################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BanI BbeI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 412 GAATTT [GA]^AATT[CT] AgeI 360 ACCGGT A^CCGGT AhdI 298 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 412 GAATTT [GA]^AATT[CT] AspEI 298 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 313 GTGCTC G[AT]GC[AT]^C AvaII 522 GGTCC G^G[AT]CC BalI 128 TGGCCA TGG^CCA BamHI 201 GGATCC G^GATCC BbrPI 481 CACGTG CAC^GTG BcgI 408 CGAGGAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 95 CCCGG CC^[GC]GG BsaAI 481 CACGTG [CT]AC^GT[GA] BsaBI 21 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 360 ACCGGT [AT]^CCGG[AT] BseRI 49 GAGGAG GAGGAG(10/8) BsiHKAI 313 GTGCTC G[AT]GC[AT]^C BsmAI 436 GAGAC GTCTC(1/5) BsmBI 436 GAGACG CGTCTC(1/5) BsmFI 523 GTCCC GGGAC(10/14) BstF5I 132 CATCC GGATG(2/0) CfoI 33 GCGC GCG^C DdeI 434 CTGAG C^T[ACGT]AG DraIII 349 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 298 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 558 CTCTTC CTCTTC(1/4) FokI 132 CATCC GGATG(9/13) HgaI 438 GACGC GACGC(5/10) HhaI 33 GCGC GCG^C HinP1I 33 GCGC G^CGC HinfI 251 GAGTC G^A[ACGT]TC HphI 138 GGTGA GGTGA(8/7) Ksp632I 558 CTCTTC CTCTTC(1/4) MamI 21 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 559 TCTTC GAAGA(8/7) MluNI 128 TGGCCA TGG^CCA MscI 128 TGGCCA TGG^CCA NciI 95 CCCGG CC^[GC]GG PinAI 360 ACCGGT A^CCGGT PleI 251 GAGTC GAGTC(4/5) PmaCI 481 CACGTG CAC^GTG PmlI 481 CACGTG CAC^GTG PpuMI 521 AGGTCCC [GA]G^G[AT]CC[CT] ScaI 173 AGTACT AGT^ACT SfcI 474 CTACAG C^T[GA][CT]AG SmlI 343 CTCAAG C^T[CT][GA]AG TaqI 29 TCGA T^CGA Tsp509I 413 AATT ^AATT TthHB8I 29 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 174 GTAC GT^AC 366 GTAC ApaI 92 GGGCCC GGGCC^C 260 GGGCCC BpmI 208 CTGGAG CTGGAG(16/14) 394 CTGGAG BsgI 79 GTGCAG GTGCAG(16/14) 514 GTGCAG BspMI 284 ACCTGC ACCTGC(4/8) 519 GCAGGT BsrBI 40 GAGCGG CCGCTC(-3/-3) 217 GAGCGG BsrFI 360 ACCGGT [GA]^CCGG[CT] 543 ACCGGC BsrI 183 CCAGT ACTGG(1/-1) 419 CCAGT BstYI 201 GGATCC [GA]^GATC[CT] 374 AGATCC Cfr10I 360 ACCGGT [GA]^CCGG[CT] 543 ACCGGC MaeII 119 ACGT A^CGT 482 ACGT MaeIII 422 GTGAC ^GT[ACGT]AC 484 GTGAC MflI 201 GGATCC [GA]^GATC[CT] 374 AGATCC MslI 132 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 270 CACTGTCGTG PvuII 108 CAGCTG CAG^CTG 229 CAGCTG RsaI 174 GTAC GT^AC 366 GTAC Tsp45I 422 GTGAC ^GT[GC]AC 484 GTGAC XhoII 201 GGATCC [GA]^GATC[CT] 374 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboI MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI