################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NarI NciI NcoI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 264 AACGTT AA^CGTT AfaI 177 GTAC GT^AC Alw44I 149 GTGCAC G^TGCAC AlwI 273 GATCC GGATC(4/5) AlwNI 193 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 149 GTGCAC G^TGCAC AvaII 201 GGTCC G^G[AT]CC BanII 250 GAGCTC G[GA]GC[CT]^C BbvCI 535 GCTGAGG CCTCAGC(-5/-2) BcgI 464 GCATCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 434 TGATCA T^GATCA BseRI 111 GAGGAG GAGGAG(10/8) BsiEI 11 CGGCCG CG[GA][CT]^CG BsrBI 14 CCGCTC CCGCTC(-3/-3) BsrFI 6 GCCGGC [GA]^CCGG[CT] BstYI 272 AGATCC [GA]^GATC[CT] Cfr10I 6 GCCGGC [GA]^CCGG[CT] EagI 11 CGGCCG C^GGCCG EclXI 11 CGGCCG C^GGCCG Eco52I 11 CGGCCG C^GGCCG EcoRV 358 GATATC GAT^ATC FbaI 434 TGATCA T^GATCA HapII 7 CCGG C^CGG HinfI 496 GAGTC G^A[ACGT]TC HpaII 7 CCGG C^CGG HphI 389 TCACC GGTGA(8/7) MaeII 265 ACGT A^CGT MflI 272 AGATCC [GA]^GATC[CT] MspI 7 CCGG C^CGG NaeI 6 GCCGGC GCC^GGC NdeI 106 CATATG CA^TATG NgoMIV 6 GCCGGC G^CCGGC NlaIII 86 CATG CATG^ NotI 10 GCGGCCGC GC^GGCCGC PleI 496 GAGTC GAGTC(4/5) Psp1406I 264 AACGTT AA^CGTT PstI 329 CTGCAG CTGCA^G PvuII 196 CAGCTG CAG^CTG RsaI 177 GTAC GT^AC SacI 250 GAGCTC GAGCT^C SfaNI 464 GCATC GCATC(5/9) SfcI 329 CTGCAG C^T[GA][CT]AG XhoII 272 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AspHI 149 GTGCAC G[AT]GC[AT]^C 250 GAGCTC BanI 81 GGTGCC G^G[CT][GA]CC 577 GGCACC BglI 337 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 559 GCCCTGGAGGC BmyI 149 GTGCAC G[AGT]GC[ACT]^C 250 GAGCTC BpmI 562 CTGGAG CTGGAG(16/14) 589 CTGGAG BsiHKAI 149 GTGCAC G[AT]GC[AT]^C 250 GAGCTC BsmFI 322 GTCCC GGGAC(10/14) 498 GTCCC Bsp1286I 149 GTGCAC G[AGT]GC[ACT]^C 250 GAGCTC BstF5I 456 CATCC GGATG(2/0) 279 GGATG DdeI 237 CTCAG C^T[ACGT]AG 536 CTGAG DraII 30 GGGGCCT [GA]G^G[ACGT]CC[CT] 137 AGGCCCT DsaI 416 CCGTGG C^C[GA][CT]GG 581 CCGTGG EaeI 11 CGGCCG [CT]^GGCC[GA] 120 CGGCCA EcoO109I 30 GGGGCCT [GA]G^G[ACGT]CC[CT] 137 AGGCCCT FokI 456 CATCC GGATG(9/13) 279 GGATG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DpnII EcoNI EcoRII EcoT14I Fnu4HI HaeIII ItaI MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI StyI TseI