################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I Bst1107I BstBI BstZ17I ClaI DraI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspI NspV PacI PflFI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tru9I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AfaI 1422 GTAC GT^AC AgeI 939 ACCGGT A^CCGGT Alw44I 348 GTGCAC G^TGCAC AocI 1373 CCTCAGG CC^T[ACGT]AGG ApaLI 348 GTGCAC G^TGCAC AviII 485 TGCGCA TGC^GCA BalI 611 TGGCCA TGG^CCA BbeI 169 GGCGCC GGCGC^C BbvCI 195 CCTCAGC CCTCAGC(-5/-2) BcgI 981 GCAGAATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 478 CTAG C^TAG BsaI 537 GGTCTC GGTCTC(1/5) BsaWI 939 ACCGGT [AT]^CCGG[AT] BsiEI 298 CGGCCG CG[GA][CT]^CG Bsp1407I 1421 TGTACA T^GTACA BsrBI 376 GAGCGG CCGCTC(-3/-3) BsrDI 1119 GCAATG GCAATG(2/0) BsrGI 1421 TGTACA T^GTACA BssSI 373 CACGAG CACGAG(-5/-1) Bsu36I 1373 CCTCAGG CC^T[ACGT]AGG CpoI 319 CGGACCG CG^G[AT]CCG DraIII 1177 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 298 CGGCCG C^GGCCG EclXI 298 CGGCCG C^GGCCG Eco52I 298 CGGCCG C^GGCCG Eco81I 1373 CCTCAGG CC^T[ACGT]AGG EcoT14I 687 CCATGG C^C[AT][AT]GG FspI 485 TGCGCA TGC^GCA HinfI 745 GAATC G^A[ACGT]TC KasI 169 GGCGCC G^GCGCC MaeI 478 CTAG C^TAG MaeII 188 ACGT A^CGT MluNI 611 TGGCCA TGG^CCA MscI 611 TGGCCA TGG^CCA MslI 603 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 842 GCCGGC GCC^GGC NarI 169 GGCGCC GG^CGCC NcoI 687 CCATGG C^CATGG NgoMIV 842 GCCGGC G^CCGGC NotI 297 GCGGCCGC GC^GGCCGC PflMI 810 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 939 ACCGGT A^CCGGT RsaI 1422 GTAC GT^AC RsrII 319 CGGACCG CG^G[AT]CCG SapI 309 GCTCTTC GCTCTTC(1/4) SbfI 738 CCTGCAGG CCTGCA^GG SfoI 169 GGCGCC GGC^GCC Sse8387I 738 CCTGCAGG CCTGCA^GG SspBI 1421 TGTACA T^GTACA StuI 1298 AGGCCT AGG^CCT StyI 687 CCATGG C^C[AT][AT]GG TfiI 745 GAATC G^A[AT]TC Tsp509I 985 AATT ^AATT Van91I 810 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 169 GGCGCC G[GA]^CG[CT]C 1192 GGCGTC BamHI 237 GGATCC G^GATCC 698 GGATCC BbsI 805 GAAGAC GAAGAC(2/6) 1462 GTCTTC BlpI 837 GCTAAGC GC^T[ACGT]AGC 1545 GCTCAGC Bpu1102I 837 GCTAAGC GC^T[ACGT]AGC 1545 GCTCAGC BpuAI 805 GAAGAC GAAGAC(2/6) 1462 GTCTTC BsaHI 169 GGCGCC G[GA]^CG[CT]C 1192 GGCGTC BsgI 339 GTGCAG GTGCAG(16/14) 1218 GTGCAG BsmBI 1194 CGTCTC CGTCTC(1/5) 641 GAGACG BsrFI 842 GCCGGC [GA]^CCGG[CT] 939 ACCGGT BstAPI 341 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 651 GCAGCGGCTGC BstEII 438 GGTTACC G^GT[ACGT]ACC 820 GGTGACC BstPI 438 GGTTACC G^GT[ACGT]ACC 820 GGTGACC BstXI 38 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 893 CCAAGCCTGTGG CelII 837 GCTAAGC GC^T[ACGT]AGC 1545 GCTCAGC Cfr10I 842 GCCGGC [GA]^CCGG[CT] 939 ACCGGT EcoNI 558 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 711 CCTGGAGCAGG EcoO65I 438 GGTTACC G^GT[ACGT]ACC 820 GGTGACC Hin1I 169 GGCGCC G[GA]^CG[CT]C 1192 GGCGTC PaeR7I 638 CTCGAG C^TCGAG 1474 CTCGAG SfiI 453 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 770 GGCCTCTTGGGCC SmaI 233 CCCGGG CCC^GGG 1076 CCCGGG XhoI 638 CTCGAG C^TCGAG 1474 CTCGAG XmaCI 233 CCCGGG C^CCGGG 1076 CCCGGG XmaI 233 CCCGGG C^CCGGG 1076 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I TspRI TthHB8I XhoII