(Linear) (Six-base) MAPSORT of: KIEE4126.seq Check: 3250 from: 1 to: 1551 KIEE4126.orf With 182 enzymes: SgfI * March 22, 2012 17:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 708 1044 1104 1551 Size: 708 336 60 447 Fragments arranged by size: 708 447 336 60 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 847 1551 Size: 847 704 AgeI A'CCGG_T Cuts at: 0 939 1551 Size: 939 612 AlwNI CAG_nnn'CTG Cuts at: 0 15 570 627 657 1419 1513 1551 Size: 15 555 57 30 762 94 38 Fragments arranged by size: 762 555 94 57 38 30 15 ApaI G_GGCC'C Cuts at: 0 230 1084 1094 1232 1532 1551 Size: 230 854 10 138 300 19 Fragments arranged by size: 854 300 230 138 19 10 ApaLI G'TGCA_C Cuts at: 0 348 1551 Size: 348 1203 AvaI C'yCGr_G Cuts at: 0 142 233 638 1076 1474 1551 Size: 142 91 405 438 398 77 Fragments arranged by size: 438 405 398 142 91 77 BamHI G'GATC_C Cuts at: 0 237 698 1551 Size: 237 461 853 Fragments arranged by size: 853 461 237 BanI G'GyrC_C Cuts at: 0 169 885 936 1145 1551 Size: 169 716 51 209 406 Fragments arranged by size: 716 406 209 169 51 BanII G_rGCy'C Cuts at: 0 53 158 230 311 710 1084 1094 1109 Size: 53 105 72 81 399 374 10 15 Cuts at: 1109 1232 1274 1406 1532 1551 Size: 123 42 132 126 19 Fragments arranged by size: 399 374 132 126 123 105 81 72 53 42 19 15 10 BbeI G_GCGC'C Cuts at: 0 173 1551 Size: 173 1378 BbsI GAAGACnn'nnnn_ Cuts at: 0 812 1455 1551 Size: 812 643 96 Fragments arranged by size: 812 643 96 BbvCI CC'TCA_GC Cuts at: 0 196 1551 Size: 196 1355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 970 1004 1551 Size: 970 34 547 Fragments arranged by size: 970 547 34 BglI GCCn_nnn'nGGC Cuts at: 0 460 768 777 1551 Size: 460 308 9 774 Fragments arranged by size: 774 460 308 9 BlpI GC'TnA_GC Cuts at: 0 838 1546 1551 Size: 838 708 5 Fragments arranged by size: 838 708 5 Bme1580I G_kGCm'C Cuts at: 0 142 230 352 888 1084 1094 1128 1148 Size: 142 88 122 536 196 10 34 20 Cuts at: 1148 1232 1532 1551 Size: 84 300 19 Fragments arranged by size: 536 300 196 142 122 88 84 34 20 19 10 BmrI ACTGGGnnnn_n' Cuts at: 0 58 1164 1198 1551 Size: 58 1106 34 353 Fragments arranged by size: 1106 353 58 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 229 325 454 733 742 1279 1420 Size: 37 192 96 129 279 9 537 141 Cuts at: 1420 1551 Size: 131 Fragments arranged by size: 537 279 192 141 131 129 96 37 9 Bpu10I CC'TnA_GC Cuts at: 0 196 1551 Size: 196 1355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1551 Size: 388 1163 BsaI GGTCTCn'nnnn_ Cuts at: 0 543 1551 Size: 543 1008 BsaHI Gr'CG_yC Cuts at: 0 170 1193 1551 Size: 170 1023 358 Fragments arranged by size: 1023 358 170 BsaWI w'CCGG_w Cuts at: 0 939 1551 Size: 939 612 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 612 661 745 847 981 1462 1551 Size: 34 578 49 84 102 134 481 89 Fragments arranged by size: 578 481 134 102 89 84 49 34 BseYI C'CCAG_C Cuts at: 0 416 443 816 1224 1319 1412 1551 Size: 416 27 373 408 95 93 139 Fragments arranged by size: 416 408 373 139 95 93 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 1239 1551 Size: 360 879 312 Fragments arranged by size: 879 360 312 BsiEI CG_ry'CG Cuts at: 0 301 1551 Size: 301 1250 BsiHKAI G_wGCw'C Cuts at: 0 311 352 710 1109 1547 1551 Size: 311 41 358 399 438 4 Fragments arranged by size: 438 399 358 311 41 4 BsmBI CGTCTCn'nnnn_ Cuts at: 0 635 1200 1551 Size: 635 565 351 Fragments arranged by size: 635 565 351 Bsp1286I G_dGCh'C Cuts at: 0 53 142 158 230 311 352 710 888 Size: 53 89 16 72 81 41 358 178 Cuts at: 888 1084 1094 1109 1128 1148 1232 1274 1406 Size: 196 10 15 19 20 84 42 132 Cuts at: 1406 1532 1547 1551 Size: 126 15 4 Fragments arranged by size: 358 196 178 132 126 89 84 81 72 53 42 41 20 19 16 15 15 10 4 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 40 708 1044 1104 1551 Size: 40 668 336 60 447 Fragments arranged by size: 668 447 336 60 40 BsrBI CCG'CTC Cuts at: 0 378 1551 Size: 378 1173 BsrDI GCAATG_nn' Cuts at: 0 1126 1551 Size: 1126 425 BsrFI r'CCGG_y Cuts at: 0 842 939 1551 Size: 842 97 612 Fragments arranged by size: 842 612 97 BsrGI T'GTAC_A Cuts at: 0 1421 1551 Size: 1421 130 BssHII G'CGCG_C Cuts at: 0 219 277 1164 1551 Size: 219 58 887 387 Fragments arranged by size: 887 387 219 58 BssSI C'ACGA_G Cuts at: 0 373 1551 Size: 373 1178 BstAPI GCAn_nnn'nTGC Cuts at: 0 347 657 1551 Size: 347 310 894 Fragments arranged by size: 894 347 310 BstEII G'GTnAC_C Cuts at: 0 438 820 1551 Size: 438 382 731 Fragments arranged by size: 731 438 382 BstXI CCAn_nnnn'nTGG Cuts at: 0 45 900 1551 Size: 45 855 651 Fragments arranged by size: 855 651 45 BstYI r'GATC_y Cuts at: 0 237 503 698 1551 Size: 237 266 195 853 Fragments arranged by size: 853 266 237 195 Bsu36I CC'TnA_GG Cuts at: 0 1374 1551 Size: 1374 177 BtgI C'CryG_G Cuts at: 0 291 687 914 1213 1551 Size: 291 396 227 299 338 Fragments arranged by size: 396 338 299 291 227 BtsI GCAGTG_nn' Cuts at: 0 802 1551 Size: 802 749 DraIII CAC_nnn'GTG Cuts at: 0 1182 1551 Size: 1182 369 EaeI y'GGCC_r Cuts at: 0 2 81 298 611 1248 1551 Size: 2 79 217 313 637 303 Fragments arranged by size: 637 313 303 217 79 2 EagI C'GGCC_G Cuts at: 0 298 1551 Size: 298 1253 EarI CTCTTCn'nnn_ Cuts at: 0 316 860 1444 1532 1551 Size: 316 544 584 88 19 Fragments arranged by size: 584 544 316 88 19 EcoICRI GAG'CTC Cuts at: 0 309 708 1107 1551 Size: 309 399 399 444 Fragments arranged by size: 444 399 399 309 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 229 325 454 733 742 847 1279 Size: 37 192 96 129 279 9 105 432 Cuts at: 1279 1420 1551 Size: 141 131 Fragments arranged by size: 432 279 192 141 131 129 105 96 37 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 562 715 1551 Size: 562 153 836 Fragments arranged by size: 836 562 153 EcoO109I rG'GnC_Cy Cuts at: 0 60 98 567 769 921 928 1080 1228 Size: 60 38 469 202 152 7 152 148 Cuts at: 1228 1229 1255 1484 1528 1529 1551 Size: 1 26 229 44 1 22 Fragments arranged by size: 469 229 202 152 152 148 60 44 38 26 22 7 1 1 FspI TGC'GCA Cuts at: 0 487 1551 Size: 487 1064 HaeII r_GCGC'y Cuts at: 0 173 385 402 628 1019 1326 1551 Size: 173 212 17 226 391 307 225 Fragments arranged by size: 391 307 226 225 212 173 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 990 1022 1357 1389 1551 Size: 990 32 335 32 162 Fragments arranged by size: 990 335 162 32 32 KasI G'GCGC_C Cuts at: 0 169 1551 Size: 169 1382 MscI TGG'CCA Cuts at: 0 613 1551 Size: 613 938 MslI CAynn'nnrTG Cuts at: 0 607 1551 Size: 607 944 MspA1I CmG'CkG Cuts at: 0 12 125 200 303 332 420 654 816 Size: 12 113 75 103 29 88 234 162 Cuts at: 816 1551 Size: 735 Fragments arranged by size: 735 234 162 113 103 88 75 29 12 NaeI GCC'GGC Cuts at: 0 844 1551 Size: 844 707 NarI GG'CG_CC Cuts at: 0 170 1551 Size: 170 1381 NcoI C'CATG_G Cuts at: 0 687 1551 Size: 687 864 NgoMIV G'CCGG_C Cuts at: 0 842 1551 Size: 842 709 NotI GC'GGCC_GC Cuts at: 0 298 1551 Size: 298 1253 PasI CC'CwG_GG Cuts at: 0 766 880 1330 1551 Size: 766 114 450 221 Fragments arranged by size: 766 450 221 114 PflMI CCAn_nnn'nTGG Cuts at: 0 816 1551 Size: 816 735 PfoI T'CCnGG_A Cuts at: 0 107 701 710 1280 1551 Size: 107 594 9 570 271 Fragments arranged by size: 594 570 271 107 9 PpuMI rG'GwC_Cy Cuts at: 0 60 928 1255 1484 1551 Size: 60 868 327 229 67 Fragments arranged by size: 868 327 229 67 60 PspOMI G'GGCC_C Cuts at: 0 226 1080 1090 1228 1528 1551 Size: 226 854 10 138 300 23 Fragments arranged by size: 854 300 226 138 23 10 PspXI vC'TCGA_Gb Cuts at: 0 1474 1551 Size: 1474 77 PstI C_TGCA'G Cuts at: 0 697 743 797 1551 Size: 697 46 54 754 Fragments arranged by size: 754 697 54 46 PvuII CAG'CTG Cuts at: 0 12 420 816 1551 Size: 12 408 396 735 Fragments arranged by size: 735 408 396 12 RsrII CG'GwC_CG Cuts at: 0 320 1551 Size: 320 1231 SacI G_AGCT'C Cuts at: 0 311 710 1109 1551 Size: 311 399 399 442 Fragments arranged by size: 442 399 399 311 SanDI GG'GwC_CC Cuts at: 0 1255 1551 Size: 1255 296 SapI GCTCTTCn'nnn_ Cuts at: 0 316 1551 Size: 316 1235 SbfI CC_TGCA'GG Cuts at: 0 743 1551 Size: 743 808 SfcI C'TryA_G Cuts at: 0 562 693 739 793 1392 1551 Size: 562 131 46 54 599 159 Fragments arranged by size: 599 562 159 131 54 46 SfiI GGCCn_nnn'nGGCC Cuts at: 0 460 777 1551 Size: 460 317 774 Fragments arranged by size: 774 460 317 SfoI GGC'GCC Cuts at: 0 171 1551 Size: 171 1380 SmaI CCC'GGG Cuts at: 0 235 1078 1551 Size: 235 843 473 Fragments arranged by size: 843 473 235 SmlI C'TyrA_G Cuts at: 0 403 638 1474 1551 Size: 403 235 836 77 Fragments arranged by size: 836 403 235 77 StuI AGG'CCT Cuts at: 0 1300 1551 Size: 1300 251 StyI C'CwwG_G Cuts at: 0 687 1551 Size: 687 864 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 337 472 808 1551 Size: 337 135 336 743 Fragments arranged by size: 743 337 336 135 TatI w'GTAC_w Cuts at: 0 1421 1551 Size: 1421 130 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 942 974 1551 Size: 942 32 577 Fragments arranged by size: 942 577 32 XhoI C'TCGA_G Cuts at: 0 638 1474 1551 Size: 638 836 77 Fragments arranged by size: 836 638 77 XmaI C'CCGG_G Cuts at: 0 233 1076 1551 Size: 233 843 475 Fragments arranged by size: 843 475 233 Enzymes that do cut: AarI AcuI AgeI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII RsrII SacI SanDI SapI SbfI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BsaXI BsiWI BsmI BspEI BspHI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI SacII SalI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XmnI ZraI