################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BalI BanII BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaWI BseAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI MaeI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AluI 82 AGCT AG^CT Alw44I 94 GTGCAC G^TGCAC ApaLI 94 GTGCAC G^TGCAC AspI 526 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 654 GGACC G^G[AT]CC BamHI 203 GGATCC G^GATCC BclI 131 TGATCA T^GATCA BsaBI 363 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 297 GGTCTC GGTCTC(1/5) BsiEI 68 CGGCCG CG[GA][CT]^CG BsmAI 298 GTCTC GTCTC(1/5) BstF5I 521 GGATG GGATG(2/0) BstYI 203 GGATCC [GA]^GATC[CT] EaeI 68 CGGCCG [CT]^GGCC[GA] EagI 68 CGGCCG C^GGCCG EarI 423 GAAGAG CTCTTC(1/4) EclXI 68 CGGCCG C^GGCCG Eco52I 68 CGGCCG C^GGCCG FbaI 131 TGATCA T^GATCA FokI 521 GGATG GGATG(9/13) HinfI 166 GACTC G^A[ACGT]TC HphI 371 TCACC GGTGA(8/7) Ksp632I 423 GAAGAG CTCTTC(1/4) MaeII 308 ACGT A^CGT MamI 363 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 203 GGATCC [GA]^GATC[CT] NlaIII 480 CATG CATG^ PflFI 526 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 166 GACTC GAGTC(4/5) PpuMI 653 AGGACCC [GA]G^G[AT]CC[CT] PstI 78 CTGCAG CTGCA^G PvuII 81 CAGCTG CAG^CTG SapI 423 GAAGAGC GCTCTTC(1/4) SfcI 78 CTGCAG C^T[GA][CT]AG Tth111I 526 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 203 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 440 GGTACC G^GTACC 608 GGTACC AfaI 441 GTAC GT^AC 609 GTAC Asp718I 440 GGTACC G^GTACC 608 GGTACC AspHI 94 GTGCAC G[AT]GC[AT]^C 408 GAGCAC BbeI 4 GGCGCC GGCGC^C 621 GGCGCC BseRI 279 CTCCTC GAGGAG(10/8) 498 CTCCTC BsiHKAI 94 GTGCAC G[AT]GC[AT]^C 408 GAGCAC BsmFI 353 GGGAC GGGAC(10/14) 398 GGGAC BsrDI 333 CATTGC GCAATG(2/0) 116 GCAATG BsrI 527 ACTGG ACTGG(1/-1) 207 CCAGT BstNI 395 CCTGG CC^[AT]GG 558 CCAGG DdeI 245 CTGAG C^T[ACGT]AG 406 CTGAG DraII 240 AGGGCCT [GA]G^G[ACGT]CC[CT] 653 AGGACCC DsaI 8 CCGCGG C^C[GA][CT]GG 612 CCGCGG EcoO109I 240 AGGGCCT [GA]G^G[ACGT]CC[CT] 653 AGGACCC EcoRII 395 CCTGG ^CC[AT]GG 558 CCAGG FauI 14 CCCGC CCCGC(4/6) 99 CCCGC HgaI 54 GACGC GACGC(5/10) 73 GACGC KasI 4 GGCGCC G^GCGCC 621 GGCGCC KpnI 440 GGTACC GGTAC^C 608 GGTACC KspI 8 CCGCGG CCGC^GG 612 CCGCGG MvaI 395 CCTGG CC^[AT]GG 558 CCAGG NarI 4 GGCGCC GG^CGCC 621 GGCGCC RsaI 441 GTAC GT^AC 609 GTAC SacII 8 CCGCGG CCGC^GG 612 CCGCGG SfaNI 363 GATGC GCATC(5/9) 578 GCATC SfoI 4 GGCGCC GGC^GCC 621 GGCGCC TaqI 644 TCGA T^CGA 650 TCGA TspRI 150 CAGTG CA[GC]TG[ACGT][ACGT]^ 287 CAGTG TthHB8I 644 TCGA T^CGA 650 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AccII AciI AcyI AlwI BanI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstUI Cac8I CfoI Cfr13I DpnII Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I