by size: 424 227 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 691 723 744 Size: 691 32 21 Fragments arranged by size: 691 32 21 HincII GTy'rAC Cuts at: 0 192 744 Size: 192 552 KasI G'GCGC_C Cuts at: 0 316 744 Size: 316 428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 197 744 Size: 197 547 MscI TGG'CCA Cuts at: 0 29 744 Size: 29 715 MslI CAynn'nnrTG Cuts at: 0 154 744 Size: 154 590 MspA1I CmG'CkG Cuts at: 0 303 451 515 619 660 744 Size: 303 148 64 104 41 84 Fragments arranged by size: 303 148 104 84 64 41 NarI GG'CG_CC Cuts at: 0 317 744 Size: 317 427 NdeI CA'TA_TG Cuts at: 0 729 744 Size: 729 15 NspI r_CATG'y Cuts at: 0 348 744 Size: 348 396 PciI A'CATG_T Cuts at: 0 344 744 Size: 344 400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 526 558 744 Size: 526 32 186 Fragments arranged by size: 526 186 32 PpuMI rG'GwC_Cy Cuts at: 0 50 744 Size: 50 694 PvuII CAG'CTG Cuts at: 0 303 744 Size: 303 441 SanDI GG'GwC_CC Cuts at: 0 50 744 Size: 50 694 SfoI GGC'GCC Cuts at: 0 318 744 Size: 318 426 SmaI CCC'GGG Cuts at: 0 262 322 744 Size: 262 60 422 Fragments arranged by size: 422 262 60 SmlI C'TyrA_G Cuts at: 0 75 744 Size: 75 669 StyI C'CwwG_G Cuts at: 0 45 652 744 Size: 45 607 92 Fragments arranged by size: 607 92 45 TatI w'GTAC_w Cuts at: 0 341 744 Size: 341 403 XmaI C'CCGG_G Cuts at: 0 260 320 744 Size: 260 60 424 Fragments arranged by size: 424 260 60 XmnI GAAnn'nnTTC Cuts at: 0 541 544 744 Size: 541 3 200 Fragments arranged by size: 541 200 3 Enzymes that do cut: AflIII AloI AlwNI AvaI BanI BanII BbeI BbsI BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrGI BssSI BstXI BtgI BtsI DraIII EaeI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NdeI NspI PciI PpiI PpuMI PvuII SanDI SfoI SmaI SmlI StyI TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI ZraI