(Circular) (Six-base) MAPSORT of: pFN21AE6627.seq Check: 3983 from: 1 to: 5365 pFN21AE6627 5365 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3175 278 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2708 1937 Size: 771 4594 Acc65I G'GTAC_C Cuts at: 2445 2613 2686 2445 Size: 168 73 5124 Fragments arranged by size: 5124 168 73 AclI AA'CG_TT Cuts at: 1563 3492 3865 1563 Size: 1929 373 3063 Fragments arranged by size: 3063 1929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3428 4812 5080 1632 Size: 1796 1384 268 1917 Fragments arranged by size: 1917 1796 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5168 AflIII A'CryG_T Cuts at: 1203 4264 5241 1203 Size: 3061 977 1327 Fragments arranged by size: 3061 1327 977 AgeI A'CCGG_T Cuts at: 4196 4196 Size: 5365 AhdI GACnn_n'nnGTC Cuts at: 4094 4094 Size: 5365 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5365 AlwNI CAG_nnn'CTG Cuts at: 3108 4680 3108 Size: 1572 3793 ApaLI G'TGCA_C Cuts at: 2099 3422 4578 2099 Size: 1323 1156 2886 Fragments arranged by size: 2886 1323 1156 ApoI r'AATT_y Cuts at: 1493 2674 2900 3052 5161 1493 Size: 1181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 226 152 AseI AT'TA_AT Cuts at: 160 1032 3919 160 Size: 872 2887 1606 Fragments arranged by size: 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 AvaI C'yCGr_G Cuts at: 1870 1943 2690 1870 Size: 73 747 4545 Fragments arranged by size: 4545 747 73 BamHI G'GATC_C Cuts at: 2208 2695 3062 2208 Size: 487 367 4511 Fragments arranged by size: 4511 487 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2445 2559 2613 2626 Size: 325 217 627 222 436 114 54 13 Cuts at: 2626 2686 4141 618 Size: 60 1455 1842 Fragments arranged by size: 1842 1455 627 436 325 222 217 114 60 54 13 BanII G_rGCy'C Cuts at: 1391 2684 1391 Size: 1293 4072 BbeI G_GCGC'C Cuts at: 2013 2630 2013 Size: 617 4748 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3556 3590 1903 Size: 34 1619 34 3678 Fragments arranged by size: 3678 1619 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3257 4467 3257 Size: 1210 4155 BclI T'GATC_A Cuts at: 1548 2136 1548 Size: 588 4777 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3976 136 Size: 107 122 71 631 605 2304 1525 Fragments arranged by size: 2304 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5360 5360 Size: 5365 BlpI GC'TnA_GC Cuts at: 2782 2782 Size: 5365 Bme1580I G_kGCm'C Cuts at: 1790 2103 2400 2562 3426 4582 1790 Size: 313 297 162 864 1156 2573 Fragments arranged by size: 2573 1156 864 313 297 162 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2542 2657 3096 Size: 416 53 83 399 24 1125 115 439 Cuts at: 3096 4054 442 Size: 958 1753 Fragments arranged by size: 1753 1125 958 439 416 399 115 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4025 1239 Size: 2786 2579 Bpu10I CC'TnA_GC Cuts at: 2411 2411 Size: 5365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3490 4355 4653 3490 Size: 865 298 4202 Fragments arranged by size: 4202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2308 4028 882 Size: 617 809 1720 2219 Fragments arranged by size: 2219 1720 809 617 BsaAI yAC'GTr Cuts at: 493 1204 3156 493 Size: 711 1952 2702 Fragments arranged by size: 2702 1952 711 BsaBI GATnn'nnATC Cuts at: 2372 5359 2372 Size: 2987 2378 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2079 2627 3172 Size: 53 83 186 936 477 69 548 545 Cuts at: 3172 3554 4189 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 548 545 477 382 186 83 69 53 BsaWI w'CCGG_w Cuts at: 3798 4196 4470 4617 5028 3798 Size: 398 274 147 411 4135 Fragments arranged by size: 4135 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 1178 Size: 30 165 30 1086 30 4024 Fragments arranged by size: 4024 1086 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2275 2494 1186 Size: 1089 219 4057 Fragments arranged by size: 4057 1089 219 BseYI C'CCAG_C Cuts at: 4568 5064 4568 Size: 496 4869 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4792 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 2815 3576 4604 1540 Size: 275 186 75 739 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 275 186 75 BsiHKAI G_wGCw'C Cuts at: 2103 2417 3426 3511 4582 2103 Size: 314 1009 85 1071 2886 Fragments arranged by size: 2886 1071 1009 314 85 BsmI GAATG_Cn' Cuts at: 2876 2969 2876 Size: 93 5272 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2400 2417 2562 2684 3426 3511 Size: 399 313 297 17 145 122 742 85 Cuts at: 3511 4582 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 313 297 145 122 85 17 BspEI T'CCGG_A Cuts at: 5028 5028 Size: 5365 BspHI T'CATG_A Cuts at: 3254 4984 3254 Size: 1730 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2720 844 Size: 1876 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3252 1394 Size: 429 1429 3507 Fragments arranged by size: 3507 1429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2128 2337 3860 4034 65 Size: 1183 880 209 1523 174 1396 Fragments arranged by size: 1523 1396 1183 880 209 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4009 4196 1700 Size: 110 2199 187 2869 Fragments arranged by size: 2869 2199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5365 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5365 BssSI C'ACGA_G Cuts at: 3425 4437 3425 Size: 1012 4353 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5365 BstBI TT'CG_AA Cuts at: 4169 4169 Size: 5365 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5365 BstYI r'GATC_y Cuts at: 1972 2208 2695 3062 3447 3464 4916 5002 5014 Size: 236 487 367 385 17 1452 86 12 Cuts at: 5014 5360 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 487 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2617 513 Size: 552 939 9 604 3261 Fragments arranged by size: 3261 939 604 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4626 Fragments arranged by size: 4626 683 56 BtsI GCAGTG_nn' Cuts at: 2298 2881 3675 3695 2298 Size: 583 794 20 3968 Fragments arranged by size: 3968 794 583 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5330 DraI TTT'AAA Cuts at: 2669 2669 Size: 5365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4372 809 Size: 507 3056 1802 Fragments arranged by size: 3056 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2812 3701 Size: 54 1425 50 275 18 243 739 889 Cuts at: 3701 5330 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 739 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2073 2812 1537 Size: 275 261 739 4090 Fragments arranged by size: 4090 739 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 3295 1353 Size: 1070 872 3423 Fragments arranged by size: 3423 1070 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3952 4326 4472 387 Size: 1064 138 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3428 4025 4812 5080 1239 Size: 393 1796 597 787 268 1524 Fragments arranged by size: 1796 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2246 2659 2246 Size: 413 4952 EcoRI G'AATT_C Cuts at: 2674 3052 2674 Size: 378 4987 FspI TGC'GCA Cuts at: 3871 3871 Size: 5365 HaeII r_GCGC'y Cuts at: 1678 2013 2039 2630 4512 1678 Size: 335 26 591 1882 2531 Fragments arranged by size: 2531 1882 591 335 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373 Size: 32 135 32 2434 32 42 32 2626 Fragments arranged by size: 2626 2434 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 2955 669 Size: 988 281 771 246 3079 Fragments arranged by size: 3079 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5365 HpaI GTT'AAC Cuts at: 2955 2955 Size: 5365 KasI G'GCGC_C Cuts at: 2009 2626 2009 Size: 617 4748 KpnI G_GTAC'C Cuts at: 2449 2617 2690 2449 Size: 168 73 5124 Fragments arranged by size: 5124 168 73 MfeI C'AATT_G Cuts at: 2964 2964 Size: 5365 MluI A'CGCG_T Cuts at: 5241 5241 Size: 5365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2296 4479 4663 1305 Size: 991 2183 184 2007 Fragments arranged by size: 2183 2007 991 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3543 Fragments arranged by size: 3543 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3323 3682 3841 518 Size: 648 2157 359 159 2042 Fragments arranged by size: 2157 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2088 2619 2781 3458 4606 4851 1606 Size: 409 73 531 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 409 245 162 73 NarI GG'CG_CC Cuts at: 2010 2627 2010 Size: 617 4748 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5365 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5365 NotI GC'GGCC_GC Cuts at: 2812 2812 Size: 5365 NspI r_CATG'y Cuts at: 1132 1229 2723 4268 1132 Size: 97 1494 1545 2229 Fragments arranged by size: 2229 1545 1494 97 PasI CC'CwG_GG Cuts at: 1348 2400 1348 Size: 1052 4313 PciI A'CATG_T Cuts at: 4264 4264 Size: 5365 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5365 PmeI GTTT'AAAC Cuts at: 2669 2669 Size: 5365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4947 4979 3434 Size: 32 1481 32 3820 Fragments arranged by size: 3820 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2659 2659 Size: 5365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5365 PsiI TTA'TAA Cuts at: 2935 2935 Size: 5365 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2429 2461 2429 Size: 32 5333 PstI C_TGCA'G Cuts at: 830 2087 2717 830 Size: 1257 630 3478 Fragments arranged by size: 3478 1257 630 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5365 PvuII CAG'CTG Cuts at: 2088 2088 Size: 5365 SacII CC_GC'GG Cuts at: 2016 2620 2016 Size: 604 4761 SalI G'TCGA_C Cuts at: 1936 2707 1936 Size: 771 4594 SapI GCTCTTCn'nnn_ Cuts at: 2423 2423 Size: 5365 SbfI CC_TGCA'GG Cuts at: 2717 2717 Size: 5365 ScaI AGT'ACT Cuts at: 3613 5325 3613 Size: 1712 3653 SfcI C'TryA_G Cuts at: 826 1045 2083 2713 3848 4529 4720 826 Size: 219 1038 630 1135 681 191 1471 Fragments arranged by size: 1471 1135 1038 681 630 219 191 SfoI GGC'GCC Cuts at: 2011 2628 2011 Size: 617 4748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5365 SmaI CCC'GGG Cuts at: 1872 2692 1872 Size: 820 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3469 4370 4632 820 Size: 197 926 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3156 493 Size: 2663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5365 SphI G_CATG'C Cuts at: 1132 2723 1132 Size: 1591 3774 SspI AAT'ATT Cuts at: 5 52 3289 5 Size: 47 3237 2081 Fragments arranged by size: 3237 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5365 StyI C'CwwG_G Cuts at: 513 1065 2004 2804 513 Size: 552 939 800 3074 Fragments arranged by size: 3074 939 800 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 3392 3409 3562 3747 4192 325 Size: 1255 855 957 17 153 185 445 1498 Fragments arranged by size: 1498 1255 957 855 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3611 5323 96 Size: 274 80 33 51 1443 1634 1712 138 Fragments arranged by size: 1712 1634 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5325 5357 5325 Size: 32 5333 Tth111I GACn'n_nGTC Cuts at: 1316 2534 1316 Size: 1218 4147 XbaI T'CTAG_A Cuts at: 2701 2701 Size: 5365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5365 XmaI C'CCGG_G Cuts at: 1870 2690 1870 Size: 820 4545 XmnI GAAnn'nnTTC Cuts at: 3494 3494 Size: 5365 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3173 276 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI