Restriction map for
pFN21AE5319
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MseI MunI NciI NdeI NdeII
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI Sau3AI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI TaqI
TfiI Tru9I Tsp509I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 285 CGCG CG^CG
ApaI 617 GGGCCC GGGCC^C
AspHI 193 GTGCTC G[AT]GC[AT]^C
AspI 685 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 613 CTCGGG C^[CT]CG[GA]G
BbsI 849 GAAGAC GAAGAC(2/6)
BfaI 95 CTAG C^TAG
BlpI 456 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BpuAI 849 GAAGAC GAAGAC(2/6)
BsiEI 287 CGGCCG CG[GA][CT]^CG
BsiHKAI 193 GTGCTC G[AT]GC[AT]^C
BsmAI 172 GAGAC GTCTC(1/5)
BsmBI 172 GAGACG CGTCTC(1/5)
BsmI 541 GCATTC GAATGC(1/-1)
BsoBI 613 CTCGGG C^[CT]CG[GA]G
BspMI 825 GCAGGT ACCTGC(4/8)
BsrFI 289 GCCGGC [GA]^CCGG[CT]
BsrI 373 CCAGT ACTGG(1/-1)
BstUI 285 CGCG CG^CG
CelII 456 GCTGAGC GC^T[ACGT]AGC
Cfr10I 289 GCCGGC [GA]^CCGG[CT]
DraIII 268 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 891 GACCGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 809 CCATGG C^C[GA][CT]GG
EagI 287 CGGCCG C^GGCCG
EclXI 287 CGGCCG C^GGCCG
Eco52I 287 CGGCCG C^GGCCG
HapII 290 CCGG C^CGG
HincII 153 GTTGAC GT[CT]^[GA]AC
HindII 153 GTTGAC GT[CT]^[GA]AC
HinfI 877 GACTC G^A[ACGT]TC
HpaII 290 CCGG C^CGG
MaeI 95 CTAG C^TAG
MaeII 689 ACGT A^CGT
MspI 290 CCGG C^CGG
MvnI 285 CGCG CG^CG
NaeI 289 GCCGGC GCC^GGC
NcoI 809 CCATGG C^CATGG
NgoMIV 289 GCCGGC G^CCGGC
NheI 94 GCTAGC G^CTAGC
NspI 726 GCATGT [GA]CATG^[CT]
PflFI 685 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 877 GACTC GAGTC(4/5)
PpuMI 491 AGGACCT [GA]G^G[AT]CC[CT]
SapI 195 GCTCTTC GCTCTTC(1/4)
SbfI 883 CCTGCAGG CCTGCA^GG
SmlI 679 CTCAAG C^T[CT][GA]AG
Sse8387I 883 CCTGCAGG CCTGCA^GG
StuI 776 AGGCCT AGG^CCT
Tth111I 685 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 122 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
451 CAGCTGCTG
BanI 247 GGCGCC G^G[CT][GA]CC
596 GGCGCC
BanII 514 GGGCTC G[GA]GC[CT]^C
617 GGGCCC
BbeI 247 GGCGCC GGCGC^C
596 GGCGCC
BpmI 371 CTCCAG CTGGAG(16/14)
733 CTGGAG
BsmFI 585 GGGAC GGGAC(10/14)
889 GGGAC
BsrBI 750 CCGCTC CCGCTC(-3/-3)
893 CCGCTC
EarI 196 CTCTTC CTCTTC(1/4)
487 GAAGAG
EcoNI 42 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
858 CCTGGACAAGG
HgaI 68 GCGTC GACGC(5/10)
293 GCGTC
KasI 247 GGCGCC G^GCGCC
596 GGCGCC
Ksp632I 196 CTCTTC CTCTTC(1/4)
487 GAAGAG
NarI 247 GGCGCC GG^CGCC
596 GGCGCC
PstI 802 CTGCAG CTGCA^G
884 CTGCAG
PvuII 28 CAGCTG CAG^CTG
451 CAGCTG
SfcI 802 CTGCAG C^T[GA][CT]AG
884 CTGCAG
SfoI 247 GGCGCC GGC^GCC
596 GGCGCC
TspRI 186 CAGTG CA[GC]TG[ACGT][ACGT]^
519 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcyI AluI AvaII BalI
BbvI BmyI BsaHI BsaJI BsgI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HphI ItaI
MaeIII MboII MluNI MnlI MscI
MslI MspA1I MvaI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI StyI
TseI Tsp45I