(Circular) (Six-base) MAPSORT of: pFN21AE3196.seq Check: 5905 from: 1 to: 6115 pFN21AE3196 6115 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2938 2938 Size: 6115 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3458 1937 Size: 1521 4594 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 4178 5562 5830 1632 Size: 572 1974 1384 268 1917 Fragments arranged by size: 1974 1917 1384 572 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2369 2699 2870 1184 Size: 1185 330 171 4429 Fragments arranged by size: 4429 1185 330 171 AlwNI CAG_nnn'CTG Cuts at: 2597 3858 5430 2597 Size: 1261 1572 3282 Fragments arranged by size: 3282 1572 1261 ApaI G_GGCC'C Cuts at: 2960 2960 Size: 6115 ApaLI G'TGCA_C Cuts at: 3323 4172 5328 3323 Size: 849 1156 4110 Fragments arranged by size: 4110 1156 849 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2283 3440 1870 Size: 73 119 221 1157 4545 Fragments arranged by size: 4545 1157 221 119 73 BamHI G'GATC_C Cuts at: 2266 3445 3812 2266 Size: 1179 367 4569 Fragments arranged by size: 4569 1179 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2846 3016 3436 4891 618 Size: 325 217 627 1059 170 420 1455 1842 Fragments arranged by size: 1842 1455 1059 627 420 325 217 170 BanII G_rGCy'C Cuts at: 1391 2136 2535 2960 3255 3366 3411 3434 1391 Size: 745 399 425 295 111 45 23 4072 Fragments arranged by size: 4072 745 425 399 295 111 45 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2664 928 Size: 971 765 4379 Fragments arranged by size: 4379 971 765 BbvCI CC'TCA_GC Cuts at: 2391 2391 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4306 4340 1903 Size: 34 2369 34 3678 Fragments arranged by size: 3678 2369 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BfrBI ATG'CAT Cuts at: 3118 3118 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2286 4726 136 Size: 107 122 71 631 605 614 2440 1525 Fragments arranged by size: 2440 1525 631 614 605 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 2762 3532 2762 Size: 770 5345 Bme1580I G_kGCm'C Cuts at: 1790 2849 2960 3000 3021 3231 3327 4176 5332 Size: 1059 111 40 21 210 96 849 1156 Cuts at: 5332 1790 Size: 2573 Fragments arranged by size: 2573 1156 1059 849 210 111 96 40 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2560 3846 4804 Size: 416 53 83 399 24 1143 1286 958 Cuts at: 4804 442 Size: 1753 Fragments arranged by size: 1753 1286 1143 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2520 3005 4775 1239 Size: 1281 485 1770 2579 Fragments arranged by size: 2579 1770 1281 485 Bpu10I CC'TnA_GC Cuts at: 2391 2391 Size: 6115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1998 4240 5105 5403 1998 Size: 2242 865 298 2710 Fragments arranged by size: 2710 2242 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2549 4778 882 Size: 617 1050 2229 2219 Fragments arranged by size: 2229 2219 1050 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3130 3906 493 Size: 711 1170 756 776 2702 Fragments arranged by size: 2702 1170 776 756 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2154 2818 3111 Size: 53 83 186 936 558 63 664 293 Cuts at: 3111 3922 4304 4939 275 Size: 811 382 635 1451 Fragments arranged by size: 1451 936 811 664 635 558 382 293 186 83 63 53 BsaWI w'CCGG_w Cuts at: 2364 4548 4946 5220 5367 5778 2364 Size: 2184 398 274 147 411 2701 Fragments arranged by size: 2701 2184 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5890 Fragments arranged by size: 5890 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 3002 1186 Size: 1291 525 4299 Fragments arranged by size: 4299 1291 525 BseYI C'CCAG_C Cuts at: 2467 2475 2587 3371 3384 5318 5814 2467 Size: 8 112 784 13 1934 496 2768 Fragments arranged by size: 2768 1934 784 496 112 13 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 2659 2711 3038 3069 3123 3332 Size: 573 660 263 52 327 31 54 209 Cuts at: 3332 1163 Size: 3946 Fragments arranged by size: 3946 660 573 327 263 209 54 52 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2459 2887 3565 4326 5354 1540 Size: 275 186 458 428 678 761 1028 2301 Fragments arranged by size: 2301 1028 761 678 458 428 275 186 BsiHKAI G_wGCw'C Cuts at: 2101 2295 2763 3327 4176 4261 5332 2101 Size: 194 468 564 849 85 1071 2884 Fragments arranged by size: 2884 1071 849 564 468 194 85 BsmI GAATG_Cn' Cuts at: 3212 3626 3719 3212 Size: 414 93 5608 Fragments arranged by size: 5608 414 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2496 2496 Size: 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2136 2295 2535 2763 2849 2960 Size: 399 311 35 159 240 228 86 111 Cuts at: 2960 3000 3021 3231 3255 3327 3366 3411 3434 Size: 40 21 210 24 72 39 45 23 Cuts at: 3434 4176 4261 5332 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 311 240 228 210 159 111 86 85 72 45 40 39 35 24 23 21 BspEI T'CCGG_A Cuts at: 2364 5778 2364 Size: 3414 2701 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2635 2724 2938 3470 844 Size: 1791 89 214 532 3489 Fragments arranged by size: 3489 1791 532 214 89 BsrBI CCG'CTC Cuts at: 1394 1823 2012 2428 2542 4002 1394 Size: 429 189 416 114 1460 3507 Fragments arranged by size: 3507 1460 429 416 189 114 BsrDI GCAATG_nn' Cuts at: 65 1248 2781 4610 4784 65 Size: 1183 1533 1829 174 1396 Fragments arranged by size: 1829 1533 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 2142 2694 4759 4946 1700 Size: 110 283 49 552 2065 187 2869 Fragments arranged by size: 2869 2065 552 283 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 2516 3147 4175 5187 2516 Size: 631 1028 1012 3444 Fragments arranged by size: 3444 1028 1012 631 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6115 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 2647 1184 Size: 1463 4652 BstXI CCAn_nnnn'nTGG Cuts at: 2633 2633 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2266 3445 3812 4197 4214 5666 5752 5764 Size: 294 1179 367 385 17 1452 86 12 Cuts at: 5764 6110 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1179 385 367 346 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2109 2202 2249 2297 2881 3090 3177 Size: 552 1044 93 47 48 584 209 87 Cuts at: 3177 3231 513 Size: 54 3397 Fragments arranged by size: 3397 1044 584 552 209 93 87 54 48 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 3047 3076 3285 3631 4425 4445 3047 Size: 29 209 346 794 20 4717 Fragments arranged by size: 4717 794 346 209 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DraIII CAC_nnn'GTG Cuts at: 2521 2977 2521 Size: 456 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 3174 3562 Size: 54 1425 50 275 18 1054 290 388 Cuts at: 3562 4451 6080 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1054 889 388 290 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 3562 1537 Size: 275 1072 678 4090 Fragments arranged by size: 4090 1072 678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2366 2756 2905 3173 3399 4045 1353 Size: 1013 390 149 268 226 646 3423 Fragments arranged by size: 3423 1013 646 390 268 226 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2446 4702 5076 5222 387 Size: 1064 138 857 2256 374 146 1280 Fragments arranged by size: 2256 1280 1064 857 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2520 3005 4178 4775 5562 5830 Size: 393 572 316 485 1173 597 787 268 Cuts at: 5830 1239 Size: 1524 Fragments arranged by size: 1524 1173 787 597 572 485 393 316 268 EcoO109I rG'GnC_Cy Cuts at: 2229 2956 2229 Size: 727 5388 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FspI TGC'GCA Cuts at: 2873 4621 2873 Size: 1748 4367 HaeII r_GCGC'y Cuts at: 1678 2214 2225 5262 1678 Size: 536 11 3037 2531 Fragments arranged by size: 3037 2531 536 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4756 4788 4830 4862 1373 Size: 32 135 32 3184 32 42 32 2626 Fragments arranged by size: 3184 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3459 3705 669 Size: 988 281 1521 246 3079 Fragments arranged by size: 3079 1521 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6115 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 5229 5413 1305 Size: 1134 2790 184 2007 Fragments arranged by size: 2790 2007 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3176 10 Size: 54 1425 343 1344 2949 Fragments arranged by size: 2949 1425 1344 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 4073 4432 4591 518 Size: 648 1248 1659 359 159 2042 Fragments arranged by size: 2042 1659 1248 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2242 2251 2273 2299 2475 2883 3099 Size: 431 205 9 22 26 176 408 216 Cuts at: 3099 3107 3351 3375 3531 4208 5356 5601 1606 Size: 8 244 24 156 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 431 408 245 244 216 205 176 156 26 24 22 9 8 NaeI GCC'GGC Cuts at: 2144 2696 2144 Size: 552 5563 NcoI C'CATG_G Cuts at: 513 1065 3090 3177 513 Size: 552 2025 87 3451 Fragments arranged by size: 3451 2025 552 87 NdeI CA'TA_TG Cuts at: 387 3195 387 Size: 2808 3307 NgoMIV G'CCGG_C Cuts at: 2142 2694 2142 Size: 552 5563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NsiI A_TGCA'T Cuts at: 3120 3120 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 3473 5018 1132 Size: 97 2244 1545 2229 Fragments arranged by size: 2244 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6115 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6115 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PmlI CAC'GTG Cuts at: 2374 2374 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2229 2229 Size: 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 2956 2956 Size: 6115 PstI C_TGCA'G Cuts at: 830 2107 3467 830 Size: 1277 1360 3478 Fragments arranged by size: 3478 1360 1277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 2037 3107 3375 2037 Size: 1070 268 4777 Fragments arranged by size: 4777 1070 268 SacII CC_GC'GG Cuts at: 2252 2300 2884 2252 Size: 48 584 5483 Fragments arranged by size: 5483 584 48 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SanDI GG'GwC_CC Cuts at: 2229 2229 Size: 6115 SapI GCTCTTCn'nnn_ Cuts at: 2366 3399 2366 Size: 1033 5082 SbfI CC_TGCA'GG Cuts at: 3467 3467 Size: 6115 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2103 2904 3463 4598 5279 5470 826 Size: 219 1058 801 559 1135 681 191 1471 Fragments arranged by size: 1471 1135 1058 801 681 559 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 2093 1700 Size: 393 5722 SmaI CCC'GGG Cuts at: 1872 2285 3442 1872 Size: 413 1157 4545 Fragments arranged by size: 4545 1157 413 SmlI C'TyrA_G Cuts at: 820 1017 1943 2013 4219 5120 5382 820 Size: 197 926 70 2206 901 262 1553 Fragments arranged by size: 2206 1553 926 901 262 197 70 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 3473 1132 Size: 2341 3774 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 2351 1517 Size: 834 5281 StyI C'CwwG_G Cuts at: 513 1065 3090 3177 3255 3554 513 Size: 552 2025 87 78 299 3074 Fragments arranged by size: 3074 2025 552 299 87 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2722 3103 3342 4142 4159 4312 4497 Size: 1255 1142 381 239 800 17 153 185 Cuts at: 4497 4942 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1142 800 445 381 239 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4361 6073 96 Size: 274 80 33 51 1443 2384 1712 138 Fragments arranged by size: 2384 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2232 3336 2232 Size: 1104 5011 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 2283 3440 1870 Size: 413 1157 4545 Fragments arranged by size: 4545 1157 413 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI