(Linear) (Six-base) MAPSORT of: KIEE6274.seq Check: 1027 from: 1 to: 570 KIEE6274.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 162 570 Size: 162 408 AgeI A'CCGG_T Cuts at: 0 360 570 Size: 360 210 AhdI GACnn_n'nnGTC Cuts at: 0 303 570 Size: 303 267 AleI CACnn'nnGTG Cuts at: 0 274 570 Size: 274 296 ApaI G_GGCC'C Cuts at: 0 96 264 570 Size: 96 168 306 Fragments arranged by size: 306 168 96 ApoI r'AATT_y Cuts at: 0 412 570 Size: 412 158 BamHI G'GATC_C Cuts at: 0 201 570 Size: 201 369 BanII G_rGCy'C Cuts at: 0 96 226 264 570 Size: 96 130 38 306 Fragments arranged by size: 306 130 96 38 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 397 431 570 Size: 397 34 139 Fragments arranged by size: 397 139 34 Bme1580I G_kGCm'C Cuts at: 0 96 264 570 Size: 96 168 306 Fragments arranged by size: 306 168 96 BmgBI CAC'GTC Cuts at: 0 120 570 Size: 120 450 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 415 570 Size: 229 186 155 Fragments arranged by size: 229 186 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 570 Size: 328 242 BsaAI yAC'GTr Cuts at: 0 483 570 Size: 483 87 BsaBI GATnn'nnATC Cuts at: 0 25 570 Size: 25 545 BsaWI w'CCGG_w Cuts at: 0 360 570 Size: 360 210 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 64 570 Size: 64 506 BseYI C'CCAG_C Cuts at: 0 227 509 570 Size: 227 282 61 Fragments arranged by size: 282 227 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 535 570 Size: 100 435 35 Fragments arranged by size: 435 100 35 BsiHKAI G_wGCw'C Cuts at: 0 317 570 Size: 317 253 BsmBI CGTCTCn'nnnn_ Cuts at: 0 430 570 Size: 430 140 Bsp1286I G_dGCh'C Cuts at: 0 96 226 264 317 570 Size: 96 130 38 53 253 Fragments arranged by size: 253 130 96 53 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 293 510 570 Size: 293 217 60 Fragments arranged by size: 293 217 60 BsrBI CCG'CTC Cuts at: 0 42 219 570 Size: 42 177 351 Fragments arranged by size: 351 177 42 BsrFI r'CCGG_y Cuts at: 0 360 543 570 Size: 360 183 27 Fragments arranged by size: 360 183 27 BstYI r'GATC_y Cuts at: 0 201 374 570 Size: 201 173 196 Fragments arranged by size: 201 196 173 BtsI GCAGTG_nn' Cuts at: 0 550 570 Size: 550 20 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 444 479 570 Size: 444 35 91 Fragments arranged by size: 444 91 35 DraIII CAC_nnn'GTG Cuts at: 0 354 570 Size: 354 216 EaeI y'GGCC_r Cuts at: 0 128 164 545 570 Size: 128 36 381 25 Fragments arranged by size: 381 128 36 25 EarI CTCTTCn'nnn_ Cuts at: 0 564 570 Size: 564 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 79 570 Size: 79 491 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 162 229 415 570 Size: 162 67 186 155 Fragments arranged by size: 186 162 155 67 EcoO109I rG'GnC_Cy Cuts at: 0 260 261 522 570 Size: 260 1 261 48 Fragments arranged by size: 261 260 48 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 192 570 Size: 192 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 40 72 289 321 570 Size: 40 32 217 32 249 Fragments arranged by size: 249 217 40 32 32 MscI TGG'CCA Cuts at: 0 130 570 Size: 130 440 MslI CAynn'nnrTG Cuts at: 0 136 274 570 Size: 136 138 296 Fragments arranged by size: 296 138 136 MspA1I CmG'CkG Cuts at: 0 110 231 490 570 Size: 110 121 259 80 Fragments arranged by size: 259 121 110 80 PmlI CAC'GTG Cuts at: 0 483 570 Size: 483 87 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 145 177 570 Size: 145 32 393 Fragments arranged by size: 393 145 32 PpuMI rG'GwC_Cy Cuts at: 0 522 570 Size: 522 48 PspOMI G'GGCC_C Cuts at: 0 92 260 570 Size: 92 168 310 Fragments arranged by size: 310 168 92 PvuII CAG'CTG Cuts at: 0 110 231 570 Size: 110 121 339 Fragments arranged by size: 339 121 110 ScaI AGT'ACT Cuts at: 0 175 570 Size: 175 395 SfcI C'TryA_G Cuts at: 0 474 570 Size: 474 96 SmlI C'TyrA_G Cuts at: 0 343 570 Size: 343 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 501 570 Size: 501 69 TatI w'GTAC_w Cuts at: 0 173 365 570 Size: 173 192 205 Fragments arranged by size: 205 192 173 Enzymes that do cut: AcuI AgeI AhdI AleI ApaI ApoI BamHI BanII BcgI Bme1580I BmgBI BpmI BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstYI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I PmlI PpiI PpuMI PspOMI PvuII ScaI SfcI SmlI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI