(Circular) (Six-base) MAPSORT of: pFN21AE4126.seq Check: 3959 from: 1 to: 6256 pFN21AE4126 6256 bp With 182 enzymes: SgfI * March 28, 2012 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2713 3049 3109 2713 Size: 336 60 5860 Fragments arranged by size: 5860 336 60 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3599 1937 Size: 1662 4594 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2852 4319 5703 5971 1632 Size: 1220 1467 1384 268 1917 Fragments arranged by size: 1917 1467 1384 1220 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 2944 5087 2944 Size: 2143 4113 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2020 2575 2632 2662 3424 3518 3999 5571 2020 Size: 555 57 30 762 94 481 1572 2705 Fragments arranged by size: 2705 1572 762 555 481 94 57 30 ApaI G_GGCC'C Cuts at: 2235 3089 3099 3237 3537 2235 Size: 854 10 138 300 4954 Fragments arranged by size: 4954 854 300 138 10 ApaLI G'TGCA_C Cuts at: 2353 4313 5469 2353 Size: 1960 1156 3140 Fragments arranged by size: 3140 1960 1156 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2147 2238 2643 3081 3479 3581 1870 Size: 73 204 91 405 438 398 102 4545 Fragments arranged by size: 4545 438 405 398 204 102 91 73 BamHI G'GATC_C Cuts at: 2242 2703 3586 3953 2242 Size: 461 883 367 4545 Fragments arranged by size: 4545 883 461 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2890 2941 3150 3577 Size: 325 217 627 387 716 51 209 427 Cuts at: 3577 5032 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 716 627 427 387 325 217 209 51 BanII G_rGCy'C Cuts at: 1391 2058 2163 2235 2316 2715 3089 3099 3114 Size: 667 105 72 81 399 374 10 15 Cuts at: 3114 3237 3279 3411 3537 3575 1391 Size: 123 42 132 126 38 4072 Fragments arranged by size: 4072 667 399 374 132 126 123 105 81 72 42 38 15 10 BbeI G_GCGC'C Cuts at: 2178 2178 Size: 6256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2817 3460 928 Size: 971 918 643 3724 Fragments arranged by size: 3724 971 918 643 BbvCI CC'TCA_GC Cuts at: 2201 2201 Size: 6256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2975 3009 4447 4481 1903 Size: 34 1038 34 1438 34 3678 Fragments arranged by size: 3678 1438 1038 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2465 2773 2782 Size: 107 122 71 631 605 793 308 9 Cuts at: 2782 4867 136 Size: 2085 1525 Fragments arranged by size: 2085 1525 793 631 605 308 122 107 71 9 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 2843 3551 3673 2843 Size: 708 122 5426 Fragments arranged by size: 5426 708 122 Bme1580I G_kGCm'C Cuts at: 1790 2147 2235 2357 2893 3089 3099 3133 3153 Size: 357 88 122 536 196 10 34 20 Cuts at: 3153 3237 3537 4317 5473 1790 Size: 84 300 780 1156 2573 Fragments arranged by size: 2573 1156 780 536 357 300 196 122 88 84 34 20 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 3169 3203 Size: 416 53 83 399 24 646 1106 34 Cuts at: 3203 3987 4945 442 Size: 784 958 1753 Fragments arranged by size: 1753 1106 958 784 646 416 399 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2234 2330 2459 2738 2747 3284 3425 Size: 803 192 96 129 279 9 537 141 Cuts at: 3425 4916 1239 Size: 1491 2579 Fragments arranged by size: 2579 1491 803 537 279 192 141 129 96 9 Bpu10I CC'TnA_GC Cuts at: 2201 2201 Size: 6256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 4381 5246 5544 2393 Size: 1988 865 298 3105 Fragments arranged by size: 3105 1988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2548 4919 882 Size: 617 1049 2371 2219 Fragments arranged by size: 2371 2219 1049 617 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 3198 4063 4445 Size: 53 83 186 936 642 1023 865 382 Cuts at: 4445 5080 275 Size: 635 1451 Fragments arranged by size: 1451 1023 936 865 642 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2944 4689 5087 5361 5508 5919 2944 Size: 1745 398 274 147 411 3281 Fragments arranged by size: 3281 1745 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6031 Fragments arranged by size: 6031 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2617 2666 2750 2852 2986 3467 1186 Size: 853 578 49 84 102 134 481 3975 Fragments arranged by size: 3975 853 578 481 134 102 84 49 BseYI C'CCAG_C Cuts at: 2421 2448 2821 3229 3324 3417 5459 5955 2421 Size: 27 373 408 95 93 2042 496 2722 Fragments arranged by size: 2722 2042 496 408 373 95 93 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2365 3244 1163 Size: 573 629 879 4175 Fragments arranged by size: 4175 879 629 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2306 3706 4467 5495 1540 Size: 275 186 305 1400 761 1028 2301 Fragments arranged by size: 2301 1400 1028 761 305 275 186 BsiHKAI G_wGCw'C Cuts at: 2316 2357 2715 3114 3552 4317 4402 5473 2316 Size: 41 358 399 438 765 85 1071 3099 Fragments arranged by size: 3099 1071 765 438 399 358 85 41 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 BsmBI CGTCTCn'nnnn_ Cuts at: 2640 3205 2640 Size: 565 5691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2147 2163 2235 2316 2357 2715 Size: 399 268 89 16 72 81 41 358 Cuts at: 2715 2893 3089 3099 3114 3133 3153 3237 3279 Size: 178 196 10 15 19 20 84 42 Cuts at: 3279 3411 3537 3552 3575 4317 4402 5473 1391 Size: 132 126 15 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 268 196 178 132 126 89 85 84 81 72 42 41 23 20 19 16 15 15 10 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2045 2713 3049 3109 3611 844 Size: 1201 668 336 60 502 3489 Fragments arranged by size: 3489 1201 668 502 336 60 BsrBI CCG'CTC Cuts at: 1394 1823 2383 4143 1394 Size: 429 560 1760 3507 Fragments arranged by size: 3507 1760 560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3131 4751 4925 65 Size: 1183 1883 1620 174 1396 Fragments arranged by size: 1883 1620 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2847 2944 4900 5087 1700 Size: 110 1037 97 1956 187 2869 Fragments arranged by size: 2869 1956 1037 187 110 97 BsrGI T'GTAC_A Cuts at: 96 3426 96 Size: 3330 2926 BssHII G'CGCG_C Cuts at: 1925 2224 2282 3169 1925 Size: 299 58 887 5012 Fragments arranged by size: 5012 887 299 58 BssSI C'ACGA_G Cuts at: 2378 4316 5328 2378 Size: 1938 1012 3306 Fragments arranged by size: 3306 1938 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2352 2662 794 Size: 1558 310 4388 Fragments arranged by size: 4388 1558 310 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2443 2825 1184 Size: 1259 382 4615 Fragments arranged by size: 4615 1259 382 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2905 2050 Size: 855 5401 BstYI r'GATC_y Cuts at: 1972 2242 2508 2703 3586 3953 4338 4355 5807 Size: 270 266 195 883 367 385 17 1452 Cuts at: 5807 5893 5905 6251 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 883 385 367 346 270 266 195 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3379 3379 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2004 2296 2692 2919 3218 513 Size: 552 939 292 396 227 299 3551 Fragments arranged by size: 3551 939 552 396 299 292 227 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 2807 3772 4566 4586 2807 Size: 965 794 20 4477 Fragments arranged by size: 4477 965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DraIII CAC_nnn'GTG Cuts at: 3187 3187 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2086 2303 Size: 54 1425 50 275 18 177 79 217 Cuts at: 2303 2616 3253 3703 4592 6221 8 Size: 313 637 450 889 1629 43 Fragments arranged by size: 1629 1425 889 637 450 313 275 217 177 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2303 3703 1537 Size: 275 491 1400 4090 Fragments arranged by size: 4090 1400 491 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2865 3449 3537 4186 1353 Size: 968 544 584 88 649 3423 Fragments arranged by size: 3423 968 649 584 544 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2314 2713 3112 2314 Size: 399 399 5458 Fragments arranged by size: 5458 399 399 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2234 2330 2459 2738 2747 2852 Size: 393 410 192 96 129 279 9 105 Cuts at: 2852 3284 3425 4319 4916 5703 5971 1239 Size: 432 141 894 597 787 268 1524 Fragments arranged by size: 1524 894 787 597 432 410 393 279 268 192 141 129 105 96 9 EcoNI CCTnn'n_nnAGG Cuts at: 2567 2720 2567 Size: 153 6103 EcoO109I rG'GnC_Cy Cuts at: 2065 2103 2572 2774 2926 2933 3085 3233 3234 Size: 38 469 202 152 7 152 148 1 Cuts at: 3234 3260 3489 3533 3534 2065 Size: 26 229 44 1 4787 Fragments arranged by size: 4787 469 229 202 152 152 148 44 38 26 7 1 1 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 2492 4762 2492 Size: 2270 3986 HaeII r_GCGC'y Cuts at: 1678 2178 2390 2407 2633 3024 3331 5403 1678 Size: 500 212 17 226 391 307 2072 2531 Fragments arranged by size: 2531 2072 500 391 307 226 212 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2995 3027 3362 3394 4897 Size: 32 135 32 1423 32 335 32 1503 Cuts at: 4897 4929 4971 5003 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1503 1423 335 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2174 2174 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 10 Size: 54 1425 343 786 3648 Fragments arranged by size: 3648 1425 786 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 4214 4573 4732 518 Size: 648 1446 1602 359 159 2042 Fragments arranged by size: 2042 1602 1446 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2130 2205 2308 2337 2425 2659 2821 Size: 411 113 75 103 29 88 234 162 Cuts at: 2821 3672 4349 5497 5742 1606 Size: 851 677 1148 245 2120 Fragments arranged by size: 2120 1148 851 677 411 245 234 162 113 103 88 75 29 NaeI GCC'GGC Cuts at: 2849 2849 Size: 6256 NarI GG'CG_CC Cuts at: 2175 2175 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 2004 2692 513 Size: 552 939 688 4077 Fragments arranged by size: 4077 939 688 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2847 2847 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 2303 3703 2303 Size: 1400 4856 NspI r_CATG'y Cuts at: 1132 1229 3614 5159 1132 Size: 97 2385 1545 2229 Fragments arranged by size: 2385 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2771 2885 3335 1348 Size: 1423 114 450 4269 Fragments arranged by size: 4269 1423 450 114 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2821 1108 Size: 1713 4543 PfoI T'CCnGG_A Cuts at: 2112 2706 2715 3285 2112 Size: 594 9 570 5083 Fragments arranged by size: 5083 594 570 9 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2933 3260 3489 2065 Size: 868 327 229 4832 Fragments arranged by size: 4832 868 327 229 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 2231 3085 3095 3233 3533 2231 Size: 854 10 138 300 4954 Fragments arranged by size: 4954 854 300 138 10 PspXI vC'TCGA_Gb Cuts at: 3479 3479 Size: 6256 PstI C_TGCA'G Cuts at: 830 2702 2748 2802 3608 830 Size: 1872 46 54 806 3478 Fragments arranged by size: 3478 1872 806 54 46 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2017 2425 2821 2017 Size: 408 396 5452 Fragments arranged by size: 5452 408 396 RsrII CG'GwC_CG Cuts at: 2325 2325 Size: 6256 SacI G_AGCT'C Cuts at: 2316 2715 3114 2316 Size: 399 399 5458 Fragments arranged by size: 5458 399 399 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SanDI GG'GwC_CC Cuts at: 3260 3260 Size: 6256 SapI GCTCTTCn'nnn_ Cuts at: 2321 2321 Size: 6256 SbfI CC_TGCA'GG Cuts at: 2748 3608 2748 Size: 860 5396 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SfcI C'TryA_G Cuts at: 826 1045 2567 2698 2744 2798 3397 3604 4739 Size: 219 1522 131 46 54 599 207 1135 Cuts at: 4739 5420 5611 826 Size: 681 191 1471 Fragments arranged by size: 1522 1471 1135 681 599 219 207 191 131 54 46 SfiI GGCCn_nnn'nGGCC Cuts at: 2465 2782 2465 Size: 317 5939 SfoI GGC'GCC Cuts at: 2176 2176 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2240 3083 3583 1872 Size: 368 843 500 4545 Fragments arranged by size: 4545 843 500 368 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2643 3479 4360 5261 5523 Size: 197 926 465 235 836 881 901 262 Cuts at: 5523 820 Size: 1553 Fragments arranged by size: 1553 926 901 881 836 465 262 235 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 3305 1517 Size: 1788 4468 StyI C'CwwG_G Cuts at: 513 1065 2004 2692 3695 513 Size: 552 939 688 1003 3074 Fragments arranged by size: 3074 1003 939 688 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 2477 2813 4283 4300 4453 4638 Size: 1255 762 135 336 1470 17 153 185 Cuts at: 4638 5083 325 Size: 445 1498 Fragments arranged by size: 1498 1470 1255 762 445 336 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3426 4502 6214 Size: 274 80 33 51 1443 1449 1076 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 1712 1449 1443 1076 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2947 2979 6216 6248 2947 Size: 32 3237 32 2955 Fragments arranged by size: 3237 2955 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 2643 3479 1943 Size: 700 836 4720 Fragments arranged by size: 4720 836 700 XmaI C'CCGG_G Cuts at: 1870 2238 3081 3581 1870 Size: 368 843 500 4545 Fragments arranged by size: 4545 843 500 368 XmnI GAAnn'nnTTC Cuts at: 4385 4385 Size: 6256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI SacII SexAI SrfI SwaI XcmI