Restriction map for
pFN21AE5319
(Linear) (Six-base) MAPSORT of: KIEE5319.seq Check: 4885 from: 1 to: 957
KIEE5319.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 816 957
Size: 816 141
AleI CACnn'nnGTG
Cuts at: 0 481 957
Size: 481 476
AlwNI CAG_nnn'CTG
Cuts at: 0 127 456 957
Size: 127 329 501
Fragments arranged by size:
501 329 127
ApaI G_GGCC'C
Cuts at: 0 621 957
Size: 621 336
AvaI C'yCGr_G
Cuts at: 0 613 957
Size: 613 344
BanI G'GyrC_C
Cuts at: 0 247 596 957
Size: 247 349 361
Fragments arranged by size:
361 349 247
BanII G_rGCy'C
Cuts at: 0 518 621 957
Size: 518 103 336
Fragments arranged by size:
518 336 103
BbeI G_GCGC'C
Cuts at: 0 251 600 957
Size: 251 349 357
Fragments arranged by size:
357 349 251
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 957
Size: 856 101
BlpI GC'TnA_GC
Cuts at: 0 457 957
Size: 457 500
Bme1580I G_kGCm'C
Cuts at: 0 621 957
Size: 621 336
BmtI G_CTAG'C
Cuts at: 0 98 957
Size: 98 859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 754 957
Size: 356 398 203
Fragments arranged by size:
398 356 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 957
Size: 664 293
BsaHI Gr'CG_yC
Cuts at: 0 68 248 293 597 957
Size: 68 180 45 304 360
Fragments arranged by size:
360 304 180 68 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 354 384 790 814 820 844 957
Size: 354 30 406 24 6 24 113
Fragments arranged by size:
406 354 113 30 24 24 6
BseYI C'CCAG_C
Cuts at: 0 942 957
Size: 942 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 459 637 957
Size: 199 260 178 320
Fragments arranged by size:
320 260 199 178
BsiEI CG_ry'CG
Cuts at: 0 290 957
Size: 290 667
BsiHKAI G_wGCw'C
Cuts at: 0 197 957
Size: 197 760
BsmI GAATG_Cn'
Cuts at: 0 541 957
Size: 541 416
BsmBI CGTCTCn'nnnn_
Cuts at: 0 166 957
Size: 166 791
Bsp1286I G_dGCh'C
Cuts at: 0 197 518 621 957
Size: 197 321 103 336
Fragments arranged by size:
336 321 197 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 816 957
Size: 816 141
BsrBI CCG'CTC
Cuts at: 0 752 895 957
Size: 752 143 62
Fragments arranged by size:
752 143 62
BsrFI r'CCGG_y
Cuts at: 0 289 957
Size: 289 668
BtgI C'CryG_G
Cuts at: 0 809 957
Size: 809 148
DraIII CAC_nnn'GTG
Cuts at: 0 273 957
Size: 273 684
DrdI GACnn_nn'nnGTC
Cuts at: 0 897 957
Size: 897 60
EaeI y'GGCC_r
Cuts at: 0 24 44 116 287 334 909 957
Size: 24 20 72 171 47 575 48
Fragments arranged by size:
575 171 72 48 47 24 20
EagI C'GGCC_G
Cuts at: 0 287 957
Size: 287 670
EarI CTCTTCn'nnn_
Cuts at: 0 202 482 957
Size: 202 280 475
Fragments arranged by size:
475 280 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 598 957
Size: 598 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 754 957
Size: 356 398 203
Fragments arranged by size:
398 356 203
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 862 957
Size: 46 816 95
Fragments arranged by size:
816 95 46
EcoO109I rG'GnC_Cy
Cuts at: 0 492 617 624 957
Size: 492 125 7 333
Fragments arranged by size:
492 333 125 7
HaeII r_GCGC'y
Cuts at: 0 23 251 600 957
Size: 23 228 349 357
Fragments arranged by size:
357 349 228 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 790 814 822 846 957
Size: 354 32 404 24 8 24 111
Fragments arranged by size:
404 354 111 32 24 24 8
HincII GTy'rAC
Cuts at: 0 155 957
Size: 155 802
KasI G'GCGC_C
Cuts at: 0 247 596 957
Size: 247 349 361
Fragments arranged by size:
361 349 247
MscI TGG'CCA
Cuts at: 0 26 46 118 911 957
Size: 26 20 72 793 46
Fragments arranged by size:
793 72 46 26 20
MslI CAynn'nnrTG
Cuts at: 0 188 274 481 957
Size: 188 86 207 476
Fragments arranged by size:
476 207 188 86
MspA1I CmG'CkG
Cuts at: 0 30 356 453 527 946 957
Size: 30 326 97 74 419 11
Fragments arranged by size:
419 326 97 74 30 11
NaeI GCC'GGC
Cuts at: 0 291 957
Size: 291 666
NarI GG'CG_CC
Cuts at: 0 248 597 957
Size: 248 349 360
Fragments arranged by size:
360 349 248
NcoI C'CATG_G
Cuts at: 0 809 957
Size: 809 148
NgoMIV G'CCGG_C
Cuts at: 0 289 957
Size: 289 668
NheI G'CTAG_C
Cuts at: 0 94 957
Size: 94 863
NspI r_CATG'y
Cuts at: 0 730 957
Size: 730 227
PasI CC'CwG_GG
Cuts at: 0 344 957
Size: 344 613
PfoI T'CCnGG_A
Cuts at: 0 233 857 957
Size: 233 624 100
Fragments arranged by size:
624 233 100
PpuMI rG'GwC_Cy
Cuts at: 0 492 957
Size: 492 465
PspOMI G'GGCC_C
Cuts at: 0 617 957
Size: 617 340
PstI C_TGCA'G
Cuts at: 0 806 888 957
Size: 806 82 69
Fragments arranged by size:
806 82 69
PvuII CAG'CTG
Cuts at: 0 30 453 957
Size: 30 423 504
Fragments arranged by size:
504 423 30
SapI GCTCTTCn'nnn_
Cuts at: 0 202 957
Size: 202 755
SbfI CC_TGCA'GG
Cuts at: 0 888 957
Size: 888 69
SfcI C'TryA_G
Cuts at: 0 802 884 957
Size: 802 82 73
Fragments arranged by size:
802 82 73
SfoI GGC'GCC
Cuts at: 0 249 598 957
Size: 249 349 359
Fragments arranged by size:
359 349 249
SmlI C'TyrA_G
Cuts at: 0 679 957
Size: 679 278
StuI AGG'CCT
Cuts at: 0 778 957
Size: 778 179
StyI C'CwwG_G
Cuts at: 0 47 592 809 957
Size: 47 545 217 148
Fragments arranged by size:
545 217 148 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 683 957
Size: 683 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 957
Size: 206 32 719
Fragments arranged by size:
719 206 32
Tth111I GACn'n_nGTC
Cuts at: 0 688 957
Size: 688 269
Enzymes that do cut:
AarI AleI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BlpI Bme1580I BmtI BpmI BpuEI BsaHI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BtgI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PfoI PpuMI PspOMI PstI PvuII SapI
SbfI SfcI SfoI SmlI StuI StyI TaqII TstI
Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BseRI BsiWI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NdeI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XcmI XhoI
XmaI XmnI ZraI