(Circular) (Six-base) MAPSORT of: pFN21AE5319.seq Check: 1106 from: 1 to: 5662 pFN21AE5319 5662 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2821 2821 Size: 5662 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3005 1937 Size: 1068 4594 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3725 5109 5377 1632 Size: 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 4561 5538 1203 Size: 3358 977 1327 Fragments arranged by size: 3358 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2486 1166 Size: 1320 4342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AlwNI CAG_nnn'CTG Cuts at: 2132 2461 3405 4977 2132 Size: 329 944 1572 2817 Fragments arranged by size: 2817 1572 944 329 ApaI G_GGCC'C Cuts at: 2626 2626 Size: 5662 ApaLI G'TGCA_C Cuts at: 3719 4875 3719 Size: 1156 4506 ApoI r'AATT_y Cuts at: 1493 2971 3197 3349 5458 1493 Size: 1478 226 152 2109 1697 Fragments arranged by size: 2109 1697 1478 226 152 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2618 2987 1870 Size: 73 675 369 4545 Fragments arranged by size: 4545 675 369 73 BamHI G'GATC_C Cuts at: 2992 3359 2992 Size: 367 5295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 2601 2983 4438 618 Size: 325 217 627 465 349 382 1455 1842 Fragments arranged by size: 1842 1455 627 465 382 349 325 217 BanII G_rGCy'C Cuts at: 1391 2523 2626 2981 1391 Size: 1132 103 355 4072 Fragments arranged by size: 4072 1132 355 103 BbeI G_GCGC'C Cuts at: 2256 2605 2256 Size: 349 5313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 928 Size: 971 962 3729 Fragments arranged by size: 3729 971 962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3853 3887 1903 Size: 34 1916 34 3678 Fragments arranged by size: 3678 1916 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4764 3554 Size: 1210 4452 BclI T'GATC_A Cuts at: 1548 1548 Size: 5662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4273 136 Size: 107 122 71 631 605 2601 1525 Fragments arranged by size: 2601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5657 5657 Size: 5662 BlpI GC'TnA_GC Cuts at: 2462 3079 2462 Size: 617 5045 Bme1580I G_kGCm'C Cuts at: 1790 2626 3723 4879 1790 Size: 836 1097 1156 2573 Fragments arranged by size: 2573 1156 1097 836 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2103 1056 Size: 1047 4615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2361 2759 4322 1239 Size: 1122 398 1563 2579 Fragments arranged by size: 2579 1563 1122 398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2669 3787 4652 4950 2669 Size: 1118 865 298 3381 Fragments arranged by size: 3381 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4325 882 Size: 617 2826 2219 Fragments arranged by size: 2826 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3453 493 Size: 711 2249 2702 Fragments arranged by size: 2702 2249 711 BsaBI GATnn'nnATC Cuts at: 5656 5656 Size: 5662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2253 2298 2602 Size: 53 83 186 936 540 180 45 304 Cuts at: 2602 3469 3851 4486 275 Size: 867 382 635 1451 Fragments arranged by size: 1451 936 867 635 540 382 304 186 180 83 53 45 BsaWI w'CCGG_w Cuts at: 4095 4493 4767 4914 5325 4095 Size: 398 274 147 411 4432 Fragments arranged by size: 4432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2359 2389 2795 2819 2825 Size: 30 165 30 956 30 406 24 6 Cuts at: 2825 2849 1178 Size: 24 3991 Fragments arranged by size: 3991 956 406 165 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5662 BseYI C'CCAG_C Cuts at: 2947 4865 5361 2947 Size: 1918 496 3248 Fragments arranged by size: 3248 1918 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2464 2642 1163 Size: 573 468 260 178 4183 Fragments arranged by size: 4183 573 468 260 178 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2295 3112 3873 4901 1540 Size: 275 186 294 817 761 1028 2301 Fragments arranged by size: 2301 1028 817 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 3723 3808 4879 2202 Size: 1521 85 1071 2985 Fragments arranged by size: 2985 1521 1071 85 BsmI GAATG_Cn' Cuts at: 2546 3173 3266 2546 Size: 627 93 4942 Fragments arranged by size: 4942 627 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2171 2171 Size: 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2523 2626 2981 3723 3808 4879 Size: 399 412 321 103 355 742 85 1071 Cuts at: 4879 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 412 399 355 321 103 85 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2821 3017 844 Size: 1977 196 3489 Fragments arranged by size: 3489 1977 196 BsrBI CCG'CTC Cuts at: 1394 1823 2757 2900 3549 1394 Size: 429 934 143 649 3507 Fragments arranged by size: 3507 934 649 429 143 BsrDI GCAATG_nn' Cuts at: 65 1248 4157 4331 65 Size: 1183 2909 174 1396 Fragments arranged by size: 2909 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2294 4306 4493 1700 Size: 110 484 2012 187 2869 Fragments arranged by size: 2869 2012 484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5662 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5662 BstYI r'GATC_y Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657 Size: 1020 367 385 17 1452 86 12 346 Cuts at: 5657 1972 Size: 1977 Fragments arranged by size: 1977 1452 1020 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2814 513 Size: 552 1749 3361 Fragments arranged by size: 3361 1749 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3972 3992 3178 Size: 794 20 4848 Fragments arranged by size: 4848 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DraIII CAC_nnn'GTG Cuts at: 2278 2278 Size: 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2902 4669 809 Size: 507 1586 1767 1802 Fragments arranged by size: 1802 1767 1586 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2049 2121 Size: 54 1425 50 275 18 199 20 72 Cuts at: 2121 2292 2339 2914 3109 3998 5627 8 Size: 171 47 575 195 889 1629 43 Fragments arranged by size: 1629 1425 889 575 275 199 195 171 72 54 50 47 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2292 3109 1537 Size: 275 480 817 4090 Fragments arranged by size: 4090 817 480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2487 3592 1353 Size: 854 280 1105 3423 Fragments arranged by size: 3423 1105 854 280 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2603 4249 4623 4769 387 Size: 1064 138 1014 1646 374 146 1280 Fragments arranged by size: 1646 1280 1064 1014 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2361 2759 3725 4322 5109 5377 1239 Size: 393 729 398 966 597 787 268 1524 Fragments arranged by size: 1524 966 787 729 597 398 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2867 2051 Size: 816 4846 EcoO109I rG'GnC_Cy Cuts at: 2497 2622 2629 2497 Size: 125 7 5530 Fragments arranged by size: 5530 125 7 EcoRI G'AATT_C Cuts at: 2971 3349 2971 Size: 378 5284 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 2028 2256 2605 4809 1678 Size: 350 228 349 2204 2531 Fragments arranged by size: 2531 2204 350 349 228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2359 2391 2795 2819 2827 Size: 32 135 32 787 32 404 24 8 Cuts at: 2827 2851 4303 4335 4377 4409 1373 Size: 24 1452 32 42 32 2626 Fragments arranged by size: 2626 1452 787 404 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2160 3006 3252 669 Size: 988 281 222 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KasI G'GCGC_C Cuts at: 2252 2601 2252 Size: 349 5313 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 3261 3261 Size: 5662 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4776 4960 1305 Size: 3471 184 2007 Fragments arranged by size: 3471 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2031 2051 2123 2916 10 Size: 54 1425 343 199 20 72 793 2756 Fragments arranged by size: 2756 1425 793 343 199 72 54 20 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2193 2279 2486 3620 3979 4138 Size: 648 843 184 86 207 1134 359 159 Cuts at: 4138 518 Size: 2042 Fragments arranged by size: 2042 1134 843 648 359 207 184 159 86 MspA1I CmG'CkG Cuts at: 1606 2035 2361 2458 2532 2951 3078 3755 4903 Size: 429 326 97 74 419 127 677 1148 Cuts at: 4903 5148 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 429 419 326 245 127 97 74 NaeI GCC'GGC Cuts at: 2296 2296 Size: 5662 NarI GG'CG_CC Cuts at: 2253 2602 2253 Size: 349 5313 NcoI C'CATG_G Cuts at: 513 1065 2814 513 Size: 552 1749 3361 Fragments arranged by size: 3361 1749 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NgoMIV G'CCGG_C Cuts at: 2294 2294 Size: 5662 NheI G'CTAG_C Cuts at: 1052 2099 1052 Size: 1047 4615 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 2735 3020 4565 1132 Size: 97 1506 285 1545 2229 Fragments arranged by size: 2229 1545 1506 285 97 PasI CC'CwG_GG Cuts at: 1348 2349 1348 Size: 1001 4661 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5662 PfoI T'CCnGG_A Cuts at: 2238 2862 2238 Size: 624 5038 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2497 Size: 5662 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PspOMI G'GGCC_C Cuts at: 2622 2622 Size: 5662 PstI C_TGCA'G Cuts at: 830 2811 2893 3014 830 Size: 1981 82 121 3478 Fragments arranged by size: 3478 1981 121 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2035 2458 2035 Size: 423 5239 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SapI GCTCTTCn'nnn_ Cuts at: 2207 2207 Size: 5662 SbfI CC_TGCA'GG Cuts at: 2893 3014 2893 Size: 121 5541 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2807 2889 3010 4145 4826 5017 826 Size: 219 1762 82 121 1135 681 191 1471 Fragments arranged by size: 1762 1471 1135 681 219 191 121 82 SfoI GGC'GCC Cuts at: 2254 2603 2254 Size: 349 5313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2684 3766 4667 4929 820 Size: 197 926 741 1082 901 262 1553 Fragments arranged by size: 1553 1082 926 901 741 262 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 2783 1517 Size: 1266 4396 StyI C'CwwG_G Cuts at: 513 1065 2052 2597 2814 3101 513 Size: 552 987 545 217 287 3074 Fragments arranged by size: 3074 987 552 545 287 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2688 3689 3706 3859 4044 4489 325 Size: 1255 1108 1001 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1108 1001 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3908 5620 96 Size: 274 80 33 51 1443 1931 1712 138 Fragments arranged by size: 1931 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 5622 5654 2211 Size: 32 3379 32 2219 Fragments arranged by size: 3379 2219 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2693 1316 Size: 1377 4285 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI