(Circular) (Six-base) MAPSORT of: pFN21AE6274.seq Check: 8082 from: 1 to: 5275 pFN21AE6274 5275 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3085 278 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2618 1937 Size: 681 4594 Acc65I G'GTAC_C Cuts at: 2596 2596 Size: 5275 AclI AA'CG_TT Cuts at: 1563 3402 3775 1563 Size: 1839 373 3063 Fragments arranged by size: 3063 1839 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 3338 4722 4990 1632 Size: 535 1171 1384 268 1917 Fragments arranged by size: 1917 1384 1171 535 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5078 AflIII A'CryG_T Cuts at: 1203 4174 5151 1203 Size: 2971 977 1327 Fragments arranged by size: 2971 1327 977 AgeI A'CCGG_T Cuts at: 2365 4106 2365 Size: 1741 3534 AhdI GACnn_n'nnGTC Cuts at: 2308 4004 2308 Size: 1696 3579 AleI CACnn'nnGTG Cuts at: 1166 2279 1166 Size: 1113 4162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5275 AlwNI CAG_nnn'CTG Cuts at: 3018 4590 3018 Size: 1572 3703 ApaI G_GGCC'C Cuts at: 2101 2269 2101 Size: 168 5107 ApaLI G'TGCA_C Cuts at: 3332 4488 3332 Size: 1156 4119 ApoI r'AATT_y Cuts at: 1493 2417 2584 2810 2962 5071 1493 Size: 924 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 3829 160 Size: 872 2797 1606 Fragments arranged by size: 2797 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 AvaI C'yCGr_G Cuts at: 1870 1943 2600 1870 Size: 73 657 4545 Fragments arranged by size: 4545 657 73 BamHI G'GATC_C Cuts at: 2206 2605 2972 2206 Size: 399 367 4509 Fragments arranged by size: 4509 399 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2596 4051 618 Size: 325 217 627 809 1455 1842 Fragments arranged by size: 1842 1455 809 627 325 217 BanII G_rGCy'C Cuts at: 1391 2101 2231 2269 2594 1391 Size: 710 130 38 325 4072 Fragments arranged by size: 4072 710 325 130 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2402 2436 3466 3500 1903 Size: 34 465 34 1030 34 3678 Fragments arranged by size: 3678 1030 465 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3167 4377 3167 Size: 1210 4065 BclI T'GATC_A Cuts at: 1548 1548 Size: 5275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3886 136 Size: 107 122 71 631 605 2214 1525 Fragments arranged by size: 2214 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5270 5270 Size: 5275 BlpI GC'TnA_GC Cuts at: 2692 2692 Size: 5275 Bme1580I G_kGCm'C Cuts at: 1790 2101 2269 3336 4492 1790 Size: 311 168 1067 1156 2573 Fragments arranged by size: 2573 1156 1067 311 168 BmgBI CAC'GTC Cuts at: 1318 2125 1318 Size: 807 4468 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3006 3964 442 Size: 416 53 83 399 24 1589 958 1753 Fragments arranged by size: 1753 1589 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5275 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2420 3935 1239 Size: 995 186 1515 2579 Fragments arranged by size: 2579 1515 995 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3400 4265 4563 2333 Size: 1067 865 298 3045 Fragments arranged by size: 3045 1067 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3938 882 Size: 617 2439 2219 Fragments arranged by size: 2439 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2488 3066 493 Size: 711 1284 578 2702 Fragments arranged by size: 2702 1284 711 578 BsaBI GATnn'nnATC Cuts at: 2030 5269 2030 Size: 3239 2036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3082 3464 4099 275 Size: 53 83 186 936 1549 382 635 1451 Fragments arranged by size: 1549 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2365 3708 4106 4380 4527 4938 2365 Size: 1343 398 274 147 411 2702 Fragments arranged by size: 2702 1343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5050 Fragments arranged by size: 5050 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 1186 Size: 883 4392 BseYI C'CCAG_C Cuts at: 2232 2514 4478 4974 2232 Size: 282 1964 496 2533 Fragments arranged by size: 2533 1964 496 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 2540 1163 Size: 573 369 435 3898 Fragments arranged by size: 3898 573 435 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 3486 4514 1540 Size: 275 186 724 761 1028 2301 Fragments arranged by size: 2301 1028 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 3336 3421 4492 2322 Size: 1014 85 1071 3105 Fragments arranged by size: 3105 1071 1014 85 BsmI GAATG_Cn' Cuts at: 2786 2879 2786 Size: 93 5182 BsmBI CGTCTCn'nnnn_ Cuts at: 2435 2435 Size: 5275 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2231 2269 2322 2594 3336 3421 Size: 399 311 130 38 53 272 742 85 Cuts at: 3421 4492 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 311 272 130 85 53 38 BspEI T'CCGG_A Cuts at: 4938 4938 Size: 5275 BspHI T'CATG_A Cuts at: 3164 4894 3164 Size: 1730 3545 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 2515 2630 844 Size: 1454 217 115 3489 Fragments arranged by size: 3489 1454 217 115 BsrBI CCG'CTC Cuts at: 1394 1823 2047 2224 3162 1394 Size: 429 224 177 938 3507 Fragments arranged by size: 3507 938 429 224 177 BsrDI GCAATG_nn' Cuts at: 65 1248 3770 3944 65 Size: 1183 2522 174 1396 Fragments arranged by size: 2522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2365 2548 2572 3919 4106 1700 Size: 110 555 183 24 1347 187 2869 Fragments arranged by size: 2869 1347 555 187 183 110 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5275 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5275 BssSI C'ACGA_G Cuts at: 3335 4347 3335 Size: 1012 4263 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5275 BstBI TT'CG_AA Cuts at: 4079 4079 Size: 5275 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5275 BstYI r'GATC_y Cuts at: 1972 2206 2379 2605 2972 3357 3374 4826 4912 Size: 234 173 226 367 385 17 1452 86 Cuts at: 4912 4924 5270 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 234 226 173 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3784 Fragments arranged by size: 3784 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4536 Fragments arranged by size: 4536 683 56 BtsI GCAGTG_nn' Cuts at: 2555 2791 3585 3605 2555 Size: 236 794 20 4225 Fragments arranged by size: 4225 794 236 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5275 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2449 2484 529 Size: 35 1885 35 3320 Fragments arranged by size: 3320 1885 35 35 DraI TTT'AAA Cuts at: 2579 2579 Size: 5275 DraIII CAC_nnn'GTG Cuts at: 2359 2359 Size: 5275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4282 809 Size: 507 2966 1802 Fragments arranged by size: 2966 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2169 2550 Size: 54 1425 50 275 18 303 36 381 Cuts at: 2550 2722 3611 5240 8 Size: 172 889 1629 43 Fragments arranged by size: 1629 1425 889 381 303 275 172 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2722 1537 Size: 275 910 4090 Fragments arranged by size: 4090 910 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2569 3205 1353 Size: 1216 636 3423 Fragments arranged by size: 3423 1216 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 3862 4236 4382 387 Size: 1064 138 495 1778 374 146 1280 Fragments arranged by size: 1778 1280 1064 495 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2234 2420 3338 3935 4722 4990 Size: 393 535 67 186 918 597 787 268 Cuts at: 4990 1239 Size: 1524 Fragments arranged by size: 1524 918 787 597 535 393 268 186 67 EcoO109I rG'GnC_Cy Cuts at: 2265 2266 2527 2265 Size: 1 261 5013 Fragments arranged by size: 5013 261 1 EcoRI G'AATT_C Cuts at: 2584 2962 2584 Size: 378 4897 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 5275 FspI TGC'GCA Cuts at: 3781 3781 Size: 5275 HaeII r_GCGC'y Cuts at: 1678 4422 1678 Size: 2744 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2294 2326 3916 Size: 32 135 32 473 32 217 32 1590 Cuts at: 3916 3948 3990 4022 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1590 473 217 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2619 2865 669 Size: 988 281 681 246 3079 Fragments arranged by size: 3079 988 681 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5275 HpaI GTT'AAC Cuts at: 2865 2865 Size: 5275 KpnI G_GTAC'C Cuts at: 2600 2600 Size: 5275 MfeI C'AATT_G Cuts at: 2874 2874 Size: 5275 MluI A'CGCG_T Cuts at: 5151 5151 Size: 5275 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4389 4573 1305 Size: 3084 184 2007 Fragments arranged by size: 3084 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 10 Size: 54 1425 343 303 3150 Fragments arranged by size: 3150 1425 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2279 3233 3592 3751 518 Size: 648 975 138 954 359 159 2042 Fragments arranged by size: 2042 975 954 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2115 2236 2495 2691 3368 4516 4761 1606 Size: 509 121 259 196 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 509 259 245 196 121 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3784 Fragments arranged by size: 3784 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5275 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5275 NotI GC'GGCC_GC Cuts at: 2722 2722 Size: 5275 NspI r_CATG'y Cuts at: 1132 1229 2633 4178 1132 Size: 97 1404 1545 2229 Fragments arranged by size: 2229 1545 1404 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5275 PciI A'CATG_T Cuts at: 4174 4174 Size: 5275 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5275 PmeI GTTT'AAAC Cuts at: 2579 2579 Size: 5275 PmlI CAC'GTG Cuts at: 2488 2488 Size: 5275 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2150 2182 3344 3376 4857 4889 2150 Size: 32 1162 32 1481 32 2536 Fragments arranged by size: 2536 1481 1162 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2527 Size: 5275 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5275 PsiI TTA'TAA Cuts at: 2845 2845 Size: 5275 PspOMI G'GGCC_C Cuts at: 2097 2265 2097 Size: 168 5107 PstI C_TGCA'G Cuts at: 830 2627 830 Size: 1797 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5275 PvuII CAG'CTG Cuts at: 2115 2236 2115 Size: 121 5154 SalI G'TCGA_C Cuts at: 1936 2617 1936 Size: 681 4594 SbfI CC_TGCA'GG Cuts at: 2627 2627 Size: 5275 ScaI AGT'ACT Cuts at: 2180 3523 5235 2180 Size: 1343 1712 2220 Fragments arranged by size: 2220 1712 1343 SfcI C'TryA_G Cuts at: 826 1045 2479 2623 3758 4439 4630 826 Size: 219 1434 144 1135 681 191 1471 Fragments arranged by size: 1471 1434 1135 681 219 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5275 SmaI CCC'GGG Cuts at: 1872 2602 1872 Size: 730 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 3379 4280 4542 820 Size: 197 926 405 1031 901 262 1553 Fragments arranged by size: 1553 1031 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 3066 493 Size: 2573 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5275 SphI G_CATG'C Cuts at: 1132 2633 1132 Size: 1501 3774 SspI AAT'ATT Cuts at: 5 52 3199 5 Size: 47 3147 2081 Fragments arranged by size: 3147 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5275 StyI C'CwwG_G Cuts at: 513 1065 2004 2714 513 Size: 552 939 710 3074 Fragments arranged by size: 3074 939 710 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2506 3302 3319 3472 3657 4102 325 Size: 1255 926 796 17 153 185 445 1498 Fragments arranged by size: 1498 1255 926 796 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 2370 3521 Size: 274 80 33 51 1443 201 192 1151 Cuts at: 3521 5233 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1151 274 201 192 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5235 5267 5235 Size: 32 5243 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5275 XbaI T'CTAG_A Cuts at: 2611 2611 Size: 5275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5275 XmaI C'CCGG_G Cuts at: 1870 2600 1870 Size: 730 4545 XmnI GAAnn'nnTTC Cuts at: 3404 3404 Size: 5275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3083 276 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI